Study on the physiological roles of toll-like receptors in inflammatory bone destruction

• Project/Area Number: 22791801
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Showa University
• Principal Investigator: MOCHIZUKI Ayako 昭和大学, 歯学部, 助教 (10453648)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 骨 / 破骨細胞 / 樹状細胞 / 細胞分化 / 炎症 / 免疫 / Toll-like receptor / RANKL / 炎症性骨疾患 / 分化振り分け因子 / IRF5 / IRF8 / STAT5 / マクロファージ / 炎症性骨破壊 / 遺伝子改変マウス

Research Abstract

Identification and characterization of osteoclastogenesis regulatory factors will be useful for development of therapeutic approach for inflammatory bone diseases. We found that signal transducers and activators of transcription(STAT) 5, which is a transcription factor has the potential to play an important role for suppressing osteoclast differentiation. These results will contribute to development for bone metabolism research and medicine for inflammatory diseases.

Research Products

• [Journal Article] R848, a toll-like receptor 7 agonist, inhibits osteoclast differentiation but not survival or bone-resorbing function of mature osteoclasts (2012)
  • Author(s): Miyamoto A, Takami M, Matsumoto A, Mochizuki A, Yamada T, Tachi K, Shibuya I, Nakamachi T, Shioda S, Baba K, Inoue T, Miyamoto Y, Yim M, Kamijo R
  • Journal Title: Cytotechnology
• [Journal Article] R848, a toll-like receptor 7 agonist, inhibits osteoclast differentiation but not survival or bone-resorbing function of mature (2012)
  • Author(s): Miyamoto A, Takami M, Matsumoto A, Mochizuki A, Yamada T, Tachi K, Shibuya I, Nakamachi T, Shioda S, Baba K, Inoue T, Miyamoto Y, Yim M
  • Journal Title: Cytotechnology Volume: (印刷中)(取得中)
  • Peer Reviewed
• [Journal Article] Decreased Intake of Sucrose Solutions in Orexin Knockout Mice (2011)
  • Author(s): Matsuo E, Mochizuki A, Nakayama K, Nakamura S, Yamamoto T, Shioda S, Sakurai T, Yanagisawa M, Shiuchi T, Minokoshi Y, Inoue T
  • Journal Title: J Mol Neurosci Volume: 43 Pages: 217-224
  • Peer Reviewed
• [Journal Article] Enhancement of bone morphogenetic protein-2-induced ectopic bone formation by transforming growth factor-β1 (2011)
  • Author(s): Tachi K, Takami M, Sato H, Mochizuki A, Zhao B, Miyamoto Y, Tsukasaki H, Inoue T, Shintani S, Koike T, Honda Y, Suzuki O, Baba K, Kamijo R
  • Journal Title: Tissue Eng Part A Volume: 17 Pages: 597-606
  • Peer Reviewed
• [Journal Article] Decreased Intake of Sucrose Solutions in Orexin Knockout Mice. (2011)
  • Author(s): Mochizuki A, Matsuo E, Nakayama K, Nakamura S, Yamamoto T, Shioda S, Sakurai T, Yanagisawa M, Shiuchi T, Minokoshi Y, Inoue T
  • Journal Title: Journal of Molecular Neuroscience Volume: 43(2) Pages: 217-224
  • Peer Reviewed
• [Journal Article] Enhancement of bone morphogenetic protein-2-induced ectopic bone formation by transforming growth factor-β1. (2011)
  • Author(s): Tachi K, Takami M, Sato H, Mochizuki A, Zhao B, Miyamoto Y, Tsukasaki H, Inoue T, Shintani S, Koike T, Honda Y, Suzuki O, Baba K, Kamijo R.
  • Journal Title: Tissue Engineering Part A. Volume: 17(5-6) Pages: 597-606
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein 2 enhances mouse osteoclast differentiation via increased levels of receptor activator of NF-kB ligand expression in osteoblasts (2010)
  • Author(s): Tachi K, Takami M, Zhao B, Mochizuki A, Yamada A, Miyamoto Y, Inoue T, Baba K, Kamijo R
  • Journal Title: Cell Tissue Res Volume: 342(2) Pages: 213-220
  • Peer Reviewed
• [Journal Article] Properties of synaptic transmission from the reticular formation dorsal to the facial nucleus to trigeminal motoneurons during early postnatal development in rats (2010)
  • Author(s): Gemba-Nishimura A, Inoue T, Nakamura S, Nakayama K, Mochizuki A, Shintani S, Yoshimura S
  • Journal Title: Neuroscience Volume: 166 Pages: 1008-1022
  • Peer Reviewed
• [Journal Article] Involvement of transcription factor IRF-8 in bone metastasis of cancer (2010)
  • Author(s): Kamijo R, Zhao B, Mochizuki A, Takami M
  • Journal Title: The Waksman Foundation of Japan, Tokyo
• [Journal Article] Bone morphogenetic protein 2 enhances mouse osteoclast differentiation via increased levels of receptor activator of NF-κB ligand expression in osteoblasts. (2010)
  • Author(s): Tachi K, Takami M, Zhao B, Mochizuki A, Yamada A, Miyamoto Y, Inoue T, Baba K, Kamijo R
  • Journal Title: Cell and Tissue Research Volume: 342(2) Pages: 213-220
