| Project/Area Number |
22791798
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Research Collaborator |
KAMIJO Ryutaro 昭和大学, 歯学部, 教授 (70233939)
SUZUKI Dai 昭和大学, 歯学部, 助教 (00585797)
AIZAWA Ryo 昭和大学, 歯学部, 大学院生
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 遺伝子 / 細胞・組織 / 発生・分化 / シグナル伝達 / chondrogenesis / limb development / Rho GTPase / BMP |
|---|
| Research Abstract |
Cdc42, a member of the Rho subfamily of small GTPases, is known to be a regulator of multiple cellular functions, including cytoskeletal organization, cell migration, proliferation, and apoptosis. To investigate the physiological function of Cdc42 during limb development, we generated limb bud mesenchyme-specific inactivated Cdc42(Cdc42^<fl/fl>; Prx1-Cre) mice. Cdc42^<fl/fl>; Prx1-Cre mice demonstrated short limbs and body, abnormal calcification of the cranium, cleft palate, disruption of the xiphoid process, and syndactyly. Syndactyly in Cdc42^<fl/fl>; Prx1-Cre mice was caused by fusion of metacarpals and a failure of interdigital programmed cell death(ID-PCD). These results demonstrate that Cdc42 is essential for chondrogenesis and ID-PCD during limb development.
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