Tooth morphogenesis and the regulation of phosphate homeostasis : basic and preclinical studies of tooth hypoplasia

• Project/Area Number: 22791768
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: YOSHIOKA Hirotaka 広島大学, 大学院・医歯薬学総合研究科, 助教 (50523411)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リン酸代謝 / 歯牙形成 / 石灰化 / エナメル質 / 歯

Research Abstract

Microarray analysis identified the odontogenesis-related genes influenced by inorganic phosphate(Pi) overload. Among the genes identified by microarray analysis, fibromodulin was downregulated in an extracellular Pi level-dependent manner in both cultured tooth germs and differentiated ameloblast lineage cells. This suppression of fibromodulin gene was dependent on ERK1/ 2-Egr1 signaling pathway in differentiated ameloblast lineage cells. These data suggest that the fibromodulin may be involved in the etiology of enamel hypoplasia caused by hyperphosphatemia.

Research Products

• [Journal Article] The EP4-ERK-dependent pathway stimulatesosteo-adipogenic progenitor proliferation resulting in increased adipogenesis in fetal rat calvariacell cultures (2012)
  • Author(s): Minamizaki T, Yoshiko Y, Yoshioka H, Kozai K, Aubin JE, Maeda N
  • Journal Title: Prostaglandins and Other Lipid Mediators Volume: 97 Pages: 97-102
  • Peer Reviewed
• [Journal Article] Incisor enamel formation is impairedin transgenic rats overexpressing the type III NaPi transporter Slc20a1 (2011)
  • Author(s): Yoshioka H, Yoshiko Y, Minamizaki T, Suzuki S, Koma Y, Nobukiyo A, SotomaruY, Suzuki A, Itoh M and Maeda N
  • Journal Title: Calcified Tissue International Volume: 89 Pages: 192-202
  • Peer Reviewed
• [Journal Article] Incisor Enamel Formation is Impaired in Transgenic Rats Overexpressing the Type III NaPi Transporter Slc20al (2011)
  • Author(s): Hirotaka Yoshioka, Yuji Yoshiko, Tomoko Minamizaki, Sayaka Suzuki, Yoshiro Koma, Asako Nobukiyo, Yusuke Sotomaru, Atsushi Suzuki, Mitsuyasu Itoh, Norihiko Maeda
  • Journal Title: Calcified Tissue International Volume: 89 Issue: 3 Pages: 192-202
  • DOI: 10.1007/s00223-011-9506-0
  • Peer Reviewed
• [Journal Article] 1α, 25(OH) 2D acts predominantly onmature osteoblastic cells under conditions of high extracellular phosphate to increase FGF23 productionin vitro (2010)
  • Author(s): Yamamoto R, Minamizaki T, Yoshiko Y, Yoshioka H, Tanne S, Aubin JE, Maeda N
  • Journal Title: Journal of Endocrinology Volume: 206 Pages: 279-286
  • Peer Reviewed
• [Presentation] Phosphate Regulates Fibromodulin Expression Tlarough the ERK Pathway in Ameloblasts (2012)
  • Author(s): 吉岡広陽
  • Organizer: 41^<st> Annual Meeting of the American Association for Dental Research
  • Place of Presentation: アメリカ合衆国フロリダ州タンパ
  • Year and Date: 2012-03-23
• [Presentation] Phosphate Regulates Fibromodulin Expression Through theERK Pathway in Ameloblasts (2012)
  • Author(s): Yoshioka H, Yoshiko Y, Irie Y, Minamizaki T, Kato Y, Sugiyama T, Maeda N
  • Organizer: The 41^<st> Annual Meeting of the AmericanAssociation for Dental Research
  • Place of Presentation: Tampa, FL, USA
• [Presentation] 細胞外リン酸はERK1/2シグナルを介してエナメル芽細胞におけるFibromodulin遺伝子の発現を抑制する (2011)
