The utility of the Hh protein as the new serum tumor marker in the childhood solid cancer

• Project/Area Number: 22791710
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatric surgery
• Research Institution: Kyushu University
• Principal Investigator: SOUZAKI Ryota 九州大学, 大学病院, 助教 (10403990)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 神経芽腫 / hedgehog signal / 血清腫瘍マーカー / Hhシグナル / Hedgehog signal

Research Abstract

The hedgehog(Hh) signaling pathway plays an important role in the growth and patterning in a variety of tissues during embryonic development. On the other hand, the activation of the Hh signal pathway due to mutations or ligand-dependent stimulation of the pathway is associated with tumorigenesis of a lot of cancers. This study revealed that 67-79% neuroblastoma samples showed positive for Hh proteins. There was a significant association between Oh signal proteins and the status of MYCN gene. The percentage of Oh protein positive cells in early stage samples was higher than that with advanced stage. The 5-year OS rate of Hh protein positive cases without MYCN amplification is significantly higher than that inGLI1 negative cases. Based on these result, the Hh signal proteins are possible to be a serum marker of neuroblastoma.

Research Products

• [Journal Article] Implications of surgical intervention in the treatment of neuroblastomas : 20-year experience of a single institution (2012)
  • Author(s): Tajiri T, Souzaki R, Kinoshita Y, Koga Y, Suminoe A, Hara T, Taguchi T
  • Journal Title: Surg Today Volume: 42(3) Pages: 220-4
  • NAID: 10030231044
• [Journal Article] Augmented reality navigation system for laparoscopic splenectomy in children based on preoperative CT image using optical tracking device (2012)
  • Author(s): Ieiri S, Uemura M, Konishi K, Souzaki R, Nagao Y, Tsutsumi N, Akahoshi T, Ohuchida K, Ohdaira T, Tomikawa M, Tanoue K, Hashizume M, Taguchi T
  • Journal Title: Pediatr Surg Int Volume: 28(4) Pages: 341-6
• [Journal Article] Surgical intervention strategies for pediatric ovarian tumors : experience with 60 cases at one institution (2012)
  • Author(s): Tajiri T, Souzaki R, Kinoshita Y, Yosue R, Kohashi K, Oda Y, Taguchi T
  • Journal Title: Pediatr Surg Int Volume: 28(1) Pages: 27-31
• [Journal Article] Surgical intervention strategies for pediatric ovarian tumors : experience with 60 cases at one institution (2012)
  • Author(s): Tajiri T, Souzaki R, Kinoshita Y, et al
  • Journal Title: Pediatr Surg Int Volume: 28 Pages: 27-31
  • Peer Reviewed
• [Journal Article] Correlation between the number of segmental chromosome aberrations and the age at diagnosis of diploid neuroblastomas without MYCN amplification (2011)
  • Author(s): Souzaki R, Tajiri T, Teshiba R, Kinoshita Y, Yosue R, Kohashi K, Oda Y, Taguchi T
  • Journal Title: Pediatr Surg Volume: 46(12) Pages: 2228-32
• [Journal Article] Successful resection of an undifferentiated sarcoma in a child using a real-time surgical navigation system in an open magnetic resonance imaging operation room (2011)
  • Author(s): Souzaki R, Kinoshita Y, Matsuura T, Tajiri T, Taguchi T, Ieiri S, Hong J, Uemura M, Konishi K, Tomikawa M, Tanoue K, Hashizume M, Koga Y, Suminoe A, Hara T, Kohashi K, Oda Y
  • Journal Title: J Pediatr Surg Volume: 46(3) Pages: 608-11
• [Journal Article] High EGFR mRNA expression is a prognostic factor for reduced survival in pancreatic cancer after gemcitabine-based adjuvant chemotherapy (2011)
  • Author(s): Fujita H, Ohuchida K, Mizumoto K, Itaba S, Ito T, Nakata K, Yu J, Kayashima T, Hayashi A, Souzaki R, Tajiri T, Onimaru M, Manabe T, Ohtsuka T, Tanaka M
