Study of the mechanism for controlling of sperm function via membrane structure with proteins secreted by seminal vesicle

• Project/Area Number: 22791502
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Toin University of Yokohama
• Principal Investigator: YOSHIDA Kaoru 桐蔭横浜大学, 先端医用工学センター, 講師 (70398973)
• Co-Investigator(Renkei-kenkyūsha): IWAMOTO Teruaki 国際医療福祉大学, 大学病院, 教授 (60046117) ; YOSHIDA Manabu 東京大学, 大学院・理学系研究科, 准教授 (60301785)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 精子 / 受精能 / 膜ラフト / 糖脂質 / 膜タンパク質 / コレステロール / アンドロロジー / ミトコンドリア

Research Abstract

Seminal vesicle secreted protein, semenogelins(SEMGs) are one of inhibitory factor for hyperactivation of sperm motility. Our previous study has revealed that SEMGs were also inhibitory factors for acrosome reaction and protein tyrosin phosphorylation. Collectively, SEMGs may be inhibitory factors for sperm capacitation. However, it is still unclear that the direct interaction between lipid lafts and SEMGs. Our study suggests that SEMGs can bind to cholesterol and effect on sperm membrane fluidity and on function for capacitaion.

Research Products

• [Journal Article] Seminal vesicle protein SVS2 is required for sperm survival in the uterus (2014)
  • Author(s): N Kawano, N Araki, K Ybshida, THibino, N Ohnami, M Makino, S Kanai, H Hasuwa, M Yoshida, K Miyado, A Umezawa
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 111(11) Issue: 11 Pages: 4145-4150
  • DOI: 10.1073/pnas.1320715111
  • Peer Reviewed / Open Access
• [Journal Article] Keys to Sperm Maturation (2011)
  • Author(s): Kawano N, Yoshida K, Miyado K, and Yoshida M. Lipid Rafts
  • Journal Title: Fertilization, and Early Embryogenesis Journal of Lipids Volume: (Article ID 264706)
  • Peer Reviewed
• [Journal Article] Lipid Rafts : Keys to Sperm Maturation, Fertilization, and Early Embry ogenesis (2011)
  • Author(s): Natsuko Kawano, Kaoru Yoshida, Kenji Miyado, Manabu Yoshida
  • Journal Title: Journal of Lipids Volume: 2011
  • Peer Reviewed
• [Journal Article] Association of seminal plasma motility inhibitors/semenogelins with sperm in asthenozoospermia-infertile men (2010)
  • Author(s): Terai K, Yoshida K, Yoshiike M, Fujime M, Iwamoto T.
  • Journal Title: Urologia Internationalis Volume: 85 Pages: 209-215
  • Peer Reviewed
• [Presentation] 精漿タンパク質SEMGの精子の受精能評価マーカーとしての検討 (2013)
  • Author(s): 吉田薫, 山崎一恭, 吉池美紀, 岩本晃明
  • Organizer: 第58回日本生殖医学会学術総会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 精漿タンパク質ＳＥＭＧの精子の受精能評価マーカーとしての検討 (2013)
  • Author(s): 吉田薫 、山崎一恭、吉池美紀、岩本晃明。精漿タンパク質ＳＥＭＧの精子の受精能評価マーカーとしての検討 第58回 日本生殖医学会学術総会 2013年
  • Organizer: 第58回 日本生殖医学会学術総会
  • Place of Presentation: 神戸国際会議場
• [Presentation] マウス受精能獲得抑制因子SVS2の精子膜コレステロール維持作用 (2012)
  • Author(s): 荒木直也, 河野菜摘子,吉田薫,宮戸健二,吉田学
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学
• [Presentation] SVS2 inhibits sperm capacitation by regulating the efflux of cholesterol from sperm plasma membrane (2012)
  • Author(s): Araki N, Yoshida K, Kawano N, Miyado K, and Yoshida M
  • Organizer: The International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: Nagoya, Japan
• [Presentation] SVS2 inhibits sperm capacitation by regulating the efflux of cholesterol from sperm plasma membrane. (2012)
  • Author(s): Araki, N., Yoshida, K., Kawano, N., Miyado, K., and Yoshida, M.
  • Organizer: The International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: Nagoya , Japan
• [Presentation] 精嚢分泌タンパク SVS2 のマウス体内受精における役割について (2011)
  • Author(s): 河野菜摘子, 荒木直也, 吉田薫, 吉田学, 宮戸健二
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] マウス精子受精能獲得における精嚢分泌タンパク質SVSsの機能解析 (2011)
  • Author(s): 荒木直也, 吉田薫, 河野菜摘子, 宮戸健二, 吉田学
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 精嚢分泌タンパクSVS2のマウス体内受精における役割について (2011)
  • Author(s): 河野菜摘子、荒木直也、吉田薫、吉田学、宮戸健二
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] Evaluation of sperm quality based on SPMIs binding on ICSI outcome (2010)
  • Author(s): Terai K, Yoshida K, Yoshiike M, Iwamoto T, Matsuura R, Yoshida A
  • Organizer: 11th International Symposium on SPERMATOLOGY Okinawa 2010
  • Place of Presentation: Okinawa Convention Center, Japan
  • Year and Date: 2010-06-02
• [Presentation] Evaluation of sperm quality based on SPMIs binding on ICSI outcome (2010)
  • Author(s): Terai K, Yoshida K, Yoshiike M, Iwamoto T, Matsuura R, Yoshida A
  • Organizer: 11th International Symposium on SPERMATOLOGY Okinawa 2010
  • Place of Presentation: Okinawa Convention Center
  • Year and Date: 2010-06-02
• [Book] Effects of the Seminal Plasma Proteins Semenogelin (SEMG)/Seminal Vesicle Secretion 2 (SVS2) on Sperm Fertility."Human Spermatozoa : Maturation, Capacitation and Abnormalities" Ed : Lejeune T and Delvaux Pascal pp205-220. (2010)
  • Author(s): Yoshida K, Iwamoto T, Yoshida M
  • Total Pages: 545
  • Publisher: Nova Science Publishers
• [Remarks]
  • URL: http://www.cc.toin.ac.jp/univ/japanese/professor/bme/labot-kaoruy.html
• [Remarks]
  • URL: http://www.cc.toin.ac.jp/univ/japanese/professor/bme/labot-kaoruy.html