Study ofthe molecular mechanisms of delayed wound healing by using klothomice,which have phenotypessimilar to skin ulcerof aged humans.

Research Project

Project/Area Number	22791726
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Plastic surgery
Research Institution	Sapporo Medical University
Principal Investigator	YAMASHITA Ken 札幌医科大学, 医学部, 助教 (30312507)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	外科 / 老化 / 創傷治癒 / 皮膚潰瘍 / klothoマウス / klotho遺伝子 / 褥創
Research Abstract	The aim of this study was to analyze the molecular mechanisms of delayed wound healing with agingby using α-klotho-deficient (klotho) mice,which have phenotypessimilar to those of aged humans.Wound healing time, histology and mRNA expression were investigated using a skin defect wound model of klothomice. Theklotho mice showed delayed wound healingandimpairedgranulation formation compared to those in wild-type (WT) mice.The reverse transcript polymerase chain reaction (RT-PCR)analysis revealed enhancement of the mRNA expression of interleukin(IL)-1β, IL-6and tumor necrosis factor (TNF)-α, and depressionof laminin-5 in skin wound lesions of klotho mice compared to the levels in WT mice.

Report

(4 results)