Clarification of the disintegration of Hertwig's epithelial root sheath by using a real time imaging

• Project/Area Number: 22791774
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: OTSU Keishi 岩手医科大学, 歯学部, ポストドクター (60509066)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 歯根 / 発生 / Hertwig's上皮鞘 / EMT / ROCK / Hertwig上皮鞘

Research Abstract

Tooth root formation is initiated by the development of Hertwig's epithelial root sheath(HERS) from the cervical loop in the enamel organ. However, the cellular dynamics during HERS formation is not well known yet. In This study, we aimed to establish the experimental system to visualize the dynamics of HERS cells in order to elucidate the mechanism of HERS disintegration by EMT. We established the real time slice culture imaging system, which could observe the migration, division, and actin promoter activity in HERS cell. We also established HERS cell line from mouse molars. The cells expressed mesenchymal cell markers, and the expressions were increased to the stimulation of TGF-β. The root elongation was intensified by HGF with the increased cell proliferation, indicating the implication between HGF signaling and tooth root formation.

Research Products

• [Journal Article] Histological analysis of epithelial stem cells during induced pluripotent stem cell-derived teratoma development (2012)
  • Author(s): Kishigami, R., Otsu, K., Oikawa-Sasaki, A., Fujiwara, N., Ishizeki, K., Tabata, Y., Harada, H.
  • Journal Title: J. Oral Biosci Volume: 54 Pages: 54-58
• [Journal Article] Differentiation of induced pluripotent stem cells into dental mesenchymal cells (2012)
  • Author(s): Otsu, K., Kishigami, R., Oikawa-Sasaki, A., Fukumoto, S., Yamada, A., Fujiwara, N., Ishizeki, K. Harada, H.
  • Journal Title: Stem Cells & Dev Volume: (in press)
• [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation (2012)
  • Author(s): Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 10590-10601
• [Journal Article] エナメル質の横紋形成メカニズムの解明 (2012)
  • Author(s): 及川愛, 大津圭史, 藤原尚樹, 石関清人, 中富満城, 大島勇人, 原田英光
  • Journal Title: 岩医大歯誌 Volume: (in press)
  • NAID: 110009445045
• [Journal Article] Histological analysis of epithelial stem cells during induced pluripotent stem cell-derived teratoma development (2012)
  • Author(s): Kishigami, R., Otsu, K., Oikawa-Sasaki, A., Fujiwara, N., Ishizeki, K., Tabata, Y., Harada, H.
  • Journal Title: J.Oral Biosci. Volume: 54 Pages: 54-58
  • Peer Reviewed
• [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation (2012)
  • Author(s): Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
  • Journal Title: J Biol Chem Volume: 287(13) Issue: 13 Pages: 10590-601
  • DOI: 10.1074/jbc.m111.285874
  • Peer Reviewed
• [Journal Article] Establishment of Hertwig's epithelial root sheath cell line from cells involved in epithelial-mesenchymal transition (2011)
  • Author(s): Akimoto, T., Fujiwara, N., Kagiya, T., Otsu, K., Ishizeki, K., Harada, H.
  • Journal Title: BBRC Volume: 404 Pages: 308-312
• [Journal Article] Functional role of Rho-kinase in ameloblast differentiation (2011)
  • Author(s): Otsu, K., Kishigami, R., Fujiwara, N., Ishizeki, K., Harada, H
  • Journal Title: J. Cell Physiol Volume: 226 Pages: 2527-2534
• [Journal Article] Hepatocyte growth factor stimulates root growth during the development of mouse molar teeth (2011)
  • Author(s): Sakuraba, H., Fujiwara, N., Sasaki-Oikawa, A., Sakano, M., Otsu, K., Ishizeki, K., Harada, H.
