The role of novel extracellular matrix protein on CNS regeneration and its application study for glaucoma treatment

• Project/Area Number: 22791651
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Kanazawa University
• Principal Investigator: KORIYAMA Yoshiki 金沢大学, 医学系, 助教 (70397199)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 神経再生 / 視神経 / 網膜 / 接着因子 / ラミニン / 接着斑キナーゼ / Akt1 / 緑内障 / 神経生存 / プルプリン / 細胞外マトリックス

Research Abstract

In our strategies toward optic nerve regeneration of mammals using fish derived molecule, we used purpurin(a novel extracellular matrix protein) overexpression systems by adeno-associated virus vector in rat retina. In rat, supply of purpurin could protect retinal ganglion cells(RGCs) from injury-induced apoptosis through a mechanism of focal adhesion kinase(FAK)/Akt activation. Furthermore, purpurin had adhesive effect on rat RGCs from culture study with purpurin-coated dishes. We focused on the extracellular matrix-related molecules as a mechanism of purpurin adhesive and survival effects. Purpurin inhibited optic injury-induced matrix metalloprotease 9 activity and the degradation of laminin and activated FAK signaling. Purpurin could potentially offer new avenues for developing treatments for human retinal degenerative disorders such as glaucoma.

Research Products

• [Journal Article] NgR1 and NgR3 are receptors forchondroitin sulfate proteoglycans (2012)
  • Author(s): Dickendesher TL, Baldwin KT, Mironova YA, Koriyama Y, Raiker SJ, Askew KL, Wood A, Geoffroy CG, Zheng B, Liepmann CD, Katagiri Y, Benowitz LI, Geller HM, Giger RJ.
  • Journal Title: NatureNeurosci Volume: 15 Pages: 703-712
  • Peer Reviewed
• [Journal Article] NgR1 and NgR3 are receptors for chondroitin sulfate proteoglycans (2012)
  • Author(s): Dickendesher TL
  • Journal Title: Nat.Neurosci. Volume: (In press) Issue: 5 Pages: 703-712
  • DOI: 10.1038/nn.3070
  • Peer Reviewed
• [Journal Article] An application for mammalian optic nerve repair by fish regeneration-associated genes (2012)
  • Author(s): Koriyama Y
  • Journal Title: Adv Exp Med Biol. Volume: 723 Pages: 161-166
  • Peer Reviewed
• [Journal Article] Restoring visualcircuits and function after optic nerveinjury in adult mice (2011)
  • Author(s): Lima S, Koriyama Y, Kurimoto T, OliveiraJT, Yin Y, Li Y, Gilbert HY, Fagiolini M., Martinez AM, Benowitz L
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] Neuritogenic activity of a genipin derivative in retinal ganglion cells is mediated by retinoic acid receptorβexpression through nitric oxide/S-nitrosylationsignaling (2011)
  • Author(s): Koriyama Y, Takagi Y, Chiba K, YamazakiM, Arai K, Matsukawa T, Suzuki H, SugitaniK, Kagechika H, Kato S
  • Journal Title: J. Neurochem Volume: 119 Pages: 1232-1242
  • Peer Reviewed
• [Journal Article] Neuritogenic activity of a genipin derivative in retinal ganglion cells is mediated by retinoic acid receptor beta expression through nitric oxide/S-nitrosylation signaling (2011)
  • Author(s): Koriyama Y, Takagi Y, Chiba K, Yamazaki M, Arai K, Matsukawa T, Suzuki H, Sugitani K, Kagechika H, Kato S
  • Journal Title: J. Neurochem. Volume: 119 Issue: 6 Pages: 1232-1242
  • DOI: 10.1111/j.1471-4159.2011.07533.x
  • Peer Reviewed
• [Journal Article] An application for mammalian optic nerve repair by goldfish regeneration associaled genes (2011)
  • Author(s): Yoshiki Koriyama
  • Journal Title: Adv Exp Med Biol Volume: (In press)
  • Peer Reviewed
• [Journal Article] 5-S-GAD Attenuates Fe (2+)-induced lipid peroxidation and cell death in a neuronal cell model. (2011)
