Visualization of secretory granules in rat parotid gland, and examination of mechanisms of granule maturation.
| Project/Area Number |
22791803
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
KATO Osamu (KATSUMATA Osamu) 日本大学, 松戸歯学部, 講師 (70349968)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 歯学 / 生理学 / シグナル伝達 / 唾液腺 / 分泌顆粒 / エンドサイトーシス / 耳下腺 / 可視化 / 初代培養系 / ペリフュージョン / ルシファーイエロー |
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| Research Abstract |
In order to visualize secretory granules in rat parotid acinar cells, we incubated the cells with a fluorescent dye for 3 hours after exocytosis. Intracellular localization of the dye into secretory granules was confirmed by morphological, biochemical and functional study. Moreover immature secretory granules including the dye matured to large after 2 days cell culture. These results expect that examination of mechanisms of granule maturation in rat parotid granules.
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Report
(4 results)
Research Products
(11 results)
