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Establishment of new prostate cancer metastatic model with androgen independent growth and identification of new mechanism of androgen-independency

Research Project

Project/Area Number 22791487
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionNagoya City University

Principal Investigator

NAIKI Taku  名古屋市立大学, 大学院・医学研究科, 臨床研究医 (50551272)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords去勢抵抗性前立腺癌 / 転移動物モデル / 分子標的治療 / 前立腺癌 / 動物モデル
Research Abstract

We established androgen-independent prostate cancer cell line from TRAP tumor, and named PCai1, and previously reported the orthotopic metastatic models, and tail vein injection model using PCai1 cells. We investigate the GST-P expression of PCai1 by RT-PCR and Western blot. In addition, using GST-P-siRNA, we investigated the relationship between the cell growth and Reactive Oxygen Species(ROS) in PCai1.PCai1 cells had higher GST-P expression in mRNA and protein levels in Charcoal Stripped-FBS medium than in normal medium. GST-P knocked down by siRNA resulted in significant decrease of the proliferation rate in vitro. On the contrary, DCFH assay revealed that ROS was induced by GST-P-siRNA treat. Tumors metastasized in the bone marrow after intravenous injection expressed high levels of GST-P, while metastatic lesions in the lung and lymph nodes failed to express GST-P as seen in the case of the orthotopic implantation. GST-P might have the important roles in the prostate cancer growth and metastasis by the restriction of ROS.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (13 results)

All 2012 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (8 results)

  • [Journal Article] Organ specific Gst-pi expression of the metastatic androgen inde pendent prostate cancer cells in nude mice2012

    • Author(s)
      Naiki T
    • Journal Title

      The Prostate

      Volume: 72 Pages: 533-541

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organ specific Gst-pi expression of the metastatic androgen independent prostate cancer cells in nude mice2011

    • Author(s)
      Naiki T, Asamoto M, Toyoda-Hokaiwado N, Naiki-Ito A, Tozawa K, Kohri K, Takahashi S, Shirai T
    • Journal Title

      The Prostate

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preoperative Prediction of Neurovascular Bundle Involvement of Localized Prostate Cancer by Combined T2 and Diffusion-weighted Imaging of Magnetic Resonance Imaging, Number of Positive Biopsy Cores, and Gleason Score2011

    • Author(s)
      Naiki T, Okamura T, Nagata D, Mori Y, Kawai N, Ogawa K, Akita H, Hashimoro Y, Tozawa K, Kohri K
    • Journal Title

      Asian Pac J Cancer Prev

      Volume: 12 Pages: 909-13

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preoperative Prediction of Neurovascular Bundle Involvement of Localized Prostate Cancer by Combined T2 and Diffusion-weighted Imaging of Magnetic Resonance Imaging, Number of Positive Biopsy Cores, and Gleason Score2011

    • Author(s)
      Naiki T
    • Journal Title

      Asian Pac J Cancer Prev

      Volume: 12 Pages: 909-913

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tranilast suppresses prostate cancer growth and differentiation in vivo and in vitro2010

    • Author(s)
      Sato S, Takahashi S, Asamoto M, Naiki T, Naiki-Ito A, Asai K, Shirai T
    • Journal Title

      The Prostate

      Volume: 70 Pages: 229-38

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] Glutathione oxidation-reduction system facilitates androgen independent prostate cancer growth2011

    • Author(s)
      Naiki T, Asamoto M, Naiki A, Kawai N, Tozawa K, Kohri K
    • Organizer
      第70回日本癌学会総会
    • Place of Presentation
      名古屋市
    • Related Report
      2011 Final Research Report
  • [Presentation] 再燃前立腺癌における酸化ストレス応答因子の役割2011

    • Author(s)
      内木拓, 朝元誠人, 内木綾, 小島祥敬, 河合憲康, 戸澤啓一, 白井智之, 郡健二郎
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋市
    • Related Report
      2011 Final Research Report
  • [Presentation] Glutathione oxidation-reduction system facilitates androgen independent prostate cancer growth2011

    • Author(s)
      Naiki T
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 再燃前立腺癌における酸化ストレス応答因子の役割2011

    • Author(s)
      内木拓
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Metastatic site specific different expression of GST-P in new androgen independent prostate cancer cells from TRAP model2010

    • Author(s)
      Naiki T, Asamoto M, Naiki-Ito A, Tozawa K, Kohri K, Shirai T
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪市・リーガロイヤルホテル大阪
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] ラット前立腺癌モデル由来細胞株によるアンドロゲン非依存性前立腺癌転移モデルの確立2010

    • Author(s)
      内木拓、朝元誠人、内木綾、戸澤啓一、白井智之、郡健二郎
    • Organizer
      第98回日本泌尿器科学会総会
    • Place of Presentation
      岩手県盛岡市・いわて県民情報交流センター「アイーナ」
    • Year and Date
      2010-04-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Metastatic site specific different expression of GST-P in new androgen independent prostate cancer cells from TRAP model2010

    • Author(s)
      Naiki T, Asamoto M, Naiki-Ito A, Tozawa K, Kohri K, Shirai T
    • Organizer
      第69回日本癌学会学術総会シンポジウム
    • Place of Presentation
      大阪市
    • Related Report
      2011 Final Research Report
  • [Presentation] ラット前立腺癌モデル由来細胞株によるアンドロゲン非依存性前立腺癌転移モデルの確立2010

    • Author(s)
      内木拓, 朝元誠人, 内木綾, 戸澤啓一, 白井智之, 郡健二郎
    • Organizer
      第98回日本泌尿器科学会総会
    • Place of Presentation
      盛岡市
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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