Establishing orthotopic model of prostate cancer metastasis to bone in mice

• Project/Area Number: 22791497
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Keio University
• Principal Investigator: KANAI Kunimitsu 慶應義塾大学, 医学部, 共同研究員 (80365361)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 前立腺癌 / 骨転移モデル

Research Abstract

TRAMP-C1 is derived from a prostate adenocarcinoma in transgenic C57BL/6 mice. TRAMP-C1/CBR cells were genetically modified to express the luciferase gene. Nude mice(BALB/c, nu/nu) were anesthetized and received injection of TRAMP-C1/CBR cells with/without mesenchymal stem cells(MSC) in the prostatic lobe through a midline lower abdominal incision. To detect cancer development in mice, we used bioluminescence imaging. In orthotopic model, we generally detected a hot spot of bioluminescence in lower abdomen of mice. By the combination injection of TRAMP-C1 and MSC, the local hot spot was remarkably enlarged, however, we could not confirm the hot spot to prove the bone metastasis.