| Project/Area Number |
22791485
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Nagoya City University |
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Principal Investigator |
NAKANE Akihiro 名古屋市立大学, 大学院・医学研究科, 研究員 (70464568)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 再生医療 / 胚性幹細胞 / 遺伝子導入 / 腎再生 / Pax2遺伝子 / 再生医学 / 分化実験 / RT-PCR |
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| Research Abstract |
We have generated ES cell lines that repress Pax2 expression and examined their differentiation potential by embryoid body(EB) formation. EBs were cultured with retinoic acid and activin A. EBs were analyzed by reverse transcription(RT)-PCR and immunocytochemical analysis. EBs expressed Pax2 and subsequently integrin α8 and AQP1.With retinoic acid and activin A, EBs overexpressed Pax2-induced BMP7, Pax8 and Podocin, ES cell lines with inducible Pax2 expression may be useful for dissecting genetic cascades functioning in the development of various organs, including the kidney, as well as for screening candidate genes that direct ES cells toward the desired lineages, which may be applicable to future cell therapies.
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