  • Journal Title: Int J Oncol Volume: 38(3) Pages: 629-41
• [Journal Article] Thegenetic and clinical significance of MYCN gain as detected by FISH in neuroblastoma (2011)
  • Author(s): Souzaki R, Tajiri T, Teshiba R, Higashi M, Kinoshita Y, Tanaka S, Taguchi T
  • Journal Title: Pediatr Surg Int Volume: 27(3) Pages: 231-6
• [Journal Article] EGFR tyrosine kinase inhibition worsens acute lung injury in mice with repairing airway epithelium (2011)
  • Author(s): Harada C, Kawaguchi T, OgataSuetsugu S, Yamada M, Hamada N, Maeyama T, Souzaki R, Tajiri T, Taguchi T, Kuwano K, Nakanishi Y
  • Journal Title: Am J Respir Crit Care Med Volume: 183(6) Pages: 743-51
• [Journal Article] Successful resection of an undifferentiated sarcoma in a child using a real-time surgical navigation system in an open magnetic resonance imaging operation room (2011)
  • Author(s): Souzaki R, Kinoshita Y, Matsuura T, Tajiri T, Taguchi T, et al
  • Journal Title: J Pediatr Surg Volume: 46 Pages: 608-611
  • Peer Reviewed
• [Journal Article] Correlation between the number of segmental chromosome aberrations and the age at diagnosis of diploid neuroblastomas without MYCN amplification (2011)
  • Author(s): Souzaki R, Tajiri T, Teshiba R, Kinoshita Y, Yosue R, Kohashi K, Oda Y, Taguchi T
  • Journal Title: J Pediatr Surg Volume: 46 Pages: 2228-2232
  • Peer Reviewed
• [Journal Article] Successful resection of an undifferentiated sarcoma in a child using a real-time surgical navigation system in an open magnetic resonance imaging operation room. (2011)
  • Author(s): Souzaki R, et al
  • Journal Title: J Pediatr Surg Volume: 46(3) Pages: 608-611
  • Peer Reviewed
• [Journal Article] The genetic and clinical significance of MYCN gain as detected by FISH in neuroblastoma. (2011)
  • Author(s): Souzaki R, et al
  • Journal Title: Pediatr Surg Int Volume: 27(3) Pages: 231-236
  • Peer Reviewed
• [Journal Article] Concordance for neuroblastoma in monozygotic twins : case report and review of the literature (2010)
  • Author(s): Tajiri T, Souzaki R, Kinoshita Y, Tanaka S, Koga Y, Suminoe A, Jiara T, Kohashi K, Oda Y, Masumoto K, Ohira M, Nakagawara A, Taguchi T
  • Journal Title: J Pediatr Surg Volume: 45(12) Pages: 2312-6
• [Journal Article] Hedgehog signaling pathway in neuroblastoma differentiation (2010)
  • Author(s): Souzaki R, Tajiri T, Souzaki M, Kinoshita Y, Tanaka S, Kohashi K, Oda Y, Katano M, Taguchi T
  • Journal Title: J Pediatr Surg Volume: 45(12) Pages: 2299-304
• [Journal Article] Gene expression levels as predictive markers of outcome in pancreatic cancer after gemcitabine-based adjuvant chemotherapy (2010)
  • Author(s): FujitaH, Ohuchida K, Mizumoto K, Itaba S, Ito T, Nakata K, Yu J, Kayashima T, Souzaki R, Tajiri T, Manabe T, Ohtsuka T, Tanaka M
  • Journal Title: Neoplasia Volume: 12(10) Pages: 807-17
• [Journal Article] Successful treatment of advanced pancreatoblastoma by a pyloruspreserving pancreatoduodenectomy after radiation and high-dose chemotherapy (2010)
  • Author(s): ouzaki R, Tajiri T, Kinoshita Y, Tanaka S, Koga Y, Suminoe A, Hara T, Kohashi K, Oda Y, Taguchi T
  • Journal Title: Pediatr Surg Int Volume: 26(10) Pages: 1045-8
• [Journal Article] Hedgehog signaling pathway in neuroblastoma differentiation. (2010)
  • Author(s): Souzaki R, et al
  • Journal Title: J Pediatr Surg Volume: 45(12) Pages: 2299-2304
  • Peer Reviewed
• [Presentation] 小児内視鏡外科手術における客観的技術評価システム開発の試み一小児内視鏡外科手術の安全性を求めて一 (2011)
  • Author(s): 宗崎良太、家入里志、石井裕之、富川盛雅、松岡紀之、高西淳夫、橋爪誠、田口智章
  • Organizer: 第21回九州内視鏡下外科手術研究会
  • Place of Presentation: 福岡
  • Year and Date: 2011-09-03
