Expression and functional analysis of miRNAs in human placenta:molecular and cellar research of IUGR

• Project/Area Number: 22791553
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Nippon Medical School
• Principal Investigator: KIKUCHI Kunio 日本医科大学, 医学部, 講師 (70374676)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 産科学 / 胎盤 / microRNA / マイクロRNA

Research Abstract

The microRNAs (miRNAs) derived from the chromosome 19 miRNA cluster (C19MC) are exclusively expressed in the human placenta. The C19MC miRNAs examined in this study were predominantly expressed in villous trophoblast. Furthermore, we revealed that protein phosphatase 3, regulatory subunit B, alpha (PPP3R1), was a one of C19MC target using an in vitro experimental validation system. These data suggest that C19MC miRNAs is involved in the fine tuning of the Ca2+/calcineurin /NFAT (nuclear factor of activated T-cells) pathway in the villous trophoblast of the human placenta.

Research Products

• [Journal Article] SnoN/SKIL modulates proliferationthrough control of hsa-miR-720 transcription in esophageal cancer cells. (2011)
  • Author(s): Eriko Shinozuka, Masao Miyashita, Yoshiaki Mizuguchi, Ichiro Akagi, Kunio Kikuchi, Hiroshi Makino, Takeshi Matsutani, Nobutoshi Hagiwara, Tsutomu Nomura, Eiji Uchida, Toshihiro Takizawa.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 430(1) Issue: 1 Pages: 101-106
  • DOI: 10.1016/j.bbrc.2012.11.005
  • Peer Reviewed
• [Journal Article] Genes that Integrate multiple adipogenic signaling pathways in human mesenchymal stem cells (2011)
  • Author(s): Ito T, Tsuruta S, Tomita K, Kikuchi K, Yokoi T, Aizawa Y
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 409(4) Issue: 4 Pages: 786-791
  • DOI: 10.1016/j.bbrc.2011.05.089
  • Peer Reviewed
• [Journal Article] Relationship between altered expression levels of MIR21, MIR143, and MIR205 and clinicopathologic features of esophageal squamous cell carcinoma (2011)
  • Author(s): Akagi I, Miyashita M, Ishibashi O, Mishima T, Makino H, Nomu Uchida E, Takizawa T.
  • Journal Title: Dis Esophagus Volume: 24(7) Issue: 7 Pages: 523-530
  • DOI: 10.1111/j.1442-2050.2011.01177.x
  • Peer Reviewed
• [Journal Article] 絨毛細胞におけるマイクロRNA (2011)
  • Author(s): 菊池邦生
  • Journal Title: 臨床婦人科産科 Volume: 65 Pages: 254-259
• [Presentation] 瀧澤俊広microRNAの網羅的解析手法と生殖免疫学分野での応用 (2012)
  • Author(s): 菊池邦生,アリモハメド,岩城隼,吉武洋,瀧澤敬美,石川源,松原茂樹,竹下俊行
  • Organizer: 第27回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2012-12-09
• [Presentation] 絨毛外栄養膜における接着分子CD44 (2012)
  • Author(s): 高橋宏典,菊池邦生,大口昭英,薄井里英,桑田知之,松本久宣,佐藤幸保,藤原浩,松原茂樹,鈴木光明,瀧澤俊広
  • Organizer: 浸潤能の検討第27回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2012-12-09
• [Presentation] Methylation profile of C19MC-miRNA CpG island in trophoblast cell lines (2012)
  • Author(s): Kunio Kikuchi, Guangjin Qu, Hironori Takahashi, Tomoyuki Kuwata, Rie Usui, Akihide Ohkuchi, Shan-shun Luo, Shigeki Matsubara, Mitsuaki Suzuki, Toshihiro Takizawa
  • Organizer: IFPA 2012 Hiroshima Meeting
  • Place of Presentation: 広島県広島市
  • Year and Date: 2012-09-20
• [Presentation] CD44 accelerates trophobalstic invasion: in vitro invasion analysis using HTR8/SVneo cells (2012)
  • Author(s): Hironori Takahashi, Kunio Kikuchi, Tomoyuki Kuwata, Rie Usui, Akihide Ohkuchi, Hisanori Matsumoto, Yukiyasu Sato, Hiroshi Fujiwara, Kumiko Ui-Tei, Shigeki Matsubara, Mitsuaki Suzuki1, Toshihiro Takizawa
  • Organizer: IFPA 2012 Hiroshima Meeting
  • Place of Presentation: 広島県広島市
  • Year and Date: 2012-09-20
• [Presentation] BeWo-cell-derived miR-517a modulates the expression of PRKG1 in Jurkat cells via Exosomes. (2012)
  • Author(s): Md. Moksed Ali, Takami Takizawa, Kunio Kikuchi, Shigeru Saito, Toshihiro Takizawa
