| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
P. gingivalis are invaginated by gingival epithelial cells via the endocytic pathway, and some intracellular bacteria are sorted to lytic compartments, including autolysosomes and late endosomes/lysosomes, while a considerable number of the remaining organisms are sorted to recycling endosomes, followed by bacterial exit from the cells. Exited bacteria can re-enter fresh cells. This result strongly suggests that the recycling pathway is exploited by gingivalis to exit from infected cells to neighbouring cells as intracellular P. a mechanism of cell-to-cell spreading.Knockdown of the combinational SNARE proteins Vti1b and VAMP8 with siRNAs disturbs the fusion of GcAV (GAS containing Autophagosome like Vacuole) with lysosome. This finding indicates that VAMP8 and Vti1b mediate fusion with lysosomes in antimicrobial autophagy.
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