Suppression of inflammation with the cornea infection by the control of the natural immunity

• Project/Area Number: 22791666
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Kochi University
• Principal Investigator: SUMI Tamaki 高知大学, 教育研究部・医療学系, 助教 (70385243)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 角膜感染症 / 緑膿菌 / 角膜炎 / サイトカイン / 好中球 / 免疫学 / 眼炎症

Research Abstract

Keratitis was induced by the application of LPS in C57BL/ 6 mice. Time-points of infiltration into cornea differed between macrophages and neutrophils. Similarly, humoral factors such as cytokines and chemokines were detected at different time-point. I made a mouse model of Pseudomonas aeruginosa(P. aeruginosa.) keratitis. Severe keratitis was induced by 5x10^6 of CFU P. aeruginosa.(clinical separation). The average clinical score of keratitis was lower in the BALB/ c than C57BL/ 6 mice.

Research Products

• [Journal Article] B and T lymphocyte attenuator regulates the development of antigen induced experimental conjunctivitis (2012)
  • Author(s): Ishida W, Fukuda K, Kajisako M, Sumi T, Matsuda H, Yagita H, Fukushima A
  • Journal Title: Graefe' s Arch Clin Exp Ophthalmol Volume: Vol.250 Pages: 289-295
  • Peer Reviewed
• [Journal Article] アレルギー薬によるマスト細胞からのメディエーター遊離抑制効果の比較 (2012)
  • Author(s): 角環, 山田美絵, 高橋祐次, 今井俊祐, 葛西洋芳, 石田わか, 福島敦樹
  • Journal Title: あたらしい眼科 Volume: 29巻 Pages: 103-108
  • Peer Reviewed
• [Journal Article] Automated hyperemia analysis software reliability and reproducibility in healthy subjects (2012)
  • Author(s): Yoneda T, Sumi T, Takahashi A, Hoshikawa Y, Kobayashi M, Fukushima A
  • Journal Title: Jpn J Ophthalmol Volume: Vol.56, No.1 Pages: 1-7
  • NAID: 10030248195
  • Peer Reviewed
• [Journal Article] Adjuvants determine the contribution of basophils to antigen sensitization in vivo (2011)
  • Author(s): Ishida W, Fukuda K, Sumi T, Ebihara N, Kajisako M, Matsuda H, Yagita H, Fukushima A
  • Journal Title: Immunol Lett Volume: Vol.136 Pages: 49-54
  • Peer Reviewed
• [Journal Article] Adjuvants determine the contribution of basophils to antigen sensitization in vivo. (2011)
  • Author(s): Ishida W, Fukuda K, Sumi T, Ebihara N, Kajisako M, Matsuda H, Yagita H, Fukushima A
  • Journal Title: Immunol Lett Volume: 136 Pages: 49-54
  • Peer Reviewed
• [Journal Article] Participation of CD11b and F4/ 80 molecules in the conjunctival eosinophilia of experimental allergic conjunctivitis (2010)
  • Author(s): Fukushima A, Ishida W, Ojima A, Kajisako M, Sumi T, Yamada J, Tsuru E, Miyazaki J-i, Tominaga A, Yagita H
  • Journal Title: Int Arch Allergy Immunol Volume: Vol.151 Pages: 129-136
  • Peer Reviewed
• [Journal Article] T cell-related co-stimulatory molecules in the conjunctiva of patients with severe allergic conjunctivitis (2010)
  • Author(s): Sumi T, Ishida W. Ebihara N, Fukushima A
  • Journal Title: Cornea Volume: Vol.29 Pages: 622-627
  • Peer Reviewed
• [Journal Article] Aggravation of conjunctival early-phase reaction by staphylococcus aureus enterotoxin B via augmentation of IgE production (2010)
  • Author(s): Miyazaki D, Ishida W, Tominaga T, Sumi T, Fukushima A
  • Journal Title: Jpn J Ophthalmol Volume: Vol.54, No.5 Pages: 476-480
  • Peer Reviewed
• [Journal Article] Evaluation of histamine-induced conjunctival edema in guinea-pigs using image analysis (2010)
  • Author(s): Takahashi A, Sumi T, Tada K, Mibu, Shii D, Kayasuga A, Fukushima A
  • Journal Title: Br J Ophthalmol Volume: Vol.94, No.12 Pages: 1657-1661
  • Peer Reviewed
• [Journal Article] Conjunctival macrophages act as antigen-presenting cells in the conjunctiva during the development of experimental allergic conjunctivitis (2010)
  • Author(s): Ishida W, Kajisako M, Takahashi A, Sumi T, van Rooijen N, Fukuda K, Fukushima A
