Osteocytic regulation on osteoclastic bone resorption and survival/death of osteocytes

• Project/Area Number: 22791760
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: SUZUKI Reiko 北海道大学, 大学院・歯学研究科, 専門研究員 (90333723)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞・組織 / 骨細胞 / 破骨細胞 / 細胞・組織学 / 骨 / 破壊細胞 / 歯学

Research Abstract

Osteocytes are shown to synthesize sclerostin and dentin matrix protein-1 (DMP-1), which serve to inhibit osteoblastic activities and subsequent bone remodeling, and regulate surrounding mineralization by binding to calcium ion of calcium phosphate crystals in bone, respectively. In a state of accelerated bone resorption, osteocytes decreased sclerostin synthesis in accordance with stimulated osteoclastic activities. The decreased synthesis of sclerostin appears to be not interrupt accelerated osteoclastic bone resorption by mediating osteoblasts.

Research Products

• [Journal Article] Morphological aspects of the biological function of the osteocytic lacunar canalicular system and of osteocyte-derived factors. (2012)
  • Author(s): Sasaki M., Hongo H., Hasegawa T., Suzuki R., Liu Z., Freitas PHL., Yamada T., Oda K., Yamamoto T., Li M., Totsuka Y., Amizuka N.
  • Journal Title: Oral Science International. Volume: 9(1) Pages: 1-8
  • NAID: 10031144841
  • URL: http://www.sciencedirect.com/science/article/pii/S1348864312000092
  • Peer Reviewed
• [Journal Article] Bone-Orchestrating Cells, Osteocytes. (2012)
  • Author(s): Hongo H., Hasegawa T., Sasaki M., Suzuki R., Yamada T., Shimoji S., Yamamoto T., Amizuka N.
  • Journal Title: Hokkaido Journal of Dental Science. Volume: 32(2) Pages: 93-103
  • NAID: 10030576074
  • URL: http://eprints.lib.hokudai.ac.jp/dspace/handle/2115/48704
  • Peer Reviewed
• [Journal Article] Immunolocalization of sclerostin synthesized by osteocytes in relation to bone remodeling in the interradicular septa of ovariectomized rats. (2012)
  • Author(s): Guo Y., Hasegawa T., Amizuka N., et al
  • Journal Title: Journal of Electron Microscopy Volume: 61(5) Issue: 5 Pages: 309-320
  • DOI: 10.1093/jmicro/dfs052
  • Peer Reviewed
• [Journal Article] The distribution of osteocytic lacunar-canalicular system, and immunolocalization of FGF23 and sclerostin in osteocytes. (2012)
  • Author(s): Amizuka N., Hongo H., Sasaki M., Hasegawa T., Suzuki R., Tabata C., Ubaidus S., Masuki H., Guo Y., Freitas PHL., Oda K., Li M.
  • Journal Title: J Oral Biosci. Volume: 54(1) Pages: 37-42
  • URL: http://www.journaloforalbiosciences.org/article/S1349-0079(12)00008-4/fulltext
  • Peer Reviewed
• [Journal Article] The distribution of osteocytic lacunar-canalicular system, and immunolocalization of FGF23 and sclerostin in osteocytes (2012)
  • Author(s): Amizuka N, Suzuki R et al.
  • Journal Title: J Oral Biosci Volume: 54 Pages: 37-42
  • Peer Reviewed
• [Journal Article] Bone-Orchestrating Cells, Osteocytes (2012)
  • Author(s): Hongo H, Suzuki R et al.
  • Journal Title: Hokkaido Journal of Dental Science Volume: 32 Pages: 93-103