  • Author(s): 入江泰正
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 岐阜県岐阜市
  • Year and Date: 2011-10-01
• [Presentation] 細胞外リン酸はERK1/ 2シグナルを介してエナメル芽細胞におけるFibromodulin遺伝子の発現を抑制する (2011)
  • Author(s): 入江泰正, 吉岡広陽, 吉子裕二, 南崎朋子, 加藤幸夫, 前田憲彦
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] Shimizu A, AubinJ, Maeda N. MEPE-Derived Phosphorylated ASARM Is Involved inFGF23/ soluble Klotho-DependentHypomineralization (2011)
  • Author(s): Konishi Y, Yoshiko Y, Minamizaki T, Yoshioka H, Maeda M, Shimizu A, AubinJ, Maeda N
  • Organizer: The 33rd AnnualMeeting of the American Society forBone and Mineral Research
  • Place of Presentation: San Diego, CA, USA
• [Presentation] FGFシグナルによる石灰化調節:FGF23-Phex経路を中心として (2011)
  • Author(s): 南崎朋子, 吉子裕二, 小西有希子, 吉岡広陽, 香西克之, 前田憲彦
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] MEPE由来リン酸化ASARMとPhexはFGF23/可溶型Klothoによる石灰化の負の調節系と関連する (2011)
  • Author(s): 小西有希子, 吉子裕二, 南崎朋子, 吉岡広陽, 香西克之, 前田憲彦
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] Overexpression of the type III sodium-dependent phosphate transporter Pit1 markedly perturbs enamel formation during tooth development (2010)
  • Author(s): 吉岡広陽
  • Organizer: 2010 Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: カナダ オンタリオ州トロント
  • Year and Date: 2010-10-17
• [Presentation] III型ナトリウム依存性リン酸トランスポーターPit1の過剰発現は歯のエナメル質形成に影響し,骨・ミネラル代謝異常の発症とともに増悪する (2010)
  • Author(s): 錦織亜矢
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: 東京都江戸川区
  • Year and Date: 2010-09-22
• [Presentation] III型ナトリウム依存性リン酸トランスポーターPit1の過剰発現は骨・ミネラル代謝に先行して歯のエナメル質形成に障害をきたす (2010)
  • Author(s): 吉岡広陽
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2010-07-21
• [Presentation] Overexpression of the type IIIsodium-dependent phosphatetransporter Pit1 markedly perturbsenamel formation during toothdevelopment (2010)
  • Author(s): Yoshioka H, Yoshiko Y, Minamizaki T, Suzuki A, Itoh M, Maeda N
  • Organizer: The32nd Annual Meetingof the American Society for Bone andMineral Research
  • Place of Presentation: Toronto, Canada
• [Presentation] Soluble Klotho Acts as a Coactivator of FGF23 in Bone but notin Kidney to Regulate Mineralization (2010)
  • Author(s): Minamizaki T, Yoshiko Y, Konishi Y, Yoshioka H, Kozai K, Aubin JE, MaedaN
  • Organizer: The32nd Annual Meeting of theAmerican Society for Bone and MineralResearch
  • Place of Presentation: Toronto, Canada
• [Presentation] III型ナトリウム依存性リン酸トランスポーターPit1の過剰発現は歯のエナメル質形成に影響し,骨・ミネラル代謝異常の発症とともに増悪する (2010)
  • Author(s): 錦織亜矢, 吉岡広陽, 吉子裕二, 南崎朋子, 前田憲彦
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
• [Presentation] 可溶型Klothoは骨におけるFGF23の作用発現にリクルートされる (2010)
  • Author(s): 南崎朋子, 吉子裕二, 吉岡広陽, 前田憲彦
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] III型ナトリウム依存性リン酸トランスポーターPit1の過剰発現は骨・ミネラル代謝に先行して歯のエナメル質形成に障害をきたす (2010)
  • Author(s): 吉岡広陽, 吉子裕二, 南崎朋子, 鈴木敦詞, 伊藤光泰, 前田憲彦
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] 老化関連タンパクKlothoによる骨の石灰化調節機構 (2010)
  • Author(s): 小西有希子, 吉子裕二, 南崎朋子, 北畠聖子, 吉岡広陽, 香西克之, 前田憲彦
  • Organizer: 第43回広島大学歯学会総会
  • Place of Presentation: 広島
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/anato1/welcome.htm