Contribution of activated glia cells on the development of sepsis associated encephalopaty

Research Project

Project/Area Number	22791751
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Emergency medicine
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	TAKESHITA Jun 京都府立医科大学, 医学部附属病院, 専攻医 (40433263)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	集中治療医学 / 敗血症
Research Abstract	We created animal model of sepsis suitable for behavioural analysis, including rat intraperitoneally injected lipopolysaccharide(LPS) and mouse treated with Cecal ligation and puncture(CLP). Brain tissue was taken from animals and performed immunohistochemsitry to investigate activation of glial cells during sepsis. In addition, expression level of cytokine mRNA was quantified by real time PCR. In septic animals, glial cells were found to be activated and the amount of pro-inflammatory cytokines increased in the brain.

Report

(3 results)