Regeneration of oocytes from frozen ovaries : establishment of novel method for efficient pregnancy

• Project/Area Number: 22791512
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Akita University
• Principal Investigator: KUMAZAWA Yukiyo 秋田大学, 大学院・医学系研究科, 助教 (70400504)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 原始卵胞 / 卵胞発育 / PTEN / PI3K / ゲノムインプリンティング / 卵巣凍結 / 卵子再生

Research Abstract

In this study, we could activate dormant primordial follicles following the in vitro treatment of ovarian fragments to stimulate PI3K/PKB signaling system. The transplantation of these cultured ovarian fragments allowed to grow the activated primordial follicles to preovulatory stage. We could retrieve mature oocytes from the preovulatory follicles and normal pups were delivered after in vitro fertilization and embryo transfer. This method is expected to develop a novel method for pregnancy by using autologous ooytes in patients who lack follicle development but have residual primordial follicles.

Research Products

• [Journal Article] T. Brain-derived neurotrophic factor/tyrosine kinase B signaling regulates human trophoblast growth in an in vivo animal model of ectopic pregnancy (2011)
  • Author(s): Kawamura K, Kawamura N, Kumazawa Y, Kumagai J, Fujimoto T, Tanaka T
  • Journal Title: Endocrinology Volume: 152 Issue: 3 Pages: 1090-1100
  • DOI: 10.1210/en.2010-1124
  • Peer Reviewed
• [Journal Article] Brain-derived neurotrophic factor/tyrosine kinase B signaling regulateshuman trophoblast growth in an in vivo animal model of ectopic pregnancy. (2011)
  • Author(s): Kawamura K, Kawamura N, Kumazawa Y, et al.
  • Journal Title: Endocrinology Volume: 152 Pages: 1090-1100
  • Peer Reviewed
• [Presentation] (2011)子宮腺筋症症例におけるIVFによる妊娠の予後 (2011)
  • Author(s): 熊澤由紀代, 白澤弘光, 熊谷仁, 兒玉英也, 寺田幸弘
  • Organizer: 第49回日本生殖医学会
  • Place of Presentation: 福島
  • Year and Date: 2011-10-15
• [Presentation] 子宮腺筋症症例におけるIVFによる妊娠の予後 (2011)
  • Author(s): 熊澤由紀代
  • Organizer: 第49回日本生殖医学会東北ブロック
  • Place of Presentation: 福島
  • Year and Date: 2011-10-15
• [Presentation] (2011)羊水過多症における羊水糖濃度測定の有用性 (2011)
  • Author(s): 熊澤由紀代, 小川正樹, 佐藤朗, 長尾大輔, 森耕太郎, 鎌田久美子, 寺田幸弘
  • Organizer: 第47回日本周産期・新生児医学会
  • Place of Presentation: 札幌
• [Presentation] (2011)腹腔鏡下手術後の痛みについての検討 (2011)
  • Author(s): 熊澤由紀代, 熊谷仁, 河村和弘, 白澤弘光, 児玉英也, 寺田幸弘
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪
• [Presentation] 羊水過多症における羊水糖濃度測定の有用性 (2011)
  • Author(s): 熊澤由紀代
  • Organizer: 第47回日本周産期・新生児医学会
  • Place of Presentation: 札幌
• [Presentation] 腹腔鏡下手術後の痛みについての検討 (2011)
  • Author(s): 熊澤由紀代
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