Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Research Abstract |
The effects of adipose-derived stem cells(ASCs) seeded on artificial dermis, arteriovenous vascular bundles with free fat, and a vascularized fat flap placed in a tissue engineering chamber(TEC) were investigated. The samples were divided into control and ASCs groups in Experiment 1 with free fat and arteriovenous vascular bundles, and Experiment 2 with vascularized fat. The amount of regenerated tissue was evaluated, and ASCs were traced using 1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethylindocarbocyanine perchlorate(DiI). Results : The regenerated vascularized soft tissue volumes were 2. 12±0. 48 and 2. 20±0. 094 cm^3 in the control and ASCs groups in Experiment 1, respectively, and 1. 30±0. 19 and 1. 36±0. 22 cm^3 in Experiment 2, respectively, showing no significant difference in either experiment(p> 0. 05). No significant difference was also noted on histopathological examination. Moreover, no DiI-labeled cell was observed in the regenerated soft tissue in TEC. These findings suggest that ASCs were necrotized early in TEC.
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