  • NAID: 10030576074
  • Peer Reviewed
• [Journal Article] Morphological aspects on osteocytic function on bone mineralization (2012)
  • Author(s): Sasaki M, Suzuki R et al
  • Journal Title: Oral Science International Volume: 9 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Immunolocalization of sclerostin synthesized by osteocytes in relation to bone remodeling in the interradicular septa of ovariectomized rats (2012)
  • Author(s): Guo Y, Suzuki R et al.
  • Journal Title: J Electron Microsc Volume: 61 Pages: 309-320
  • NAID: 120005300021
  • Peer Reviewed
• [Journal Article] Morphological aspects of the biological function of osteocytic lacunar canalicular system and osteocyte-derived factors (2012)
  • Author(s): Sasaki M, Hongo H, Hasegawa T, Suzuki R, Liu Z, Freitas Paulo HL, Yamada T, Oda K, Yamamoto T , Li M, Totsuka y, Amizuka N
  • Journal Title: Oral Science International Volume: 9 Issue: 1 Pages: 1-8
  • DOI: 10.1016/s1348-8643(12)00009-2
  • NAID: 10031144841
  • Peer Reviewed
• [Journal Article] Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats. (2011)
  • Author(s): Hasegawa T., Li M., Hara K., Sasaki M., Tabata C., Freitas PHL., Hongo H., Suzuki R., Kobayashi M., Inoue K., Yamamoto T., Oohata N., Oda K., Akiyama Y., Amizuka N.
  • Journal Title: Biomed Res. Volume: 32(4) Pages: 259-269
  • NAID: 130004903823
  • URL: https://www.jstage.jst.go.jp/article/biomedres/32/4/32_4_259/_article
  • Peer Reviewed
• [Journal Article] Morphological assessment of bone mineralization in tibial metaphyses of ascorbic arid-deficient ODS rats (2011)
  • Author(s): Hasegawa, Suzuki, et al.
  • Journal Title: Biomed.Res. Volume: 32 Pages: 259-269
  • Peer Reviewed
• [Journal Article] Ultrastructural observation on cells meeting the histological criteria for preosteoblasts a study in the mouse tibial methaphysis (2010)
  • Author(s): Narimatsu K, Li M, Freitas Paulo HL, Sultana S, Ubaidus S, Kojima T, Liu Z, Guo Y, Suzuki R, Yamamoto T , Oda k, Amizuka N
  • Journal Title: J Electron Microsc Volume: 59 Issue: 5 Pages: 427-436
  • DOI: 10.1093/jmicro/dfq021
  • Peer Reviewed
• [Journal Article] Ultrastructural Assessment of Mineral Crystallization and Collagen Mineralization in Bone. (2010)
  • Author(s): Li M., Hasegawa T., Masuki H., Liu Z., Guo Y., Suzuki R., Yamamoto T., Freitas PHL., Amizuka N.
  • Journal Title: J Oral Biosci. Volume: 52(2) Pages: 94-99
  • NAID: 10027105502
  • URL: https://www.jstage.jst.go.jp/article/joralbiosci/52/2/52_2_94/_article
  • Peer Reviewed
• [Journal Article] Histological review of the human cellular cementum with special reference to an alternating lamellar pattern (2010)
  • Author(s): Yamamoto T., Li M., Liu Z., Guo Y., Hasegawa T., Masuki H., Suzuki R., Amizuka N
  • Journal Title: Odontol Volume: 98 Issue: 2 Pages: 102-109
  • DOI: 10.1007/s10266-010-0134-3
  • NAID: 10029302597
  • Peer Reviewed
• [Journal Article] Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoproteger in deficient mice. (2010)
