| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Research Abstract |
In order to visualize secretory granules in rat parotid acinar cells, we incubated the cells with a fluorescent dye for 3 hours after exocytosis. Intracellular localization of the dye into secretory granules was confirmed by morphological, biochemical and functional study. Moreover immature secretory granules including the dye matured to large after 2 days cell culture. These results expect that examination of mechanisms of granule maturation in rat parotid granules.
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