  • Journal Title: Mol Vision Volume: Vol.16 Pages: 1280-1285
  • Peer Reviewed
• [Journal Article] 眼瞼と眼球結膜に限局したアミロイドーシスの1例 (2010)
  • Author(s): 中平麻美, 角環, 多田憲太郎, 佐々木香る, 井口みつこ, 弘井誠, 刑部安弘, 福島敦樹
  • Journal Title: 臨床眼科 Volume: 64巻, 8号 Pages: 1331-1335
  • Peer Reviewed
• [Journal Article] アレルギー性結膜疾患発症に関与する細胞と分子 (2010)
  • Author(s): 角環
  • Journal Title: 日本眼炎症学会誌 Volume: 12巻 Pages: 16-19
  • Peer Reviewed
• [Journal Article] T cell-related co-stimulatory molecules in the conjunctiva of patients with severe allergic conjunctivitis. (2010)
  • Author(s): Sumi T, Ishida W., Ebihara N, Fukushima A
  • Journal Title: Cornea Volume: 29 Pages: 622-627
  • Peer Reviewed
• [Journal Article] Aggravation of conjunctival early-phase reaction by staphylococcus aureus enterotoxin B via augmentation of IgE production. (2010)
  • Author(s): Miyazaki D, Ishida W, Tominaga T, Sumi T, Fukushima A
  • Journal Title: Jpn J Ophthalmol Volume: 54(5) Pages: 476-480
  • Peer Reviewed
• [Journal Article] Evaluation of histamine-induced conjunctival edema in guinea-pigs using image analysis. (2010)
  • Author(s): Takahashi A, Sumi T, Tada K, Mibu, Shii D, Kayasuga A, Fukushima A
  • Journal Title: Br J Ophthalmol Volume: 94(12) Pages: 1657-1661
  • Peer Reviewed
• [Journal Article] Conjunctival macrophages act as antigen-presenting cells in the conjunctiva during the development of experimental allergic conjunctivitis. (2010)
  • Author(s): Ishida W, Kajisako M, Takahashi A, Sumi T, van Rooijen N, Fukuda K, Fukushima A
  • Journal Title: Mol Vision Volume: 16 Pages: 1280-1285
  • Peer Reviewed
• [Presentation] Sporothrix schenckiiによる眼瞼炎と強膜膿瘍を認めた一例 (2012)
  • Author(s): 角環、福田憲、笠井真央、樽谷勝仁、永野弓枝、佐野栄紀、福島敦樹
  • Organizer: 角膜カンファランス
  • Place of Presentation: 東京
  • Year and Date: 2012-02-24
• [Presentation] アンケート調査による結膜充血の認識について (2012)
  • Author(s): 米田剛、角環、小松あゆみ、高橋亜矢子、福田憲、福島敦樹
  • Organizer: 第36回日本角膜学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-02-24
• [Presentation] 結膜アレルギーにおけるヒスタミンの関与:他覚所見の評価系、ならびにヒスタミンH_1受容体発現の評価系の確率 (2011)
  • Author(s): 福島敦樹、石田わか、角環、福田憲、椎大介、今井順也
  • Organizer: 第15回日本ヒスタミン学会
  • Place of Presentation: 岩手
  • Year and Date: 2011-10-21
• [Presentation] 画像診断解析による眼球結膜充血の客観的評価 (2011)
  • Author(s): 角環
  • Organizer: 第65回日本臨床眼科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-07
• [Presentation] 涙嚢炎を合併した眼類天疱瘡の一例 (2011)
  • Author(s): 三浦悠作、角環、宮崎千歌、福島敦樹
  • Organizer: 第65回日本臨床眼科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-07
• [Presentation] 解析ソフトを用いた眼球結膜充血の定量化 (2011)
  • Author(s): 角環、米田剛、高橋亜矢子、星川靖裕、小林正彦、福島敦樹
  • Organizer: 第23回日本アレルギー学会春季臨床大会
  • Place of Presentation: 千葉
  • Year and Date: 2011-05-14
• [Presentation] 各種抗アレルギー薬によるメディエーター遊離抑制作用の検討 (2011)
  • Author(s): 角環、山田美絵、高橋佑次、今井俊佑、葛西洋芳、福島敦樹
  • Organizer: 第35回日本角膜学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-02-18
• [Presentation] Image analyses of conjunctival hyperemia in humans (2010)
  • Author(s): Tamaki Sumi, Tsuyoshi Yoneda, Ayako Takahashi, Kentaro Tada, Yasuhiro Hoshikawa, Masahiko Kobayashi, Ken Fukuda, Atsuki Fukushima
  • Organizer: The 2^<nd> Asia Cornea Society Biennial Scientific Meeting
  • Place of Presentation: Kyoto
  • Year and Date: 2010-12-02
• [Presentation] マウスアレルギー性結膜炎モデルにおける抗原提示細胞の解析 (2010)
  • Author(s): 石田わか、高橋亜矢子、角環、福田憲、福島敦樹
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-25
• [Presentation] アレルギー性結膜疾患の疾患特異的QOL調査票の確率 (2010)
  • Author(s): 深川和己、藤島浩、福島敦樹、角環、他
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-11-11
• [Presentation] 実験的アレルギー性結膜炎発症におけるFcεRIα陽性細胞の果たす役割 (2010)
  • Author(s): 福田憲、石田わか、角環、海老原伸之、八木田秀雄、福島敦樹
  • Organizer: 第44回日本眼炎症学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-09
• [Presentation] 眼球結膜充血を定量的に評価するソフト (2010)
  • Author(s): 高橋亜矢子、角環、多田憲太郎、星川靖裕、小林正彦、福島敦樹
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-15