Comprehensive profiling of the gene expression in the inner ear sensory epithelium and its application to the regeneration

• Project/Area Number: 22791595
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Kyoto University
• Principal Investigator: YAMAMOTO Norio 京都大学, 医学研究科, 助教 (70378644)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 遺伝子発現解析 / 内耳 / Notchシグナル / Septin / プロスタグランジン / 内耳発生 / 単一細胞

Research Abstract

We performed preliminary experiments to set up the condition of extracting RNA from single cochlear cells and amplifying the cDNA linearly. In addition, we found the function of several genes in the cochlea. These results will contribute to the realization of cochlear regenerative medicine. Complete inhibition of Notch signaling in the mouse cochlear caused small numbers of inner hair cells only in the apical turn. This result indicates that Notch signaling is involved not only in the determination of cell fate but also in the maintenance of the cochlear sensory epithelial progenitors. The analysis of EP4 knockout mice revealed that prostaglandin E physiologically protects cochlear hair cells. We also found the new markers of cochlear supporting cells, Septin4, Septin5, and Septin7.

Research Products

• [Journal Article] Hear Res Localization of septin proteins in the mouse cochlea (2012)
  • Author(s): Yoshida A, Yamamoto N, Kinoshita M, Hiroi N, Hiramoto T, Kang G, Trimble WS, Tanigaki K, Nakagawa T, Ito J.
  • Journal Title: Epub ahead of print Volume: 289 Issue: 1-2 Pages: 40-51
  • DOI: 10.1016/j.heares.2012.04.015
  • Peer Reviewed
• [Journal Article] Role of PGE-type receptor 4 in auditory function and noise-induced hearing loss in mice (2012)
  • Author(s): Hamaguchi, K., Yamamoto, N., Nakagawa, T., Furuyashiki, T., Narumiya, S., Ito, J.
  • Journal Title: Neuropharmacology Volume: 62 Issue: 4 Pages: 1841-1847
  • DOI: 10.1016/j.neuropharm.2011.12.007
  • NAID: 120003874264
  • Peer Reviewed
• [Journal Article] A minimally invasive approach for cochlear implantation using a microendoscope (2012)
  • Author(s): Hiraumi H, Yamamoto N, Sakamoto T, Ito I
  • Journal Title: Eur Arch Otorhinolaryngol Volume: (Epub) Issue: 2 Pages: 477-481
  • DOI: 10.1007/s00405-012-2004-9
  • NAID: 120005477216
  • Peer Reviewed
• [Journal Article] Rbpj regulates development of prosensory cells in the mammalian innerear (2011)
  • Author(s): Yamamoto N, Chang W, Kelley MW
  • Journal Title: Dev Biol Volume: 353(2) Issue: 2 Pages: 367-379
  • DOI: 10.1016/j.ydbio.2011.03.016
  • Peer Reviewed
• [Journal Article] Management of labyrinthine fistulae in Kyoto University Hospital (2010)
  • Author(s): Yamamoto N, Fujimura S, Ogino E, Hiraumi H, Sakamoto T, Ito J
  • Journal Title: Acta Otolaryngol Suppl Volume: 563 Issue: sup563 Pages: 16-9
  • DOI: 10.3109/00016489.2010.489232
  • Peer Reviewed
• [Journal Article] Outcome of ossiculoplasty in Kyoto University Hospital (2010)
  • Author(s): Yamamoto N, Ogino E, Hiraumi H, Sakamoto T, Ito J
  • Journal Title: Acta Otolaryngol Suppl Volume: 563 Issue: sup563 Pages: 11-5
  • DOI: 10.3109/00016489.2010.489231
  • Peer Reviewed
• [Journal Article] Topicalinsulin-like growth factor 1 treatment using gelatin hydrogels for glucocorticoid-resistant sudden sensorineural hearing loss : a prospective clinical trial (2010)
  • Author(s): Nakagawa T, Sakamoto T, Hiraumi H, Kikkawa YS, Yamamoto N, Hamaguchi K, Ono K, Yamamoto M, Tabata Y, Teramukai S, Tanaka S, Tada H, Onodera R, Yonezawa A, Inui K, Ito J
  • Journal Title: BMC Med Volume: 25 Issue: 1
  • DOI: 10.1186/1741-7015-8-76
  • Peer Reviewed
• [Presentation] Protein Expression in the Embryonic and米国サンディエゴ、Septin Protein Expression in the Embryonic and Neonatal Mouse Cochlea (2012)
  • Author(s): Yamamoto, N, Yoshida, A, Nakagawa, T, Ito, J
  • Organizer: Thirty-Fifth Annual Mid Winter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: 米国サンディエゴ
  • Year and Date: 2012-02-28
• [Presentation] Septin Protein Expression in the Embryonic and Neonatal Mouse Cochlea (2012)
  • Author(s): Yamamoto, Norio; Yoshida, Atsuhiro; Nakagawa, Takayuki
  • Organizer: The Thirty-Fifth Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2012-02-28
• [Presentation] IGF-1による蝸牛保護メカニズムの解明 (2011)
  • Author(s): 山本典生; 林裕史; 中川隆之; 伊藤壽一
