The maintenance of the IgE antibody secreting cells
| Project/Area Number |
22791572
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Chiba University |
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Principal Investigator |
INAMINE Ayako 千葉大学, 大学院・医学研究院, 特任研究員 (70466720)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 鼻科学 / 免疫学 / アレルギー・ぜんそく / 細胞・組織 / 発生・分化 / 遺伝子 |
|---|
| Research Abstract |
In seasonal allergy (such as Japanese cedar pollinosis), pollen specifi c IgE Ab production has been persistent even in the absent season of allergens. Since long-lived plasma cells (LL-PCs) in bone marrow (BM) are important for the maintenance of serum Ab titers, the persistent production of IgE Abs in Allergic patients may be due to IgE-LL-PCs in BM. However, IgE-LL-PCs in murine BMs have never been detected. Moreover, high concentration of IgE Abs was detected in serum of nasal polyposis patients and IgE-PCs were accumulated in the nasal polyposis tissue. In this study, we investigated the mechanism of IgE-LL-PCs generation using IL-21R-KO mice which have high concentration of serum IgE titers. When the number of IgE-PCs was examined in IL-21R-KO mice by ELISPOT assay, no IgE-PCs were detected in BM, despite of the high serum IgE concentration. Then, we have established the culture system to generate LL-PCs from naive B cells using IL-21, and those IgE-PCs were transferred into mice to examine the generation of IgE-LL-PCs in BMs. Since LL-PCs are derived from germinal center (GC) B cells was impaired in IL-21R-KO mice, we will discuss the role of IL-21 in the generation of LL-PCs including IgE-LL-PCs. These results suggested that IgE-LL-PCs would provide a new target for immunotherapy in Allergic diseases.
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Report
(3 results)
Research Products
(35 results)
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[Journal Article] Increase of regulatory T cells and the ratio of specific IgE to total IgE are candidates for response monitoring or prognostic biomarkers in two-year sublingual immunotherapy (SLIT) for Japanese cedar pollinosis2011
Author(s)
Fujimura, T., Yonekura, S., Horiguchi, S., Taniguchi, Y., Saito, A., Yasueda, H., Inamine, A., Nakayama, T., Takemori, T., Taniguchi, M., Sakaguchi, M., Okamoto, Y.
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Journal Title
Clin. Immunol
Volume: 139(1)
Pages: 65-74
Related Report
Peer Reviewed
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[Journal Article] CXCR4 expression on activated B cells is down-regulated by CD63 and IL-21.2011
Author(s)
Yoshida, N., Kitayama, D., Arima M., Sakamoto A., Inamine A., Takano, H., Hatano, M., Koike, T., Tokuhisa, T.
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Journal Title
J.Immunol.
Volume: 186(5)
Pages: 2800-2808
Related Report
Peer Reviewed
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[Journal Article] Increase of regulatory T cells and the ratio of specific IgE to total IgE are candidates for response monitoring or prognostic biomarkers in two-year sublingual immunotherapy (SLIT) for Japanese cedar pollinosis.2011
Author(s)
Fujimura, T., Yonekura, S., Horiguchi, S., Taniguchi, Y., Saito, A., Yasueda, H., Inamine, A., Nakayama, T., Takemori, T., Taniguchi, M., Sakaguchi, M., Okamoto, Y.
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Journal Title
Clin.Immunol.
Volume: 139(1)
Pages: 65-74
Related Report
Peer Reviewed
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[Patent(Industrial Property Rights)] 花粉症ワクチンの治療効果を予測するバイオマーカー2011
Inventor(s)
岡本美孝, 稲嶺絢子, 櫻井大樹, 中山俊憲, 小原収, 山下政克, 野中謙
Industrial Property Rights Holder
岡本美孝, 稲嶺絢子, 櫻井大樹, 中山俊憲, 小原収, 山下政克, 野中謙
Industrial Property Number
2011-076653
Filing Date
2011-03-30
Related Report
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