| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
We have previously shown that hypoxia down-regulated the IFN-induced expression of chemokine CXCL9 and CXCL10 genes in human tumor cell lines. We have explored the mechanisms by which hypoxia down-regulates the IFN-induced gene expressions and found that although HIF-1 was not involved in the transcriptional repression of the IFN-induced genes, recruitments of Pol II, coactivator SRC-1 were inhibited under the hypoxia. In this study, we investigated potential involvements of HIF-2, histone methylation at the promoter region of the CXCL9 genes, and mediator complex that participates in the recruitment of Pol II for the hypoxia-mediated transcriptional repression. Over expression of an active form of HIF-2 in HEK293 cells inhibited the CXCL9 promoter activity, suggesting that HIF-2 negatively regulates CXCL9 expression. Real-time RT-PCR analysis showed that constitutive expressions of mRNAs for methyltransferase and members of mediator complex were observed in HSC-2 cells.
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