  • Peer Reviewed
• [Presentation] 蔗糖溶液摂取におけるOrexinの関与 (2011)
  • Author(s): 望月文子、松尾英子、中山希世美、中村史朗、井上富雄
  • Organizer: 第5回三叉神経領域の感覚-運動統合機構研究会
  • Place of Presentation: 長野県・松本
  • Year and Date: 2011-12-03
• [Presentation] 三叉神経運動ニューロンには三叉神経運動核周囲の様々な部位からの入力が収束する (2011)
  • Author(s): 野中睦美、西村晶子、中山希世美、中村史朗、望月文子、飯島毅彦、井上富雄
  • Organizer: 日本顎口腔機能学会第47回学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-10-22
• [Presentation] 三叉神経運動ニューロンには三叉神経運動核周囲の様々な部位からの入力が収束する (2011)
  • Author(s): 野中睦美、西村晶子、中山希世美、中村史朗、望月文子、飯島毅彦、井上富雄
  • Organizer: 日本顎口腔機能学会第47回学術大会
  • Place of Presentation: 兵庫県・神戸
  • Year and Date: 2011-10-22
• [Presentation] Properties of synaptic transmission from regions surrounding the trigeminal motor nucleus to trigeminal motoneurons in neonatal rats (2011)
  • Author(s): Nonaka M, Inoue T, Nishimura A, NakayamaK, Mochizuki A, Nakamura S, Yoshimura S, Iijima T
  • Organizer: 8th IBRO world congress of neuroscience Florence
  • Year and Date: 2011-07-18
• [Presentation] Properties of synaptic transmission from regions surrounding the trigeminal motor nucleus to trigeminal motoneurons in neonatal rats (2011)
  • Author(s): Nonaka M, Inoue T, Nishimura A, Nakayama K, Mochizuki A, Nakamura S, Yoshimura S, Iijima T
  • Organizer: 8^<th> IBRO world congress of neuroscience
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-07-18
• [Presentation] Orexin knockout miceは蔗糖溶液摂取量が減少する (2011)
  • Author(s): 望月文子、松尾英子、中山希世美、中村史朗、井上富雄
  • Organizer: 日本顎口腔機能学会第46回学術大会
  • Place of Presentation: 仙台
  • Year and Date: 2011-05-29
• [Presentation] Orexin knockout miceは蔗糖溶液摂取量が減少する (2011)
  • Author(s): 望月文子、松尾英子、中山希世美、中村史朗、井上富雄
  • Organizer: 日本顎口腔機能学会第46回学術大会
  • Place of Presentation: 宮城県・仙台
  • Year and Date: 2011-05-29
• [Presentation] 顎運動制御機構におけるプレモーターニューロンの形態学的および電気生理学的解析 (2011)
  • Author(s): 中村史朗、望月文子、中山希世美、井上富雄
  • Organizer: 昭和大学大学院歯学研究科・口腔癌包括的研究センター平成22年度合同シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-03-26
• [Presentation] 口腔癌と免疫システムの相互作用の解明 (2011)
  • Author(s): 宮本阿礼、高見正道、望月文子、井上富雄、上條竜太郎
  • Organizer: 昭和大学大学院歯学研究科・口腔癌包括的研究センター平成22年度合同シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-03-26
• [Presentation] Decreased sucrose intake and locomotor activity in orexin knockout mice (2011)
  • Author(s): Matsuo E, Mochizuki A, Nakayama K, Nakamura S, Yamamoto T, Shioda S, Sakurai T, Inoue T
  • Organizer: the 89th General Session & Exhibition of the International Association for Dental Research Meeting Location
  • Place of Presentation: San Diego
  • Year and Date: 2011-03-19
• [Presentation] Decreased sucrose intake and locomotor activity in orexin knockout mice (2011)
  • Author(s): Matsuo E, Mochizuki A, Nakayama K, Nakamura S, Yamamoto T, Shioda S, Sakurai T, Inoue T
  • Organizer: the 89th General Session & Exhibition of the International Association for Dentat Research Meeting Location
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-19
• [Presentation] Electrophysiological and morphological properties of premotor neurons targeting jaw-closing and jaw-opening motoneurons (2011)
  • Author(s): Inoue T, Nakamura S, Gemba-Nishimura A, Nonaka M, Yoshida A, Mochizuki A, Nakayama K
  • Organizer: NANOSYMPOSIUM ; Orofacial Systems : Respiration, Communication, and Feeding ; Theme D : Sensory and Motor Systems, Neuroscience
  • Place of Presentation: Washington, DC
• [Presentation] 新生マウス脳幹摘出標本におけるリズミックな顎運動の誘発 (2010)
  • Author(s): 中山希世美、望月文子、西村晶子、井上富雄
  • Organizer: 日本顎口腔機能学会第45回学術大会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-11-06
• [Presentation] 新生マウス 脳幹摘出標本におけるリズミックな顎運動の誘発 (2010)
  • Author(s): 中山希世美、望月文子、西村晶子、井上富雄
  • Organizer: 日本顎口腔機能学会 第45回学術大会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-11-06
• [Presentation] 三叉神経中脳路核ニューロンにおけるオレキシンの作用 (2010)
  • Author(s): 中山希世美、望月文子、井上富雄
  • Organizer: 第7回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-05-11
• [Presentation] 三叉神経中脳路核ニューロンにおけるオレキシンの作用 (2010)
  • Author(s): 中山希世美、望月文子、井上富雄
  • Organizer: 第7回 GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-05-11
• [Book] Involvement of transcription factor IRF-8 in bone metastasis of cancer. (2010)
  • Author(s): kamijo R, Zhao B, Mochizuki A, Inoue T, Takami M
  • Total Pages: 48
  • Publisher: The Waksman Foundation of Japan.
• [Remarks] 昭和大学歯学部口腔生理学ホームページ
  • URL: http://www.showa-u.ac.jp/sch/dent/major/oralphys/index.html
• [Remarks]
  • URL: http://www10.showa-u.ac.jp/~oralphys/