Quantitative analysis and the investigation of the pneumococcal nasopharyngeal colonization in children.

• Project/Area Number: 22791623
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Wakayama Medical University
• Principal Investigator: OGAMI Masashi 和歌山県立医科大学, 医学部, 博士研究員 (90423946)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 鼻科学 / 肺炎球菌 / 肺炎球菌共通抗原 / イムノクロマトグラフィー法 / 鼻咽腔 / リアルタイムPCR

Research Abstract

Streptococcus pneumonia(S. pneumoniae) colonized in nasopharynx of children is an important pathogenic bacteria in pediatric acute otitis media and rhinosinusitis. In this study, we performed quantitative analysis of nasopharyngeal S. pneumonia by using real-time PCR method. In the healthy children, the concentration of S. pneumoniae showed an inverse relation with the age. On the other hands, the concentration of S. pneumoniae in the children with upper respiratory infection was higher than the healthy children. Moreover, we revealed that a new rapid antigen test for detection of pneumococci in middle ear fluids and nasopharyngeal secretions can be an available tool for detection S. pneumoniae.

Research Products

• [Journal Article] A Comparison of Conventional and Molecular Microbiology in Detecting Differences in Pneumococcal Colonization in Healthy Children and Children with Upper Respiratory Illness (2010)
  • Author(s): Ogami M, Hotomi M, Togawa A, Yamanaka N
  • Journal Title: Eur J pediatr Volume: 169(10) Pages: 1221-5
  • Peer Reviewed
• [Presentation] Quantitative serotype determination of nasopharyngeal carriage of Streptococcus pneumoniae (2010)
  • Author(s): Masashi Ogami, Muneki Hotomi, Noboru Yamanaka
  • Organizer: 10^<th> International Congress of the European Society of Pediatric Otorhinolaryngology
  • Place of Presentation: Pamplona、Spain
  • Year and Date: 2010-06-08
• [Presentation] Quantitative serotype determination of nasopharyngeal carriage of Strep tococcus pneumoniae (2010)
  • Author(s): Masashi Ogami, Muneki Hotomi, Noboru Yamanaka
  • Organizer: 10^<th> International Congress of the European Society of Pediatric Otorhinolaryngology
  • Place of Presentation: Pamplona, Spain
  • Year and Date: 2010-06-08
• [Presentation] 小児鼻咽腔細菌叢における肺炎球菌のダイナミズムに関する研究 (2010)
  • Author(s): 小上真史、保富宗城、戸川彰久、田村真司、山中昇
  • Organizer: 日本耳鼻咽喉科学会総会
  • Place of Presentation: 宮城県、日本
  • Year and Date: 2010-05-19
• [Book] JOHNS (2010)
  • Author(s): 小上真史、保富宗城、山中昇
  • Total Pages: 6
  • Publisher: 東京医学社
• [Book] JOHNS 特集 感染症-最近の話題 (2010)
  • Author(s): 小上真史、保富宗城、山中昇
  • Total Pages: 6
  • Publisher: 東京医学社