  • Organizer: 第21回日本耳科学会総会・学術講演会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2011-11-25
• [Presentation] Mechanisms of mammalian cochlear hair cell protection by IGF-1 (2011)
  • Author(s): Yamamoto, N, Hayashi, Y, Nakagawa T, Ito, J
  • Organizer: The 8th Asia Pacific Symposium on Cochlear Implant and Related Sciences
  • Place of Presentation: 韓国Daegue
  • Year and Date: 2011-10-26
• [Presentation] Mechanisms of mammalian cochlear hair cell protection by IGF-1 (2011)
  • Author(s): Yamamoto, Norio; Hayashi, Yushi; Takayuki, Nakagawa; Ito, Juici
  • Organizer: The 8th Asia Pacific Symposium on Cochlear Implant and Related Sciences
  • Place of Presentation: Daegu, Korea
  • Year and Date: 2011-10-26
• [Presentation] Parkin Deficiency Causes Progressive Hearing Loss in Mice through Outer Hair Cell Loss (2011)
  • Author(s): Yamamoto, N, Hamaguchi, K, Nakagawa, T, Ito, J
  • Organizer: 48th Inner Ear Biology Workshop
  • Place of Presentation: ポルトガル・リスボン
  • Year and Date: 2011-09-20
• [Presentation] Parkin Deficiency Causes Progressive Hearing Loss in Mice through Outer Hair Cell Loss (2011)
  • Author(s): Yamamoto, Norio; Hamaguchi, Kiyomi; Nakagawa, Takayuki; Ito, Juichi
  • Organizer: 48th Inner Ear Biology Workshop
  • Place of Presentation: Lisboa, Portgal
  • Year and Date: 2011-09-20
• [Presentation] ステロイド局所投与が著効した顎関節外耳道内突出例 (2011)
  • Author(s): 山本典生; 平海晴一; 坂本達則; 伊藤壽一
  • Organizer: 第73回耳鼻咽喉科臨床学会総会・学術講演会
  • Place of Presentation: 松本市
  • Year and Date: 2011-06-24
• [Presentation] Notchシグナルは内耳感覚上皮前駆細胞を維持する (2011)
  • Author(s): 山本典生;伊藤壽一
  • Organizer: 第112回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2011-05-19
• [Presentation] Notchシグナルは内耳感覚上皮前駆細胞を維持する (2011)
  • Author(s): 山本典生; 伊藤壽一
  • Organizer: 第112回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 京都市
  • Year and Date: 2011-05-19
• [Presentation] Parkin Deficiency Causes Progressive Hearing Loss in Mice Through Outer Hair Cell Loss (2011)
  • Author(s): Yamamoto N, Hamaguchi K, Nakagawa K, Ito J
  • Organizer: Thirty-Fourth Annual Mid Winter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: 米国ボルチモア
  • Year and Date: 2011-02-20
• [Presentation] Parkin Deficiency Causes Progressive Hearing Loss in Mice Through Outer Hair Cell Loss (2011)
  • Author(s): Yamamoto N, Hamaguchi K, Nakagawa K, Ito J
  • Organizer: Thirty-Fourth Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: ボルチモア・米国
  • Year and Date: 2011-02-20
• [Presentation] Rbpj is Required for Development of Prosensory Cells in the Mammalian Inner Ear (2010)
  • Author(s): Yamamoto N, Chang W, Kelley M
  • Organizer: Sixth International Symposium on Meniere's Disease and Inner Ear Disorders
  • Place of Presentation: 京都
  • Year and Date: 2010-11-17
• [Presentation] Parkinノックアウトマウスの聴覚に関する検討 (2010)
  • Author(s): 山本典生, 濱口清海, 中川隆之, 伊藤壽一
  • Organizer: 第20回日本耳科学会総会・学術講演会
  • Place of Presentation: 松山
  • Year and Date: 2010-10-07
• [Presentation] Cochlear implant cases with syndromic sensorineural hearing loss from mitochondrial diseases (2010)
  • Author(s): Yamamoto N, Okuyama H, Hiraumi H, Sakamoto T, Ito J
  • Organizer: The 13th Korea-Japan Joint Meeting of Otorhinolaryngology-Head and Neck Surgery
  • Place of Presentation: ソウル・韓国
  • Year and Date: 2010-09-10
• [Presentation] Expression Pattern of Olig Gene Family in the Developing Inner Ears (2010)
  • Author(s): Yamamoto N, Yoshida A, Nakagawa T, Ito J
  • Organizer: 47th Inner Ear Biology Workshop
  • Place of Presentation: チェコ共和国・プラハ
  • Year and Date: 2010-08-31
• [Presentation] Expression Pattern of Olig Gene Family in the Developing Inner Ears (2010)
  • Author(s): Yamamoto N, Yoshida A, Nakagawa T, Ito J
  • Organizer: 47th Inner Ear Biology Workshop
  • Place of Presentation: プラハ・チェコ共和国
  • Year and Date: 2010-08-31
• [Presentation] Cochlear implant cases with syndromic sensorineural hearing loss from mitochondrial diseases (2010)
  • Author(s): Yamamoto N, Okuyama H, Hiraumi H, Sakamoto T, Ito J
  • Organizer: 11th International Conference on Cochlear Implants and Other Implantable Auditory Technologies
  • Place of Presentation: ストックホルム・スウェーデン
  • Year and Date: 2010-07-01
• [Presentation] マウス蝸牛におけるSept4タンパク質発現および機能の解析 (2010)
  • Author(s): 山本典生, 吉田充裕, 中川隆之, 伊藤壽一
  • Organizer: 第111回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 仙台
  • Year and Date: 2010-05-20