Identification and localization of new human pulpal stem cell markers for regenerative medicine studies

• Project/Area Number: 22791780
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: KOBAYASHI Tomoko 日本歯科大学, 生命歯学部, 助教 (10548283)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
• Keywords: マイクロアレイ / 細胞・組織 / 再生医学 / 歯学 / 成体幹細胞 / 分化 / 遺伝子 / シグナル伝達

Research Abstract

Fifty clones derived from human dental pulp cells in primary culture were incubated for a long period until they senesced. Gene expression profiles were analyzed between representative 2 clones which had different differentiation capacities each other. One clone exhibiting osteogenic, chondrogenic and adipogenic differentiation abilities displayed high expression of cell cycle- and cell division-relating genes compared with another clone with osteogenic, chondrogenic but not adipogenic differentiation abilities. New candidate genes for identifying human dental pulp stem cells may be included within the range of cell cycle- and cell division-relating genes and their transcriptional factors.

Research Products

• [Presentation] Isolation of clonal dental pulp cells with different differentiation potentials (2013)
  • Author(s): Kobayashi T, Torii D, Tsutsui TW, Tsutsui T
  • Organizer: The 91st General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: Washington State Convention Center, Seattle, U.S.A..
  • Year and Date: 2013-03-22
• [Presentation] 培養ヒト歯髄細胞の多分化能の維持と細胞増殖-細胞クローン解析- (2011)
  • Author(s): 筒井健機, 小林朋子, 鳥居大祐, 肖黎, 筒井健夫
  • Organizer: 日本口腔組織培養学会
  • Place of Presentation: 明海大学浦安キャンパス(千葉県)
  • Year and Date: 2011-11-19
• [Presentation] 培養ヒト歯髄細胞クローン解析:多分化能と増殖能 (2011)
  • Author(s): 小林朋子、鳥居大祐、筒井健夫,肖黎、筒井健機
  • Organizer: 第53回歯科基礎医学会学術開会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-02
• [Presentation] 培養ヒト歯髄細胞クローン解析:多分化能と増殖能 (2011)
  • Author(s): 小林朋子, 鳥居大祐, 筒井健夫, 肖黎, 筒井健機
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-02
• [Presentation] Isolation of clonal dental pulp cells with different differentiation potentials
  • Author(s): Kobayashi T, Torii D, Tsutsui TW and Tsutsui T
  • Organizer: The 91st General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: Washington State Convention Center, Seattle, U. S. A.