Research Project
Grant-in-Aid for Young Scientists (B)
In seasonal allergy (such as Japanese cedar pollinosis), pollen specifi c IgE Ab production has been persistent even in the absent season of allergens. Since long-lived plasma cells (LL-PCs) in bone marrow (BM) are important for the maintenance of serum Ab titers, the persistent production of IgE Abs in Allergic patients may be due to IgE-LL-PCs in BM. However, IgE-LL-PCs in murine BMs have never been detected. Moreover, high concentration of IgE Abs was detected in serum of nasal polyposis patients and IgE-PCs were accumulated in the nasal polyposis tissue. In this study, we investigated the mechanism of IgE-LL-PCs generation using IL-21R-KO mice which have high concentration of serum IgE titers. When the number of IgE-PCs was examined in IL-21R-KO mice by ELISPOT assay, no IgE-PCs were detected in BM, despite of the high serum IgE concentration. Then, we have established the culture system to generate LL-PCs from naive B cells using IL-21, and those IgE-PCs were transferred into mice to examine the generation of IgE-LL-PCs in BMs. Since LL-PCs are derived from germinal center (GC) B cells was impaired in IL-21R-KO mice, we will discuss the role of IL-21 in the generation of LL-PCs including IgE-LL-PCs. These results suggested that IgE-LL-PCs would provide a new target for immunotherapy in Allergic diseases.
All 2012 2011 2010
All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (21 results) Book (1 results) Patent(Industrial Property Rights) (3 results)
Clin Immunol
Volume: 143(2) Pages: 170-179
A preliminary prospective study
Volume: 26 Pages: 1-5
Am J Rhinol Allergy
Clinical Immunology
Volume: 143(2) Pages: 170-9
J. Immunol
Volume: 186 Pages: 2800-2808
Clin. Immunol
Volume: 139(1) Pages: 65-74
耳鼻咽喉科免疫アレルギー
Volume: 29(3) Pages: 215-220
10030646969
JOHNS
Volume: 27 Pages: 838-842
J.Immunol.
Volume: 186(5) Pages: 2800-2808
Clin.Immunol.
