The elucidation of the role of ABC transporters in retinal angiogenesis
| Project/Area Number |
22791660
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Kobe University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ABCトランスポーター / MRP4 / 血管新生 / 網膜血管内皮細胞 / RNA干渉 / ノックアウトマウス / フォルスコリン |
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| Research Abstract |
Multidrug resistance protein 4(MRP4) is a membrane protein that belongs to ABC transporter superfamily. In vitro analysis using human retinal microvascular endothelial cells(HRECs) showed that MRP4 knockdown enhanced cell migration, suppressed apoptosis. Moreover, MRP4 knockdown HRECs assembled and aggregated into a massive tube-like structure in a Matrigel-based tube formation assay. Functional analysis using Mrp4 knockout mice demonstrated abnormal increase in capillary density in developing retina after intraperitoneal injections of forskolin which increase the intracellular level of cAMP, an important substrate for Mrp4. These results suggest that MRP4 has anti-angiogenic properties in retinal angiogenesis.
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Report
(3 results)
Research Products
(6 results)
