Development of a novel treatment for inflammatory bowel disease with Dedifferentiated fat cell(DFAT)
| Project/Area Number |
22791714
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatric surgery
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脱分化脂肪細胞 / 炎症性腸疾患 / 免疫抑制 / DFAT / Busulfan / DSS / IDO / 1-MT(1-methyl-tryptophan) / T細胞増殖抑制 |
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| Research Abstract |
We stimulated peripheral blood T-cells from C57BL/6J mouse with CD3/28 antibody or IL-2. We extracted DFAT from the fat of C57BL/6J mouse, and analyzed the effect of DFAT for the proliferation of the stimulated T-cells. DFAT down-regulated the stimulated T-cells, and showed the high expression levels of the immunosuppressive genes(TRAIL, IDO1, NOS2, HGF, PEG2) stimulated by IFNγ or TNFα. To investigate the effect of T cell suppression in vivo, we intravenously administrated DFAT or PBS to the inflammetory bowel model in mice. Pathologically, DFAT group showed the low grade of the infiltration of T-cells, compared with PBS group.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] Effect of combined treatment with FK506, FTY720, and ex vivo graft irradiation in rat small bowel transplantation : expression of mucosal addressin cell adhesion molecule-12010
Author(s)
Sugito K, Uekusa S, Kawashima H, Masuko T, Furuya T, Konuma N, Ohashi K, Inoue M, Ikeda T, Koshinaga T.
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Journal Title
Pediatric Transplantation
Volume: 14(5)
Pages: 614-617
Related Report
Peer Reviewed
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