  • Journal Title: J. Period. Res Volume: 47 Pages: 81-88
• [Journal Article] 再生医学に関する新設講義の受講アンケートの結果と考察 (2011)
  • Author(s): 原田英光, 大津圭史, 藤原尚樹, 石関清人, 及川愛
  • Journal Title: 日歯教誌 Volume: 27 Pages: 63-68
  • NAID: 10029548262
• [Journal Article] Establishment of Hertwig's epithelial root sheath cell line from cells invo lved in epithelial-mesenchymal transition (2011)
  • Author(s): Akimoto, T., Fujiwara, N., Kagiya, T., Otsu, K., Ishizeki, K., Harada, H.
  • Journal Title: Biochem Biophys Res Commun Volume: 404 Pages: 308-312
  • Peer Reviewed
• [Journal Article] Functional role of Rho-kinase in ameloblast differentiation (2011)
  • Author(s): Otsu, K., et al
  • Journal Title: J.Cell Physiol. Volume: Vol.226 Issue: 10 Pages: 2527-2534
  • DOI: 10.1002/jcp.22597
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor stimulates root growth during the development of mouse molar teeth (2011)
  • Author(s): Sakuraba, H., Fujiwara, N., Sasaki-Oikawa, A., Sakano, M., Otsu, K., Ishizeki, K., Harada, H.
  • Journal Title: J.Period.Res. Volume: 47 Issue: 1 Pages: 81-88
  • DOI: 10.1111/j.1600-0765.2011.01407.x
  • Peer Reviewed
• [Journal Article] Differentiation of induced pluripotent stem cells into dental mesenchymal cells (2011)
  • Author(s): Otsu, K., et al
  • Journal Title: Stem Cells Dev. Volume: (e-Pub) Issue: 7 Pages: 1156-1164
  • DOI: 10.1089/scd.2011.0210
  • Peer Reviewed
• [Journal Article] Establishment of Hertwig's epithelial root sheath cell line from cells involved in epithelial-mesenchymal transition (2010)
  • Author(s): Tadashi Akimoto
  • Journal Title: Biochem Biophys Res Commun Volume: 40 Pages: 308-312
  • Peer Reviewed
• [Journal Article] Functional Role of Rho-kinase in Ameloblast Differentiation (2010)
  • Author(s): Keishi Otsu
  • Journal Title: J.Cell Physiol. Volume: (In press)
  • Peer Reviewed
• [Presentation] ライブイメージングでとらえる歯の発生メカニズム (2012)
  • Author(s): 大津圭史, 原田英光
  • Organizer: 第117回日本解剖学会総会・全国学術大会研究集会・懇話会
  • Place of Presentation: 山梨大学(山梨)
  • Year and Date: 2012-03-26
• [Presentation] Hidemitsu Harada The role of Rho signaling pathway in dental epithelial stem cells (2012)
  • Author(s): Keishi Otsu, Ryota Kishigami, Ai Oikawa-Sasaki, Naoki Fujiwara, Kiyoto Ishizeki
  • Organizer: 第117回日本解剖学会総会・全国学術大会
  • Place of Presentation: 山梨大学(山梨)
  • Year and Date: 2012-03-26
• [Presentation] ライブイメージングでとらえる歯の発生メカニズム (2012)
  • Author(s): 大津圭史, 原田英光
  • Organizer: 第117回日本解剖学会総会・全国学術大会研究集会・懇話会
  • Place of Presentation: 山梨大学(山梨)(招待講演)
  • Year and Date: 2012-03-26
• [Presentation] The role of Rho signaling pathway in dental epithelial stem cells (2012)
  • Author(s): Keishi Otsu, Ryota Kishigami, Ai Oikawa-Sasaki, Naoki Fujiwara, Kiyoto Ishizeki, Hidemitsu Harada
  • Organizer: 第117回日本解剖学会総会・全国学術大会研究集会・懇話会
  • Place of Presentation: 山梨大学(山梨)
  • Year and Date: 2012-03-26
• [Presentation] The role of Rho signaling pathway in dental epithelial stem cells (2012)
  • Author(s): Keishi Otsu, Hidemitsu Harada
  • Organizer: Gordon Research Conference, Craniofacial development an regenerative medicine
  • Place of Presentation: CA, U. S. A
• [Presentation] The role of Rho signaling pathway in dental epithelial stem cells (2012)
  • Author(s): Keishi Otsu, Hidemitsu Harada
  • Organizer: Gordon Research Conference, Craniofacial development an regenerative medicine
  • Place of Presentation: CA, U.S.A.
