Validation of the expression and function of Abca5 in osteoclasts and bone tissue.

• Project/Area Number: 22791786
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Chubu University
• Principal Investigator: NAKAGAWA Hiroshi 中部大学, 応用生物学部, 講師 (40397039)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 口腔生化学 / 破骨細胞 / ABCトランスポーター / Abca5 / 骨代謝

Research Abstract

The expression of nine and four of 48 ABC transporters were found to be up-and down-regulated during osteoclastgenesis, respectively. Among them, the expression of Abca5 protein was also found to be increased during osteoclastgenesis. Abca5-deficient female mice showed the possibility that Abca5 could activate the bone resorption and/or inhibit bone formation.

Research Products

• [Journal Article] Ubiquitin-mediated proteasomal degradation of ABC transporters : A new aspect of genetic polymorphisms and clinical impacts (2011)
  • Author(s): Nakagawa, H., Toyoda, Y., Wakabayashi-Nakao, K., Tamaki, H., Osumi, M., and Ishikawa, T.
  • Journal Title: J. Pharm. Sci Volume: 100 Pages: 3602-3619
  • Peer Reviewed
• [Journal Article] Enhanced expression of coproporphyrinogen oxidase in malignant brain tumors : CPOX expression and 5-ALA-induced fluorescence (2011)
  • Author(s): Takahashi, K., Ikeda, N., Nonoguchi, N., Kajimoto, Y., Miyatake, S., Hagiya, Y., Ogura, S., Nakagawa, H., Ishikawa, T., and Kuroiwa, T.
  • Journal Title: Neuro Oncol Volume: 13 Pages: 1234-1243
  • Peer Reviewed
• [Journal Article] Key Role of Human ABC Transporter ABCG2 in Photodynamic Therapy and Photodynamic Diagnosis (2011)
  • Author(s): Ishikawa, T., Nakagawa, H., Hagiya, Y., Nonoguchi, N., Miyatake, S., and Kuroiwa, T.
  • Journal Title: Advances in Pharmacological Science Volume: (in press)
  • Peer Reviewed
• [Journal Article] Production of cells with targeted integration of gene variants of human ABC transporter for stable and regulated expression using the Flp recombinase system (2010)
  • Author(s): Wakabayashi-Nakao, K., Tamura, A., Toyoda, Y., Koshiba, S., Nakagawa, H., and Ishikawa, T.
  • Journal Title: Methods Mol. Biol Volume: 648 Pages: 139-159
  • Peer Reviewed
• [Journal Article] Production of cells with targeted integration of gene variants of human ABC transporter for stable and regulated expression using the Flp recombinase system. (2010)
  • Author(s): Wakabayashi-Nakao, K., Tamura, A., Toyoda, Y., Koshiba, S., Nakagawa, H., Ishikawa, T.
  • Journal Title: Methods Mol.Biol. Volume: 648 Pages: 139-159
  • Peer Reviewed
• [Presentation] Genetic polymorphisms of human ABC transporter ABCC11 and apocrine glands (2012)
  • Author(s): Toyoda, Y., Sakurai, A., Sakai, Y., Gomi, T., Nakagawa, H., Nakashima, M., Yoshiura, K., Niikawa, N., and Ishikawa, T.
  • Organizer: The International Symposium on Past, Present and Future of Molecular Pharmacokinetics ; Integration of Basic Science, Drug Development and Regulation
  • Place of Presentation: 東京
• [Presentation] Ubiquitin-mediated proteasomal degradation of human ABC transporter ABCG2 : A new aspect of genetic polymorphisms and clinical impacts (2012)
  • Author(s): Wakabayashi-Nakao, K., Nakagawa, H., Toyoda, Y., and Ishikawa, T.
  • Organizer: The International Symposium on Past, Present and Future of Molecular Pharmacokinetics ; Integration of Basic Science, Drug Development and Regulation
  • Place of Presentation: 東京
• [Presentation] The low polar and planar structure of aminoguanidine alkaloid contributes to the interaction with human ATP-binding cassette transporter ABCB1 (2012)
