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Basic research about allergic rhinitis with CpG-DNA

Research Project

Project/Area Number 22791581
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionUniversity of Fukui

Principal Investigator

KUBO Seita  福井大学, 医学部・附属病院, 助教 (80401983)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsCpG / B細胞 / PD-L1 / T細胞 / 共刺激分子 / B7RP-1 / CD30 / アレルギー性鼻炎
Research Abstract

Co-stimulatory molecules are important for regulating T cell activation and immune response. PD-L1 has emerged as an important immune modulator that can block T cell receptor signaling. We have investigated whether PD-L1 could be expressed in human B cells stimulated by CpG-DNA. CpG-DNA strongly induced the coinhibitory molecule ligand, PD-L1 of human B cells. Results show that nuclear factor-kappa B(NF-kB) signaling is involved directly in CpG-DNA-induced PD-L1 expression in human B cells. We sought to determine the effect of CpG-DNA-treated B cells on Th2 cytokine production in Cry j 1(Japanese pollen antigen)-stimulated human CD4-positive cells from patients with seasonal allergic rhinitis caused by Japanese cedar pollen. CpG-DNA-treated B cells reduced Cry j 1-induced IL-5 and IL-13 production in CD4-positive cells. When the binding of PD-1 to PD-L1 was inhibited by PD-1-Ig, this chimera-molecule reversed the previously described reductions in IL-5 and IL-13 production. These results indicate that CpG-DNA induces the coinhibitory molecule ligand PD-L1 expression in human B cells and PD-L1 can suppress Th2 cytokine production in Cry j 1-stimulated CD4-positive cells, while CpG-DNA increased Th1 cytokine production and reduced the expression of costimulatory molecule ligands that can promote Th2 inflammatory responses.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (7 results)

All 2012 2011 2010 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] CpG-DNA induces CD274-expression in human B cells and suppresses Th2 cytokine production in pollen antigen-stimulated CD4-positive cells2012

    • Author(s)
      窪誠太
    • Journal Title

      Clinical and Experimental Immunology

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CpG-DNA induces CD274-expression in human B cells and suppresses Th2 cytokine production in pollen antigen-stimulated CD4-positive cells

    • Author(s)
      Seita Kubo, Takechiyo Yamada, Yoko Osawa, Yumi Ito, Norihiko Narita and Shigeharu Fujieda
    • Journal Title

      Clinical and Experimental Immunology

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] CpG-ODN induces CD274 expression on human B cells and CpG ODN-treated B cells decreased IL-5 production from antigen-stimulated human CD4+cells2012

    • Organizer
      International Conference on Drug Discovery and Therapy
    • Place of Presentation
      Dubai
    • Year and Date
      2012-02-15
    • Related Report
      2011 Final Research Report
  • [Presentation] CpG-ODN induces CD274 expression on human B cells and CpG ODN-treated B cells decreased IL-5 production from antigen-stimulated human CD4+ cells2012

    • Author(s)
      窪誠太
    • Organizer
      International Conference on Drug Discovery and Therapy
    • Place of Presentation
      ドバイ、UAE(招待講演)
    • Year and Date
      2012-02-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] CpG-DNA induces CD274 expression on human B cells2011

    • Organizer
      International Symposium on Infection and Allergy of the Nose
    • Place of Presentation
      Tokyo
    • Year and Date
      2011-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] CpG-DNA induces CD274 expression on human B cells2011

    • Author(s)
      窪誠太
    • Organizer
      International Symposium on Infection and Allergy of the Nose
    • Place of Presentation
      東京
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] CpG-DNA induces PD-L1 (CD274) expression on human B cells2010

    • Author(s)
      窪誠太
    • Organizer
      International Symposium on Tonsils and Mucosal Barriers of the Upper Airways
    • Place of Presentation
      旭川市
    • Year and Date
      2010-07-07
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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