• [Presentation] 歯の再生に向けたiPS細胞から歯原性間葉細胞への分化誘導 (2011)
  • Author(s): 大津圭史
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場・岐阜市
  • Year and Date: 2011-10-02
• [Presentation] Hepatocyte growth factorはマウス臼歯歯胚の歯根形成を促進する (2011)
  • Author(s): 藤原尚樹, 櫻庭春菜, 坂野深香, 佐々木, 及川愛, 大津圭史, 石関清人, 原田英光
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2011-10-01
• [Presentation] ツチ骨とキヌタ骨の形成とメッケル軟骨との関連性 (2011)
  • Author(s): 石関清人, 大津圭史, 藤原尚樹, 原田英光
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Year and Date: 2011-09-30
• [Presentation] アメロゲニンの概日的発現周期に関わるMsx2の役割 (2011)
  • Author(s): 及川愛, 大津圭史, 藤原尚樹, 石関清人, 中冨満城, 大島勇人, 原田英光
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2011-09-30
• [Presentation] Neural Crest like Cell from Induced Pluripotent Stem Cells for Tooth Regeneration (2011)
  • Author(s): 大津圭史
  • Organizer: Frontier Meeting
  • Place of Presentation: 日大会館(東京都)
• [Presentation] Establishment of Hertwig's epithelial root sheath cell line from cells involved in epithelial-mesenchymal transition (2011)
  • Author(s): 藤原尚樹、大津圭史
  • Organizer: Frontier Meeting
  • Place of Presentation: 日大会館(東京都)
• [Presentation] Functional role of Rho signaling in dental epithelial stem cells (2011)
  • Author(s): Otsu, K
  • Organizer: Gordon Research Conference, Bone and Tooth
  • Place of Presentation: Les Diablerets, Switzerland
• [Presentation] ツチ骨とキヌク骨の形成とメッケル軟骨との関連性 (2011)
  • Author(s): 石関清人, 大津圭史, 藤原尚樹, 原田英光
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場(岐阜)
• [Presentation] iPS細胞由来奇形腫における上皮細胞の分化解析と歯の再生への可能性 (2011)
  • Author(s): 岸上良太
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
• [Presentation] Live cell imaging of dental epithelial stem cells (2011)
  • Author(s): 原田英光
  • Organizer: 第116回日本解剖学会総会全国学術集会
  • Place of Presentation: 横浜
• [Presentation] Hertwig上皮鞘細胞の特性とEMTの可能性 (2011)
  • Author(s): 藤原尚樹
  • Organizer: 第115回日本解剖学会総会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] 歯の再生に向けたiPS細胞から歯原性間葉細胞への分化誘導 (2011)
  • Author(s): 大津圭史
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] PS細胞由来奇形腫における上皮細胞の分化解析と歯の再生への応用 (2010)
  • Author(s): 岸上良太、大津圭史
  • Organizer: 岩手医科大学歯学会第70回例会
  • Place of Presentation: 岩手医大(盛岡)
  • Year and Date: 2010-07-03
• [Presentation] iPS細胞由来奇形腫における上皮細胞の分化解析と歯の再生への応用 (2010)
  • Author(s): 岸上良太
  • Organizer: 岩手医科大学歯学会第70回例会
  • Place of Presentation: 岩手医大(盛岡)
  • Year and Date: 2010-07-03
• [Presentation] エナメル横紋形成のメカニズムに関する新規仮説 (2010)
  • Author(s): 佐々木愛、大津圭史
  • Organizer: 岩手医科大学歯学会第69回例会
  • Place of Presentation: 岩手医大(盛岡)
  • Year and Date: 2010-02-27
• [Presentation] Role of Rho kinase in amelogenesis (2010)
  • Author(s): Keishi Otsu
  • Organizer: Gordon Research Conferences
  • Place of Presentation: ll Ciocco Hotel and Resort, Lucca, Italy
• [Presentation] Live cell imaging of Hertwig's epithelial root sheath formation process (2010)
  • Author(s): Hidemitsu Harada, Keishi Otsu
  • Organizer: Gordon Research Conferences
  • Place of Presentation: ll Ciocco Hotel and Resort, Lucca, Italy
• [Presentation] New hypothesis of cross-striation formation mechanism (2010)