  • Organizer: IFPA 2012 Hiroshima Meeting
  • Place of Presentation: 広島県広島市
  • Year and Date: 2012-09-18
• [Presentation] Circulating placenta-specific miRNAs are delivered to maternal immune cells during pregnancy. (2012)
  • Author(s): Saori Kambe, Yoichi Ishida, Kunio Kikuchi, Hiroshi Yoshitake, Akihide Okuchi, Shigeki Matsubara, MitsuakiSuzuki, Shigeru Saito, Toshiyuki Takeshita, Toshihiro Takizawa
  • Organizer: IFPA 2012 Hiroshima Meeting
  • Place of Presentation: 広島県広島市
  • Year and Date: 2012-09-18
• [Presentation] 甲状腺微少浸潤型濾胞癌の術後遠隔転移を予測するためのバイオマーカーの開発:miR-221/222は有用な予測因子である (2012)
  • Author(s): 軸薗智雄,川本雅司,赤須東樹,廣川満良,菊池邦生,宮内昭,土屋眞一,瀧澤俊広,清水一雄
  • Organizer: 第24回日本内分泌外科学会総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2012-06-09
• [Presentation] 胎盤栄養膜における接着因子CD44の浸潤能と発現制御機構の解析 (2012)
  • Author(s): 高橋宏典,菊池邦生,竹下俊行,松原茂樹,鈴木光明,瀧澤俊広
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2012-04-15
• [Presentation] 食道扁平上皮癌におけるmiRNA発現と臨床病理学的意義の検討 (2012)
  • Author(s): 篠塚恵理子,宮下正夫,水口義昭,赤城一郎,菊池邦生,萩原信敏,松谷毅,牧野浩司,野村務,瀧澤俊広,内田英二
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉県幕張市
  • Year and Date: 2012-04-13
• [Presentation] 瀧澤俊広胎盤由来のマイクロRNAは妊娠期間中エクソソームを介して母体血球細胞に取り込まれている (2012)
  • Author(s): 神戸沙織,石田洋一,菊池邦生,大口昭英,松原茂樹,鈴木光明,竹下俊行
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2012-04-13
• [Presentation] 絨毛外栄養膜細胞に発現しているCD44の機能解析:HTR8/SVneoを用いた浸潤能の検討 (2011)
  • Author(s): 菊池邦生
  • Organizer: 第26回日本生殖免疫学会
  • Place of Presentation: ウィンク愛知(愛知県)
  • Year and Date: 2011-12-03
• [Presentation] 瀧澤俊広絨毛外栄養膜細胞に発現しているCD44の機能解析:HTR8/SVneoを用いた浸潤能の検討 (2011)
  • Author(s): 高橋宏典,菊池邦生,松原茂樹,鈴木光明
  • Organizer: 第26回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-12-02
• [Presentation] エクソソームmicroRNAを介した胎盤-リンパ球間コミュニケーション:培養細胞を用いたモデル解析 (2011)
  • Author(s): アリモハメド,菊池邦生,石橋宰,石川朋子,瀧澤敬美,竹下俊行,瀧澤俊広
  • Organizer: 第26回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-12-02
• [Presentation] Placenta specific-microRNAs in normal pregnancy and preeclampsia. (2011)
  • Author(s): Toshihiro Takizawa, Md.Moksed Ali, Osamu Ishibashi, Kunio Kikuchi, Takuji Kosuge, Shigeki Matsubara, Toshiyuki Takeshita.
  • Organizer: 8th European Congress on Reproductive Immunology
  • Place of Presentation: ミュンヘン(ドイツ)
  • Year and Date: 2011-11-13
• [Presentation] 胎盤由来エクソソームを介した胎盤-母体間コミュニケーション (2011)
  • Author(s): 菊池邦生
  • Organizer: 第19回日本胎盤学会学術集会
  • Place of Presentation: 東京ステーションコンファレンス(東京都)(招待講演)
  • Year and Date: 2011-10-01
• [Presentation] 栄養膜浸潤における接着分子CD44の役割:浸潤能解析 (2011)
  • Author(s): 高橋宏典,菊池邦生,松原茂樹,鈴木光明,瀧澤俊広
  • Organizer: 第19回日本胎盤学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2011-09-30
• [Presentation] 胎盤由来エクソソームを介した胎盤-母体間コミュニケーション (2011)
  • Author(s): 菊池邦生,アリモハメド,羅善順,石橋宰,石川朋子,瀧澤敬美,倉品隆平,石川源,右田真,大口昭英,松原茂樹,竹下俊行,瀧澤俊広
  • Organizer: 第19回日本胎盤学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2011-09-30
• [Presentation] Placenta specific-microRNAs in normal pregnancy and preeclampsia (2010)
  • Author(s): Toshihiro Takizawa
  • Organizer: 8th European Congress on Reproductive Immunology
  • Place of Presentation: Munich, Germany
  • Year and Date: 2010-11-13
• [Presentation] microRNAの網羅的解析手法と生殖免疫学分野での応用
  • Author(s): 菊池 邦生
  • Organizer: 第２7回生殖免疫学会総会・学術集会
  • Place of Presentation: 大阪医科大学（大阪府）
  • Invited
• [Presentation] Methylation profile of C19MC-miRNA CpG island in trophoblast cell lines.
  • Author(s): Kunio Kikuchi
  • Organizer: 第18回国際胎盤学会
  • Place of Presentation: 広島国際会議場（広島県）
  • Invited
• [Presentation] MicroRNAによるGRB2の発現制御を介した肝内胆管癌の移動能制御機構の解析
  • Author(s): 菊池 邦生
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポートホール高松・かがわ国際会議場（香川県）
• [Book] 臨床婦人科産科 (2011)
  • Author(s): 菊池邦生、石橋宰、竹下俊行、瀧澤俊広