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Establishing orthotopic model of prostate cancer metastasis to bone in mice

Research Project

Project/Area Number 22791497
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionKeio University

Principal Investigator

KANAI Kunimitsu  慶應義塾大学, 医学部, 共同研究員 (80365361)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords前立腺癌 / 骨転移モデル
Research Abstract

TRAMP-C1 is derived from a prostate adenocarcinoma in transgenic C57BL/6 mice. TRAMP-C1/CBR cells were genetically modified to express the luciferase gene. Nude mice(BALB/c, nu/nu) were anesthetized and received injection of TRAMP-C1/CBR cells with/without mesenchymal stem cells(MSC) in the prostatic lobe through a midline lower abdominal incision. To detect cancer development in mice, we used bioluminescence imaging. In orthotopic model, we generally detected a hot spot of bioluminescence in lower abdomen of mice. By the combination injection of TRAMP-C1 and MSC, the local hot spot was remarkably enlarged, however, we could not confirm the hot spot to prove the bone metastasis.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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