The role of acid sensing nociceptors in the development of cancer-induced bone pain

• Project/Area Number: 22791811
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Osaka Medical College (2011); Osaka University (2010)
• Principal Investigator: NAKANISHI Masako 大阪医科大学, 医学部, 助教 (60437382)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 骨転移 / 癌性疼痛 / 酸感受性受容体 / 動物モデル

Research Abstract

The pH-sensitive fluorescence dye indicated that acidic microenvironment was created in bone metastases of rats. It was suggested that acidic microenvironment activate acid sensing nociceptors in the sensory neurons, which in turn leads to up-regulation of CGRP expression. These events may be involved in the pathophysiology of bone pain.

Research Products

• [Journal Article] The a3 isoform vacuolar type H+-ATPase promotes distant metastasis in the mouse B16 melanoma cells (2011)
  • Author(s): Nishisho T, Hata K, Nakanishi M, Morita Y, Sun-Wada H, Wada Y, Yasui N, Yoneda T
  • Journal Title: Mol Cancer Res Volume: 9 Pages: 845-855
• [Journal Article] Encounter of cancer cells with bone. Molecular mechanism of cancer-induced bone pain (2011)
  • Author(s): Nakanishi M, Hata K, Yoneda T
  • Journal Title: Clin Calcium Volume: 21 Pages: 357-362
• [Journal Article] Encounter of cancer cells with bone. The bone microenvironment and cancer metastasis (2011)
  • Author(s): Hata K, Nakanishi M, Morita Y, Yoneda T
  • Journal Title: Clin Calcium Volume: 21 Pages: 329-355
• [Journal Article] Involvement of acidic microenvironment in the pathophysiology of cancer-associated bone pain (2011)
  • Author(s): Yoneda T, Hata K, Nakanishi M, Nagae M, Nagayama T, Wakabayashi H, Nishisho T, Sakurai T and Hiraga T
  • Journal Title: Bone Volume: 48 Pages: 100-105
• [Journal Article] 骨転移に伴うがん性骨痛の分子メカニズム (2011)
  • Author(s): 中西雅子、波多賢二、米田俊之
  • Journal Title: CLINICAL CALCIUM Volume: 21 Pages: 357-363
• [Journal Article] Acid activation of Trpv1 leads to an up-regulation of calcitonin gene related peptide expression in dorsal root ganglion neurons via the CaMK-CREB cascade : A potential mechanism of inflammatory pain (2010)
  • Author(s): Nakanishi M, Hata K, Nagayama T, Sakurai T, Nishisho T, Wakabayashi H, Hiraga T, Ebisu S and Yoneda T
  • Journal Title: Mol. Biol. Cell Volume: 21 Pages: 2568-2577
• [Journal Article] Acid activation of Trpv1 leads to an up-regulation of CGRP expression in DRG neurons via the CaMK-CREB cascade : a potential mechanism of inflammatory pain. (2010)
  • Author(s): Masako Nakanishi
  • Journal Title: Mol Biol Cell Volume: 21 Pages: 2568-2577
  • Peer Reviewed
• [Presentation] Involvement of acidic microenvironment on the cancer-induced bone pain (2012)
  • Author(s): Masako Nakanishi, Kenji Hata, Toshiyuki Yoneda
  • Organizer: 第117回日本解剖学会
  • Place of Presentation: 甲府
  • Year and Date: 2012-03-28
• [Presentation] Up-regulation of CGRP expression by acid-activated calcium channel nociceptor Trpv1 is involved in bone pain (2011)
  • Author(s): 中西雅子、波多賢二、永山智崇、西庄俊彦、米田俊之
  • Organizer: 第5回Bone Research Seminar
  • Place of Presentation: 東京
  • Year and Date: 2011-02-19
• [Presentation] Up-regulation of CGRP expression by acid-activated calcium channel nociceptor Trpv1 is involved in bone pain. (2011)
  • Author(s): 中西雅子
  • Organizer: 第5回Bone Research Seminar
  • Place of Presentation: 東京コンファレンスセンター(招待講演)
  • Year and Date: 2011-02-19
• [Presentation] Up-regulation of CGRP expression by acid-activated calcium channel nociceptor Trpv1 is involved in bone pain (2010)
  • Author(s): Masako Nakanishi, Kenji Hata, Tomotaka Nagayama, Toshihiko Nishisho, Toshiyuki Yoneda
  • Organizer: The American Society for Bone and Mineral Research 2010 Annual Meeting
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2010-10-18
• [Presentation] Up-regulation of CGRP expression by acid-activated calcium channel nociceptor Trpv1 is involved in bone pain. (2010)
  • Author(s): Masako Nakanishi
  • Organizer: The American Society for Bone and Mineral Research 2010 Annual Meeting
  • Place of Presentation: Metro Toronto Convention Center(Canada)
  • Year and Date: 2010-10-18
• [Presentation] 骨転移巣の酸性環境はTRPV1の活性化によるCGRPの発現増加を介して疼痛に関与する (2010)
  • Author(s): 中西雅子、米田俊之
  • Organizer: 第69回日本癌学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] Acid microenvironment in bone metastases is involved in pain signal through CGRP regulation following Trpv1 activation. (2010)
  • Author(s): Masako Nakanishi
  • Organizer: 第69回日本癌学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-22
• [Presentation] 骨転移巣の酸性環境は知覚神経TRPV1の活性化によるCGRPの発現増加を介して疼痛を誘発する (2010)
  • Author(s): 中西雅子、波多賢二、西庄俊彦、米田俊之
  • Organizer: 第28回日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-23
• [Presentation] 骨転移巣の酸性環境は知覚神経TRPV1の活性化によるCGRPの発現増加を介して疼痛を誘発する (2010)
  • Author(s): 中西雅子
  • Organizer: 第28回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2010-07-23