The new strategy of the replacement therapy for prevention of dental caries

• Project/Area Number: 22791775
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Showa University
• Principal Investigator: TAKAHASHI Masakazu 昭和大学, 歯学部, 兼任講師 (40420921)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 口腔細菌学 / 多糖分解酵素発現株 / リプレースメントセラピー / 齲蝕予防 / リプレイスメントセラピー / ムタナーゼ / 多糖分解酵素 / 齲蝕原性バイオフィルム / 多糖分解酵素産生菌

Research Abstract

In order to construction of polysaccharide degradation enzymes producing bacteria for caries prevention by replacement therapy, we firstly identified and analyzed the genes encoding a mutanase (water insoluble glucan degradation enzyme). Secondarily, we constructed the bacterium which is overexpress of polysaccharide degradation enzymes by using plasmid-borne expression system.

Research Products

• [Journal Article] Rapid detection and identification of Streptococcus ratti by a species-specific PCR method (2011)
  • Author(s): Nishio J, Taniguchi M, Higashi J, Takahashi M, Ando T, Hasegawa T, Igarashi T
  • Journal Title: Anaerobe Volume: 18 Issue: 1 Pages: 44-47
  • DOI: 10.1016/j.anaerobe.2011.09.001
  • Peer Reviewed
• [Journal Article] Rapid detection and identification of Streptococcus ratti by a species-specific PCR method (2011)
  • Author(s): Nishio J, Taniguchi M, Higashi J, Takahashi M, Ando T, Hasegawa T, Igarashi T
  • Journal Title: Anaerobe Volume: 18 Pages: 44-47
  • Peer Reviewed
• [Journal Article] Molecular Analysis of the Nuclease Anchored to the Cell Wall of Streptococcus gordonii (2010)
  • Author(s): Takahashi M, Igarashi T
  • Journal Title: Dental Medicine Research Volume: 30(3) Pages: 219-227
  • NAID: 10027973274
  • Peer Reviewed
• [Presentation] Branched-chain Amino Acids Utilization by Streptococcus mutans (2010)
  • Author(s): Arimoto T, Taniguchi M, Takahashi M, Igarashi T
  • Organizer: 第58回国際歯科研究学会日本部会
  • Place of Presentation: 北九州
  • Year and Date: 2010-11-21
• [Presentation] Branched-chain Amino Acids Utilization by Streptococcus mutans (2010)
  • Author(s): T.Arimoto, M.Taniguchi, M.Takahashi, T.Igarashi
  • Organizer: 第58回国際歯科研究学会日本部会
  • Place of Presentation: 九州歯科大学
  • Year and Date: 2010-11-21
• [Presentation] TLR2-dependent inflammatory response by Actinomyces viscosus is attenuated by naringenin (2010)
  • Author(s): Shimada E, Kataoka H, Takahashi M,Yamamoto M, Igarashi T
  • Organizer: 第96回アメリカ歯周病学会共催日本歯周病学会2010年大会
  • Place of Presentation: ハワイ
  • Year and Date: 2010-10-30
• [Presentation] TLR2-dependent inflammatory response by Actinomyces visucosus is attenuated by naringenin (2010)
  • Author(s): Eri Shimada, Hideo Kataoka, Masakazu Takahashi, Takeshi Igarashi, Matsuo Yamamoto
  • Organizer: 第96回アメリカ歯周病学会共催日本歯周病学会2010年大会
  • Place of Presentation: ハワイコンベンションセンター
  • Year and Date: 2010-10-30
• [Presentation] Prevotella intermediaのフルクタン代謝関連遺伝子群の解析 (2010)
  • Author(s): 布施晴香, 深町はるか, 高橋真和, 井上美津子, 五十嵐武
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2010-09-21
• [Presentation] Prevotella intermedia のフルクタン代謝関連遺伝子群の解析 (2010)
  • Author(s): 布施晴香, 深町はるか,高橋真和,井上美津子,五十嵐武
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-20
• [Presentation] Actinomyces viscosusのリポタンパク質はTLR2を介した炎症応答を誘導する (2010)
  • Author(s): 島田絵里,片岡嗣雄,高橋真和,山本松男,五十嵐武
  • Organizer: 第30回昭和歯学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-03
• [Presentation] Naringenin Suppresses the Inflammatory Response Induced by Staphylococcus aureus (2010)
  • Author(s): Shimada E, Kataoka H, Takahashi M,Yamamoto M, Igarashi T
  • Organizer: 110thAmerican society for microbiology general meeting
  • Place of Presentation: サンディエゴ
  • Year and Date: 2010-03-23