Analysis of protein expression of uterine sarcoma by iTRAQ method

• Project/Area Number: 22791530
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: UEDA Yutaka 大阪大学, 医学系・研究科, 助教 (10346215)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 子宮平滑筋肉腫 / 蛋白質 / iTRAQ法 / 網羅的解析 / 子官平滑筋肉腫

Research Abstract

We identified Protein C whose expression was specifically increased in leiomyosarcoma compared to normal myometrium and Protein L whose expression was specifically reduced in leiomyosarcoma compared to normal myometrium, by iTRAQ method and immunohistochemistry using clinical samples of uterine leiomyosarcoma and normal myometrium. Currently we are analyzing functions of these proteins.