  • Author(s): Tsuneo Takadera
  • Journal Title: Neurotox Res Volume: (In press)
  • Peer Reviewed
• [Journal Article] Long-acting genipin derivative protects retinal ganglion cells from oxidative stress models in vitro and in vivo through the Nrf2/antioxidant response element signaling pathway (2010)
  • Author(s): Koriyama Y, Chiba K, Yamazaki M, SuzukiH, Muramoto K, Kato S
  • Journal Title: J. Neurochem Volume: 115 Pages: 79-91
  • NAID: 120002561626
  • Peer Reviewed
• [Journal Article] Long-acting genipin derivative protects retinal ganglion cells from oxidative stress models in vitro and in vivo through the Nrf2/ARE signaling pathway. (2010)
  • Author(s): Yoshiki Koriyama
  • Journal Title: J.Neurochem Volume: 115 Pages: 79-91
  • Peer Reviewed
• [Journal Article] A hypoplastic retinal lamination in the purpurin knock down embryo in zebrafish (2010)
  • Author(s): Mikiko Nagashima
  • Journal Title: Adv Exp Med Biol Volume: 664 Pages: 517-524
  • Peer Reviewed
• [Presentation] NgR1 andNgR3 are inhibitory receptors forchondroitin sulfate proteoglycans (2011)
  • Author(s): TL. Dickendesher, KT. Baldwin, YA. Mironova, Y. Koriyama, SJ. Raiker, Y. Duan, Y. Katagiri, CG. Geoffroy, KL. Askew, B. Bates, MM. Zaleska, A. Wood, B. Zheng, LI. Benowitz, HM. Geller, RJ. Roman
  • Organizer: Society for Neuroscience 41th AnnualMeeting
  • Place of Presentation: ワシントンコンベンションセンター(アメリカ)
  • Year and Date: 2011-11-13
• [Presentation] NgR1 and NgR3 are inhibitory receptors for chondroitin sulfate proteoglycans (2011)
  • Author(s): Dickendesher TL
  • Organizer: Society for Neuroscience 41th Annual Meeting
  • Place of Presentation: ワシントンコンベンションセンター(アメリカ)
  • Year and Date: 2011-11-13
• [Presentation] 視神経損傷後の新規細胞外マトリックス分子プルプリンによるラット網膜神経節細胞の生存と軸索伸長 (2011)
  • Author(s): 郡山恵樹、松川通、永島幹子、加藤聖
  • Organizer: 第54回日本神経化学会
  • Place of Presentation: 瑠璃光(石川県)
  • Year and Date: 2011-09-28
• [Presentation] 視神経損傷後の新規細胞外マトリックス分子プルプリンによるラット網膜神経節細胞の生存と軸索伸長 (2011)
  • Author(s): 郡山恵樹
  • Organizer: 第54回日本神経化学会
  • Place of Presentation: 瑠璃光(石川)
  • Year and Date: 2011-09-28
• [Presentation] 一酸化窒素放出性IPRG001による網膜神経節細胞の保護作用と視神経再生 (2011)
  • Author(s): 郡山恵樹
  • Organizer: 第26回神経組織の成長・再生・移植研究会
  • Place of Presentation: 東京医科大(東京)
  • Year and Date: 2011-06-25
• [Presentation] 合成レチノイドAm80による中枢神経保護・再生作用 (2010)
  • Author(s): 郡山恵樹
  • Organizer: 第21回日本レチノイド研究会学術集会
  • Place of Presentation: 大阪医科大学(大阪)
  • Year and Date: 2010-11-14
• [Presentation] A novel extracellular matrixstabilizing molecule (2010)
  • Author(s): Y. Koriyama, K. Sugitani, T. Matsukawa, S. Kato
  • Organizer: The XIVth international symposium on retinal degenerations
  • Place of Presentation: トレンブラントホテル(カナダ)
  • Year and Date: 2010-07-14
• [Presentation] 魚類神経再生分子を用いたほ乳類中枢神経修復への応用 (2010)
  • Author(s): 郡山恵樹
  • Organizer: 金沢大学十全医学会総会・学術集会
  • Place of Presentation: 金沢大学(石川)
  • Year and Date: 2010-07-09
• [Presentation] 魚類神経再生モデルを用いたほ乳類中枢神経修復・再生へのアプローチ (2010)
  • Author(s): 郡山恵樹、杉谷加代、松川通、加藤聖
  • Organizer: 第25回神経組織の成長・再生・移植研究会
  • Place of Presentation: 大阪医科大学(大阪府)
  • Year and Date: 2010-05-22
• [Presentation] 魚類神経再生モデルを用いたほ乳類中枢神経修復・再生へのアプローチ (2010)
  • Author(s): 郡山恵樹
  • Organizer: 第25回神経組織の成長・再生・移植研究会
  • Place of Presentation: 大阪市立大学(大阪)
  • Year and Date: 2010-05-22
• [Presentation] A novel extracellular matrix stabilizing molecule, purpurin protects injury-induced apoptosis and regenerate axons in rat retinal ganglion cells (2010)
  • Author(s): Yoshiki Koriyama
  • Organizer: The XIVth international symposium on retinal degenerations
  • Place of Presentation: Fairmont Tremblant(カナダ)