  • Author(s): Ai Sasaki, Keishi Otsu
  • Organizer: 10^<th> International Conference on Tooth Morphogenesis and Differentiation
  • Place of Presentation: Berlin, Germany
• [Presentation] Neural crest like cells from induced pluripotent stem cells for tooth regeneration (2010)
  • Author(s): Ryota Kishigami, Keishi Otsu
  • Organizer: International Conference on Tooth Morphogenesis and Differentiation
  • Place of Presentation: Berlin, Germany
• [Presentation] Visualizing cellular dynamics of tooth development (2010)
  • Author(s): Keishi Otsu
  • Organizer: International Conference on Tooth Morphogenesis and Differentiation
  • Place of Presentation: Berlin, Germany
• [Presentation] Cell culture of human dental epithelial cells and its approach for regenerative dentistry. (2010)
  • Author(s): Hisayo Mayama, Keishi Otsu
  • Organizer: International Conference on Tooth Morphogenesis and Differentiation
  • Place of Presentation: Berlin, Germany
• [Presentation] Establishment of immortalized Hertwig's epithelial root sheath cells and character of cell line (2010)
  • Author(s): Naoki Fujiwara, Keishi Otsu
  • Organizer: International Conference on Tooth Morphogenesis and Differentiation
  • Place of Presentation: Berlin, Germany
• [Presentation] Role of Rho kinase in amelogenesis (2010)
  • Author(s): Keishi Otsu
  • Organizer: Gordon Research Conferences
  • Place of Presentation: 11 Ciocco Hotel and Resort, Lucca, Italy
• [Presentation] Llive cell imaging of Hertwig's epithelial root sheath formation process (2010)
  • Author(s): Hidemitsu Harada
  • Organizer: 11 Gordon Research Conferences
  • Place of Presentation: Ciocco Hotel and Resort, Lucca, Italy
• [Presentation] Cell culture of human dental epithelial cells and its approach for regenerative dentistry (2010)
  • Author(s): Hisayo Mayama
  • Organizer: International Conference on Tooth Morphogenesis and Differentiation
  • Place of Presentation: Berlin, Germany
• [Presentation] Hertwig上皮鞘細胞の特性とEMTの可能性
  • Author(s): 藤原尚樹, 大津圭史
  • Organizer: 第115回日本解剖学会総会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] iPS細胞由来奇形腫における上皮細胞の分化解析と歯の再生への可能性
  • Author(s): 岸上良太、大津圭史
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
• [Presentation] 歯の再生に向けたiPS細胞から神経堤細胞への分化誘導
  • Author(s): 大津圭史
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
• [Presentation] Live cell imaging of dental epithelial stem cells
  • Author(s): 原田英光、大津圭史
  • Organizer: 第116回日本解剖学会総会全国学術集会
  • Place of Presentation: 横浜
• [Book] Organ Cultures and Kidney-Capsule Grafting of tooth germs(Eds ; Chrissa Kioussi Odontogenesis)(Methods and Protocols) (2012)
  • Author(s): Keishi Otsu, Naoki Fujiwara, Hidemitsu Harada
  • Publisher: Human Press SPRINGEE SCIENCE(In press)
• [Book] Organ Cultures and Kidney-Capsule Grafting of tooth germs
  • Author(s): Keishi Otsu, Naoki Fujiwara, Hidemitsu Harada
  • Publisher: Human Press SPRINGER SCIENCE(in press)
• [Remarks]
  • URL: http://oralhist.iwate-med.ac.jp/
• [Remarks]
  • URL: http://oralhist.iwate-med.ac.jp/