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The basic research about the investigation of the mechanism about the kidney generation and the application to kidney regeneration medicine using Pax2-recombinant embryonic stem cells.

Research Project

Project/Area Number 22791485
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionNagoya City University

Principal Investigator

NAKANE Akihiro  名古屋市立大学, 大学院・医学研究科, 研究員 (70464568)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords再生医療 / 胚性幹細胞 / 遺伝子導入 / 腎再生 / Pax2遺伝子 / 再生医学 / 分化実験 / RT-PCR
Research Abstract

We have generated ES cell lines that repress Pax2 expression and examined their differentiation potential by embryoid body(EB) formation. EBs were cultured with retinoic acid and activin A. EBs were analyzed by reverse transcription(RT)-PCR and immunocytochemical analysis. EBs expressed Pax2 and subsequently integrin α8 and AQP1.With retinoic acid and activin A, EBs overexpressed Pax2-induced BMP7, Pax8 and Podocin, ES cell lines with inducible Pax2 expression may be useful for dissecting genetic cascades functioning in the development of various organs, including the kidney, as well as for screening candidate genes that direct ES cells toward the desired lineages, which may be applicable to future cell therapies.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (8 results)

All 2011 2010

All Presentation (7 results) Book (1 results)

  • [Presentation] Pax2 overexpression in embryoid bodies induces upregulation of genes with retinoic acid and activin a involved in kidney development2011

    • Author(s)
      Akihiro Nakane
    • Organizer
      AUA annual meting 2011
    • Place of Presentation
      Washington Convention Center (U.S.A)
    • Year and Date
      2011-05-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新たなる腎再生への挑戦2011

    • Author(s)
      中根明宏
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県)(招待講演)
    • Year and Date
      2011-04-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 腎再生医療に向けたPax2遺伝子導入による腎細胞への分化誘導2011

    • Author(s)
      中根明宏
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-04-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Pax2 overexpression in embryoid bodies induces upregulation of genes with retinoic acid and activin a involved in kidney development2011

    • Author(s)
      Nakane Akihiro, Nishinakamura Ryoichi, Mizuno Kentaro, Kojima Yoshiyuki, Maruyama Tetsuji, Hayashi Yutaro, Kohri Kenjiro
    • Organizer
      AUA 2011
    • Place of Presentation
      Washington, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] 腎再生医療に向けたPax遺伝子導入による腎細胞への分化誘導2011

    • Author(s)
      中根明宏、神沢英幸、黒川覚史、佐々木昌一、林祐太郎、西中村隆一、郡健二郎
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋市
    • Related Report
      2011 Final Research Report
  • [Presentation] PAX2-overexpression in embryoid bodies induces upregulation of Integrin a8 and aquaporin-1 involved in kidney development2010

    • Author(s)
      中根明宏
    • Organizer
      AUA 2010 Annual Meeting
    • Place of Presentation
      MOSCONE CENTER, San Francisco, USA
    • Year and Date
      2010-05-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] PAX2 overexpression in embryoid bodies induces upregulation of Integrin a8 and aquaporin-1 involved in kidney development2010

    • Author(s)
      Nakane Akihiro, Nishinakamura Ryuichi, Mizuno Kentaro, Kojima Yoshiyuki, Maruyama Tetsuji, Hayashi Yutaro, Konri Kenjiro
    • Organizer
      AUA 2010 Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2011 Final Research Report
  • [Book] 泌尿器科レジデントマニュアル(監修郡健二郎、編集佐々木昌一、戸澤啓一、丸山哲史)2011

    • Author(s)
      中根明宏
    • Publisher
      医学書院
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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