| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Many of Rab-family GTPase and KIF-family motor proteins were expressed in osteoclast regardless of its differentiation stage. We analyzed the changes of localization of some molecules that are thought to have polarity using GFP fusion system and siRNA-knockdown system. All of the molecules we analyzed have no effects, and these results seem to show the redundancy of Rab-family proteins. 2, Quercetin, one of major flavonoids found in many fruits, inhibited the differentiation of osteoclast. The assay using specific agonist and antagonist showed that the effects of quercetin were principally via GPR30, GPCR-type estrogen receptor, and Akt phosphorylation pathway.
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