  • Author(s): Nakagawa, H., Tamura, A., Li, S., Aida-Hyugaji, S., Regasini LO, Bolzani Vda S, Hirose, S., Efferth, T., Fricker, G., and Ishikawa, T.
  • Organizer: The International Symposium on Past, Present and Future of Molecular Pharmacokinetics ; Integration of Basic Science, Drug Development and Regulation
  • Place of Presentation: 東京
• [Presentation] U937細胞における抗CD98抗体依存性細胞融合阻害物質の探索 (2012)
  • Author(s): 桂川美咲, 秋山智美, 伊藤守弘, 伊藤康彦, 中川大, 永井和夫, 禹済泰, 車炳允, 飯田加賀美, 大西素子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
• [Presentation] 骨芽細胞の分化に対する月桃由来化合物の作用 (2012)
  • Author(s): 夏目矩行, 長谷川森一, 米澤貴之, 車炳允, 中川大, 照屋俊明, 永井和夫, 禹済泰
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
• [Presentation] アミノレブリン酸添加後のポルフィリン蓄積に関わるトランスポーターの探索 (2012)
  • Author(s): 松本健太郎, 萩谷裕一郎, 中川大, 石川智久, 大倉一郎, 小倉俊一郎
  • Organizer: 日本化学会
  • Place of Presentation: 東京
• [Presentation] ゲフィチニブによるCPT-11の抗腫瘍効果に対する影響 (2012)
  • Author(s): 井上裕貴, 池上洋二, 中川大, 佐野和美, 吉田久博, 石川智久
  • Organizer: 日本薬学会
  • Place of Presentation: 札幌
• [Presentation] The relationship between the function of ABCC11 and apocrine gland development in humans (2012)
  • Author(s): Toyoda, Y., Sakurai, A., Sakai, Y., Gomi, T., Nakagawa, H., Nakashima, M., Yoshiura, K., Niikawa, N., and Ishikawa, T.
  • Organizer: The FEBS-ABC2012.4th FEBS Special Meeting on ATP-Binding Cassette(ABC) Proteins : From Multidrug Resistance to Genetic Disea
  • Place of Presentation: Innsbruck
• [Presentation] The combination of isoprenyl and guanidium core structural units influences the affinity level of guanidine alkaloid for human ATP-Binding Cassette Transporter ABCB1 (2012)
  • Author(s): Nakagawa, H., Tamura, A., Li, S., Aida-Hyugaji, S., Regasini LO, Bolzani Vda S, Hirose, S., Ishikawa, T., Fricker, G., and Efferth, T.
  • Organizer: The FEBS-ABC2012.4th FEBS Special Meeting on ATP-Binding Cassette(ABC) Proteins : From Multidrug Resistance to Genetic Diseases
  • Place of Presentation: Innsbruck
• [Presentation] ヒトABCトランスポーターABCG2によるポルフィリン輸送とその阻害剤のQSAR解析 (2011)
  • Author(s): 中川大, 齊藤光, 山乙教之, 広野修一, 石川智久
  • Organizer: ポルフィリン-ALA学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-07
• [Presentation] アミノレブリン酸(5-ALA)による脳腫瘍の蛍光強度とその調節因子について (2011)
  • Author(s): 高橋賢吉, 池田直廉, 野々口直助, 梶本宜永, 宮武伸一, 萩谷祐一郎, 小倉俊一郎, 中川大, 石川智久, 黒岩敏彦
  • Organizer: ポルフィリン-ALA学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-07
• [Presentation] アミノレブリン酸を用いたポルフィリン蓄積に関わるトランスポーターの探索 (2011)
  • Author(s): 松本健太郎, 萩谷裕一郎, 中川大, 大倉一郎, 小倉俊一郎
  • Organizer: ポルフィリン-ALA学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-07
• [Presentation] 骨粗鬆症モデルマウスにおけるfargesinの骨代謝調節作用 (2011)
  • Author(s): 浅井みどり, 李永實, 中川大, 二宮禎, 小澤英浩, 山口宏二, 米澤貴之, 車炳允, 照屋俊明, 大西素子, 永井和夫, 禹済泰
  • Organizer: 日本動物細胞工学会
  • Place of Presentation: 東京
• [Presentation] 月桃(ゲットウ)由来の骨代謝調節物質の単離・精製及び生物活性 (2011)
  • Author(s): 夏目矩行, 米澤貴之, 車炳允, 中川大, 照屋俊明, 永井和夫, 禹済泰
  • Organizer: 日本動物細胞工学会
  • Place of Presentation: 東京
• [Presentation] 固相法を用いたDestruxin類縁体の全合成と生物活性評価 (2011)
  • Author(s): 吉田将人, 石田恵崇, 竹内久征, 中川大, 土井隆行
  • Organizer: 有機合成化学セミナー
  • Place of Presentation: 山形
• [Presentation] The Effect of Gefitinib on Antitumor Activity of Irinotecan (2011)
  • Author(s): Inoue, Y., Ikegami, Y., Nakagawa, H., Sano, K., Yoshida, H., and Ishikawa, T.
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
• [Presentation] Screening of transporters involved in porphyrin accumulation after administration of 5-aminolevulinic acid in vitro (2011)