• [Presentation] Interval appendectomyの至適待機期間について (2011)
  • Author(s): 宗崎良太、家入里志、松浦俊治、永田公二、林田真、木ド義晶、田尻達郎、田口智章
  • Organizer: 第41回九州小児外科研究会
  • Place of Presentation: 福岡
  • Year and Date: 2011-08-27
• [Presentation] 精巣原発悪性腫瘍の2例 (2011)
  • Author(s): 宗崎良太、田尻達郎、代居良太、木下義晶、古賀友紀、住江愛子、孝橋賢・、小田義直、原寿郎、田口智章
  • Organizer: 第40回九州地区小児固形悪性腫瘍研究会
  • Place of Presentation: 福岡
  • Year and Date: 2011-02-19
• [Presentation] Correlation between the number of segmental chromosome aberrations and the age at diagnosis in diploid neuroblastomas without MYCN amplification (2011)
  • Author(s): Souzaki R, Tajiri T, Kinoshita Y, Yosue R, Kohashi K, Oda Y, Taguchi T
  • Organizer: PAPS2011
  • Place of Presentation: Mexico
• [Presentation] 小児外科領域における手術ナビゲーションの導入・実践 (2011)
  • Author(s): 宗崎良太、家入里志、田尻達郎、植村宗則、木下義晶、古賀友紀、住江愛子、孝橋賢一、小田義直、原寿郎、橋爪誠、田口智章
  • Organizer: 第111回日本外科学会定期学術集会
  • Place of Presentation: 東京
• [Presentation] 小児消化管穿孔に対して腹腔鏡下閉鎖術を行った2例 (2011)
  • Author(s): 宗崎良太、家入里志、橋爪誠、田口智章
  • Organizer: 第25回日本小児救急医学会
  • Place of Presentation: 東京
• [Presentation] 胎児仙尾部奇形腫の周術期合併症および後遺症に関する検討:本邦における他施設共同研究(第4報) (2011)
  • Author(s): 宗崎良太、臼井規朗、左勝則、佐合治彦、野坂俊介、中村知夫、金森豊、米田光宏、北野良博、田口智章
  • Organizer: 第47回日本周産期・新生児学会学術集会
  • Place of Presentation: 札幌
• [Presentation] 精巣原発悪性腫瘍の2例 (2011)
  • Author(s): 宗崎良太、木下義晶、田尻達郎、代居良太、古賀友紀、住江愛子、孝橋賢一、小田義直、原寿郎、田口智章
  • Organizer: 第20回日本小児泌尿器科学会総会
  • Place of Presentation: 秋田
• [Presentation] 当科で経験した膵外傷症例の検討 (2011)
  • Author(s): 宗崎良太、木下義晶、林田真、松浦俊治、田口智章
  • Organizer: 第48回日本小児外科学会学術集会
  • Place of Presentation: 東京
• [Presentation] 当科で経験した小児膵外傷8例 (2011)
  • Author(s): 宗崎良太、家入里志、木下義晶、林田真、松浦俊治、橋爪誠、田口智章
  • Organizer: 第47回目本腹部救急医学会総会
  • Place of Presentation: 福岡
• [Presentation] The increase of segmental chromosomal abnormalities in proportion to age at diagnosis in neuroblastoma without MYCN mplification (2011)
  • Author(s): Souzaki R, Tajiri T, Kinoshita Y, Teshiba R, Yosue R, Kohashi K, Oda Y, Taguchi T
  • Organizer: ISW2011
  • Place of Presentation: 横浜
• [Presentation] The number of Segmental Chromosome Aberrations Significantly Increases in Proportion To the Age at Diagnosis in Subjects with Diploid/Tetraploid Neuroblastomas without MYCN Amplification (2011)
  • Author(s): Souzaki R, Tajiri T, Teshiba R, Kinoshita Y, Yosue R, Kohashi K, Oda Y, TaguchiT
  • Organizer: 3rd Congress of the International Society of Pediatric Oncology(SIOP2011)
  • Place of Presentation: New Zealand
• [Presentation] 神経芽腫MYCN非増幅症例におけるsegmental chromosome aberrationと年齢との相関 (2011)
  • Author(s): 宗崎良太、出尻達郎、代居良太、木下義晶、二好きな、孝橋賢一、小田義直、田口智章
  • Organizer: 第53回目木小児血液・がん学会学術集会
  • Place of Presentation: 前橋
• [Presentation] 本邦の多施設共同研究における胎児仙尾部奇形腫の周術期合併症および後遺症の検討 (2011)
  • Author(s): 宗崎良太、田口智章、臼井規朗、左勝則、左合治彦、野坂俊介、中村知夫、金森豊、米田光宏、北野良博
  • Organizer: 第9回日本胎児治療学会
  • Place of Presentation: 福岡
• [Presentation] The number of Segmental Chromosome Aberrations Significantly Increases in Proportion To the Age at Diagnosis in Subjects with Diploid/Tetraploid Neuroblastomas without MYCN Amplification (2011)
  • Author(s): Souzaki R, Tajiri T, Teshiba R, Kinoshita Y, Yosue R, Kohashi K, Oda Y, Taguchi T
  • Organizer: SIOP2011
  • Place of Presentation: New Zealand
• [Presentation] The increase of segmental chromosomal abnormalities in proportion to age at diagnosis in neuroblastoma without MYCN mplification (2011)
  • Author(s): Souzaki R, Tajiri T, Teshiba R, Kinoshita Y, Yosue R, Kohashi K, Oda Y, Taguchi T
  • Organizer: ESW2011
  • Place of Presentation: Yokohama
• [Presentation] 神経芽腫におけるHedgenog signal系タンパク発現 (2010)
  • Author(s): 宗崎良太, 他
  • Organizer: 第26回日本小児がん学会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-12-17