Search for the mechanism of dry eye disease using active oxygen excessive model mouse

• Project/Area Number: 22791692
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Keio University
• Principal Investigator: UCHINO Yuichi 慶應義塾大学, 医学部, 特任助教(非常勤) (80365337)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 活性酸素 / ドライアイ / 酸化傷害 / ミトコンドリア / 涙腺 / 炎症 / 分泌減少

Research Abstract

Tet-mev-1 mice revealed decreased tear production with the morphological changes. Tear quantity values in Tet-mev-1 mice were lower compared to the wild type mice. The lacrimal gland of Tet-mev-1 mice overproduced superoxide anion(O & _2^-) and oxidative protein compared to the wild type mice. Histopathological analyses showed the hallmarks of lacrimal gland inflammation by presence of intense mononuclear leukocytic infiltration and fibrosis around acinar cell staining by 8-OHdG antibody in the lacrimal gland of Tet-mev-1 mice. These findings strongly suggest that oxidative stress can be a causative factor for the development of dry eye disease.

Research Products

• [Presentation] A new mouse model of dry eye disease <Oxidative stress affect functional decline in lacrimal gland> (2011)
  • Author(s): 内野裕一
  • Organizer: Kyoto Cornea Club
  • Place of Presentation: ウェスチン都ホテル(京都府)
  • Year and Date: 2011-12-03
• [Presentation] ミトコンドリアとドライアイ (2011)
  • Author(s): 内野裕一、川北哲也、宮沢正樹、尾内宏美、安田佳代、石井恭正、小川葉子、榛村重人、石井直明、坪田一男
  • Organizer: 第16回シェーグレン症候群セミナー
  • Place of Presentation: 東京
  • Year and Date: 2011-06-04
• [Presentation] ミトコンドリアとドライアイ (2011)
  • Author(s): 内野裕一
  • Organizer: 第16回シェーグレン症候群セミナー
  • Place of Presentation: HAKUYOHビル(東京都)(招待講演)
  • Year and Date: 2011-06-04
• [Presentation] New model mouse of dry eye disease : Oxidative Stress Affect Functional Decline In Lacrimal Gland (2011)
  • Author(s): Yuichi Uchino
  • Place of Presentation: Kyoto Cornea Club,京都
• [Presentation] The role of intracellular oxidative stress in the mechanism of the dry eye disease (2011)
  • Author(s): Yuichi Uchino, Tetsuya Kawakita, Masaki Miyazawa, Takamasa Ishii, Hiromi Onouchi, Kayo Yasuda, Yoko Ogawa, Shigeto Shimmura, Naoaki Ishii, Kazuo Tsubota
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO)
  • Place of Presentation: Fort Lauderdale
• [Presentation] Tet-mev-1マウスを用いた細胞内酸化ストレスによるドライアイ発症メカニズムの解析 (2011)
  • Author(s): 内野裕一、川北哲也、宮沢正樹、尾内宏美、安田佳代、石井恭正、小川葉子、榛村重人、石井直明、坪田一男
  • Organizer: 第35回角膜カンファランス
  • Place of Presentation: 品川
• [Presentation] The role of intracellular oxidative stress in the mechanism of the dry eye disease (2011)
  • Author(s): 内野裕一
  • Organizer: Association for Research in Vision and Ophthalmology (ARVO) 2011
  • Place of Presentation: Fort Lauderdale, US
• [Presentation] Tet-mev-1マウスを用いた細胞内酸化ストレスによるドライアイ発症メカニズムの解析 (2011)
  • Author(s): 内野裕一
  • Organizer: 角膜カンファランス2011
  • Place of Presentation: 東京
• [Presentation] A New Mouse Model of Dry Eye Disease(Tet-mev-1 Mice): Oxidative Stress Affect Functional Decline in Lacrimal Gland (2010)
  • Author(s): Yuichi Uchino, Tetsuya Kawakita, Masaki. Miyazawa, Takamasa Ishii, Hiromi Onouchi, Kayo Yasuda, Shigeto Shimmura, Naoaki Ishii, Kazuo Tsubota
  • Organizer: The 6th International Conference on the Tear Film & Ocular Surface
  • Place of Presentation: Florence, Italy
• [Presentation] A NEW MOUSE MODEL OF DRY EYE DISEASE (Tet-mev-1 Mice) : OXIDATIVE STRESS AFFECT FUNCTIONAL DECLINE IN LACRIMAL GLAND. (2010)
  • Author(s): 内野裕一
  • Organizer: The 6th International Conference on the Tear Film & Ocular Surface
  • Place of Presentation: Florence, Italy