  • Author(s): Matsumoto, K., Hagiya, Y., Nakagawa, H., Ishikawa, T., and Ogura, S.
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
• [Presentation] Genetic polymorphisms in human ABC transporter ABCC11 gene. (2011)
  • Author(s): Toyoda, Y., Nakagawa, H., Nakashima, M., Yoshiura, K., Niikawa, N., and Ishikawa, T.
  • Organizer: ABC Symposium 2011 in Kyoto
  • Place of Presentation: 京都
• [Presentation] The low polarity and planar structure of guanidine alkaloids contribute to their interaction with human ABC transporter ABCB1 (2011)
  • Author(s): Nakagawa, H., Tamura, A., Li, S., Aida-Hyugaji, S., Regasini LO, Bolzani Vda S, Hirose, S., Ishikawa, T., Fricker, G., and Efferth, T.
  • Organizer: ABC Symposium 2011 in Kyoto
  • Place of Presentation: 京都
• [Presentation] Ubiquitin-mediated proteasomal degradation of human ABC transporter ABCG2 : A new aspect of genetic polymorphisms and clinical impacts (2011)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Toyoda, Y., and Ishikawa, T.
  • Organizer: ABC Symposium 2011 in Kyoto
  • Place of Presentation: 京都
• [Presentation] The Effect of Gefitinib on Antitumor Activity of Irinotecan (2011)
  • Author(s): Inoue, Y., Ikegami, Y., Nakagawa, H., Sano, K., Yoshida, H., and Ishikawa, T.
  • Organizer: 日本薬物動態学会
  • Place of Presentation: 広島
• [Presentation] ゲフィチニブはABCB1およびABCG2による抗癌剤耐性のどちらをより選択的に克服するのか (2010)
  • Author(s): 井上裕貴, 池上洋二, 中川大, 佐野和美, 石川智久
  • Organizer: 日本がん分子標的治療学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-07
• [Presentation] Artesunate und seine Derivate in der Anwendung gegen die HNSCC Zelllinie(UMSCC1) (2010)
  • Author(s): Sertel, S., Hartinger, EM., Eichhorn, T., Nakagawa, H., Fricker, G., Simon, CH., Plinkert, PK., und Efferth, T.
  • Organizer: Deutsche Gesellschaft fur Hals-Nasen-Ohren-Heilkunde, Kopf-und Hals-Chirurgie e. V
  • Place of Presentation: Wiesbaden
• [Presentation] Selectivity of gefitinib to ABCB1 and ABCG2 with respect to its potential of transport inhibition and restoration of drug resistance in cancer cells (2010)
  • Author(s): Inoue, Y., Ikegami, Y., Nakagawa, H., Sano, K., Yoshida, H., and Ishikawa, T.
  • Organizer: 日本薬物動態学会
  • Place of Presentation: 大宮
• [Presentation] Genetic polymorphism of human ABCC11 as a determinant of earwax type, axillary osmidrosis, and the risk of breast cancer. (2010)
  • Author(s): 豊田優, 櫻井亜季, 太田郁子, 坂井靖夫, 五味常明, 中川大, アレキサンダーレジャバ, 中島正洋, 吉浦孝一郎, 林崎良英, 新川詔夫, 石川智久
  • Organizer: 日本人類遺伝学会
  • Place of Presentation: 大宮
• [Presentation] Detection of the SNP Q141K in the ABCG2 gene by the SmartAmp method : Molecular biological rationale and clinical applications for gout risk prediction. (2010)
  • Author(s): Wakabayashi-Nakao, K., Aw, W., Nakagawa, H., Furukawa, T., Lezhava, A., Hayashizaki, Y., and Ishikawa, T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] ERAD of genetic variants of human ABC transporters ABCG2 and ABCC11 (2010)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Toyoda, Y., Furukawa, T., Tamaki, H., Osumi, M., and Ishikawa, T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Book] ヒトABCトランスポーターのファーマコゲノミクス:迅速SNP検出法の開発.遺伝子医学MOOK (2011)
  • Author(s): 豊田優, Wanping Aw, Alexander Lezava, 中川大, 林崎良英, 石川智久
  • Publisher: 株式会社メディカルドゥ
• [Book] 小胞体におけるABCトランスポーターの品質管理-ユビキチン-プロテアソーム経路による新生ABCG2蛋白質の除去-遺伝子医学MOOK (2011)
  • Author(s): 中川大, 豊田優, 中尾(若林)香菜子, 石川智久
  • Publisher: 株式会社メディカルドゥ
• [Book] 機能性食品素材の骨と軟骨への応用 (2011)
  • Author(s): 中川大, 禹済泰.カテキン
  • Publisher: シーエムシー出版
• [Book] VI-2 ABCC11遺伝子の機能.腋臭症・多汗症治療実践マニュアル (2011)
  • Author(s): 豊田優, 中川大, 石川智久