  • Author(s): Masuki H., Li M., Hasegawa T., Suzuki R., Guo Y., Liu Z., Oda K., Yamamoto T., Kawanami M., Amizuka N.
  • Journal Title: Biomed Res. Volume: 31(5) Pages: 307-318
  • URL: https://www.jstage.jst.go.jp/article/biomedres/31/5/31_5_307/_article
  • Peer Reviewed
• [Journal Article] Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice. (2010)
  • Author(s): Masuki H., Suzuki R., et al.
  • Journal Title: Biomed Res. Volume: 31 Pages: 307-318
  • NAID: 130004903810
  • Peer Reviewed
• [Journal Article] Ultrastructural observation on cells meeting the histological criteria for preosteoblasts--astudy in the mouse tibial metaphysis. (2010)
  • Author(s): Narimatsu K., Suzuki R., et al.
  • Journal Title: J Electron Microsc (Tokyo) Volume: 59 Pages: 427-436
  • Peer Reviewed
• [Journal Article] Histological review of the human cellular cementum with special reference to an alternating lamellar pattern. (2010)
  • Author(s): Yamamoto T., Suzuki R., et al.
  • Journal Title: Odontology Volume: 98 Pages: 102-109
  • NAID: 10029302597
  • Peer Reviewed
• [Presentation] 副甲状腺ホルモン投与による骨細胞性骨溶解に関する組織化学的検索. (2012)
  • Author(s): 本郷裕美、佐々木宗輝、長谷川智香、鈴木礼子、下地伸司、網塚憲生
  • Organizer: 第117回日本解剖学会総会・全国学術集会ベルクラシック甲府
  • Place of Presentation: 山梨県
• [Presentation] 副甲状腺ホルモン投与による骨細胞性骨溶解の可能性について. (2011)
  • Author(s): 本郷裕美、佐々木宗輝、長谷川智香、郭頴、虎谷彌、田幡千尋、鈴木礼子、李敏啓、網塚憲生
  • Organizer: 第53回歯科基礎医学会学術大会長良川国際会議場
  • Place of Presentation: 岐阜県
• [Presentation] 副甲状腺ホルモン投与による骨細胞性骨溶解の可能性について (2011)
  • Author(s): 本郷裕美、鈴木礼子, ほか
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場(岐阜市)
• [Presentation] Histochemical assessment for altered bone tissue in transgenic mice over-expressing parathyroid hormone-related peptide (PTHrP) driven by type I collagen promoter (2010)
  • Author(s): Li M.Suzuki R., et al.
  • Organizer: The 32^<nd> Annual Meeting of American Society for Bone and Mineral Research
  • Place of Presentation: Canada, Toronto
  • Year and Date: 2010-10-17
• [Presentation] オステオプロテジェリン遺伝子欠損マウスにおける骨細胞産生蛋白の局在. (2010)
  • Author(s): 増木英郎、李敏啓、郭頴、長谷川智香、柳鋳晟、鈴木礼子、織田公光、山本恒之、川浪雅光、網塚憲生
  • Organizer: 第52回歯科基礎医学会学術大会タワーホール船橋
  • Place of Presentation: 東京都
• [Presentation] アスコルビン酸欠乏ラットの歯根膜異常における組織化学的検索. (2010)
  • Author(s): 長谷川智香、李敏啓、井上貴一朗、田幡千尋、佐々木宗輝、鈴木礼子、織田公光、山本恒之、大畑昇、網塚憲生
  • Organizer: 第52回歯科基礎医学会学術大会タワーホール船橋
  • Place of Presentation: 東京都
• [Presentation] 有細胞セメント質層板構造に関する走査型および透過型電子顕微鏡検索. (2010)
  • Author(s): 山本恒之、李敏啓、郭頴、柳鋳晟、増木英郎、長谷川智香、鈴木礼子、網塚憲生
  • Organizer: 第52回歯科基礎医学会学術大会タワーホール船橋
  • Place of Presentation: 東京都
• [Presentation] RANKL遺伝子欠損マウスの骨芽細胞系細胞における組織学的解析. (2010)
  • Author(s): 宮本幸奈、李敏啓、長谷川智香、柳鋳晟、郭頴、鈴木礼子、沢村祥子、宇田川信之、山本恒之、網塚憲生
  • Organizer: 第30回日本骨形態計測学会米子コンベンションセンター
  • Place of Presentation: 鳥取県
• [Presentation] typeIcollagenpromoterを用いたPTHrPTgマウスの骨芽細胞における解析. (2010)
  • Author(s): 李敏啓、長谷川智香、柳鋳晟、郭穎、鈴木礼子、沢村祥子、山本恒之、網塚憲生
  • Organizer: 第30回日本骨形態計測学会米子コンベンションセンター
  • Place of Presentation: 鳥取県
• [Remarks]
  • URL: http://www.den.hokudai.ac.jp/anatomy2/hokudai_d/index.html