Search of evidence on the effect with dental resin curing light irradiation against dental pulp

• Project/Area Number: 22791848
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: YOSHINO Fumihiko 神奈川歯科大学, 歯学部, 准教授 (20308307)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 光照射器 / 活性酸素 / 青色光 / 酸化ストレス / アポトーシス / レジン光照射器 / 活性酸素種 / 抗酸化剤 / ハロゲン光 / 歯科用可視光照射器 / 血管収縮 / ノルアドレナリン / 一重項酸素 / ヒドロキシルラジカル / スーパーオキシド

Research Abstract

Dental resin curing light has been used to restore of tooth with resin-based composite fillings and to treat of tooth bleaching. However, it has not been less considered the effect on the oral tissues such as dental pulp or oral mucosa. The aim of this study was to investigate whether dental resin curing light irradiation is affected on the dental pulp. We found that irradiation of resin curing blue light was increased the level of lipid peroxidation on isolated rat aorta blood vessels to use the measurement of malondialdehyde. Furthermore, it was decreased the cell proliferation activity in time-dependent manner and was induced the apoptosis of human aorta vascular smooth muscle cells(VSMC). Therefore, these results indicated that reactive oxygen species such as hydrogen peroxide and hydroxyl radical were generated in VSMC by blue light irradiation, and they were induced the cytotoxicity associated with oxidative stress which was increased the lipid peroxidation and apoptosis. In addition, N-acetyl-L-cysteine which is typical intracellular antioxidants prevented VSMC from cytotoxicity associated with oxidative stress.

Research Products

• [Journal Article] Crocetin reduces the oxidative stress induced reactive oxygen species in the SHRSPs brain (2011)
  • Author(s): Yoshino F., Yoshida A., Umigai N., Kubo K., Lee M-C.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 49(3) Pages: 182-187
  • Peer Reviewed
• [Journal Article] Soft-food diet induces oxidative stress in the rat brain (2011)
  • Author(s): Ohno A., Yoshino F., Yoshida A., Hori N., Ono Y., Kimoto K., Onozuka M., Lee M-C.
  • Journal Title: Neuroscience Letters Volume: 508 Pages: 42-46
  • Peer Reviewed
• [Journal Article] Crocetin reduces the oxidative stress induced reactive oxygen species in the stroke-prone spontaneously hypertensive rats (SHRSPs) brain (2011)
  • Author(s): Yoshino F, Yoshida A., Umigai N., Kubo K., Lee M-C.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 49 Issue: 3 Pages: 182-187
  • DOI: 10.3164/jcbn.11-01
  • NAID: 130004466653
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Assessment of Anti-Aging Effects of Fish Products Peptide in Middle-Aged Subjects (2011)
  • Author(s): Yoshino F., Yoshida A., Sugiyama S., Tokutomi F., Miyamoto C., Maehata Y., Kobayashi K., Wada-Takahashi S., Maetani T., Okada E., Okada Y., Komatsu T., Takahashi S-S., Wan J., Lee M-C.
  • Journal Title: Anti-Aging Medicine Volume: 8 Pages: 48-52
  • NAID: 130000968342
  • Peer Reviewed
• [Journal Article] Direct Assessment of the Antioxidant Properties of Midazolam by Electron Spin Resonance Spectroscopy (2011)
  • Author(s): Hata M., Kobayashi K., Yoshino F., Yoshida A., Sugiyama S., Miyamoto C., Tokutomi F., Maehata Y., Wada-Takahashi S., Takahashi S-S., Komatsu T., Yoshida K., Lee M-C.
  • Journal Title: Journal of Anesthesia Volume: 25 Issue: 5 Pages: 765-769
  • DOI: 10.1007/s00540-011-1184-6
  • NAID: 10031137045
  • Peer Reviewed
• [Journal Article] Direct assessment by electron spin resonance spectroscopy of the antioxidant effects of French maritime pine bark extract in the maxillofacial region of hairless mice (2011)
  • Author(s): Yoshida A., Yoshino F., Tsubata M., Ikeguchi M., Nakamura T., Lee M-C.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 49 Issue: 2 Pages: 79-86
  • DOI: 10.3164/jcbn.10-103
  • NAID: 130004466650
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy (2011)
  • Author(s): Kobayashi K., Maehata Y., Kawamura Y., Kusubata M., Hattori S., Tanaka K., Miyamoto C., Yoshino F., Yoshida A., Wada-Takahashi S., Komatsu T., Takahashi S-S., Lee M-C.
  • Journal Title: Journal of Pharmacological Sciences Volume: 116 Issue: 1 Pages: 97-106
  • DOI: 10.1254/jphs.10321FP
  • NAID: 10029894130
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Electrostatic control of protein adsorption on UV-photofunctionalized titanium. (2010)
  • Author(s): Hori N, Ueno T, Minamikawa H, Iwasa F, Yoshino F, Kimoto K, Lee MC, Ogawa T
  • Journal Title: Acta Biomateial Volume: 6 Pages: 4175-4180
  • Peer Reviewed
• [Journal Article] Reactive Oxygen Species (ROS) Reduce the Expression of BRAK/CXCL14in Human Head and Neck Squamous Cell Carcinoma Cells. (2010)
  • Author(s): Maehata Y, Ozawa S, Kobayashi K, Kato Y, Yoshino F, Miyamoto C, Izukuri K, Kubota E, Hata R-I, Lee M-C
  • Journal Title: Free Radical Research Volume: 44 Pages: 913-924
  • Peer Reviewed
• [Journal Article] ESR detection of ROS generated by TiO2 coated with fluoridated apatite. (2010)
  • Author(s): Sawada T, Yoshino F, Kimoto K, Takahashi Y, Shibata T, Hamada N, Sawada T, Toyoda M, Lee M-C
  • Journal Title: Journal of Dental Research Volume: 89 Pages: 848-853
  • Peer Reviewed
• [Journal Article] Assessments of salivary antioxidant activity using electron spin resonance spectroscopy
  • Author(s): Yoshino F., Yoshida A., Wada-Takahashi S., Sugiyama S., Tokutomi F., Maehata Y., Miyamoto C., Komatsu T., Takahashi SS., Kobayashi K., Lee M-C.
  • Journal Title: Archives of Oral Biology Volume: (in press)
  • Peer Reviewed
• [Presentation] 歯科用レジン照射器の青色光が血管平滑筋へ及ぼす影響の検討 (2012)
  • Author(s): 吉田彩佳, 吉野文彦, 前畑洋次郎, 高橋俊介, 高橋聡子, 小林杏, 宮本千央, 徳富文彬, 李昌一
  • Organizer: 神奈川歯科大学学会第137回例会
  • Place of Presentation: 神奈川歯科大学(横須賀)
  • Year and Date: 2012-01-12
• [Presentation] 歯科用レジン照射器の青色光が血管平滑筋へ及ぼす影響の検討 (2012)
  • Author(s): 吉田彩佳, 吉野文彦, 前畑洋次郎, 高橋俊介, 高橋聡子, 小林杏, 宮本千央, 徳富文彬, 李昌一
  • Organizer: 神奈川歯科大学学会第137回例会
  • Place of Presentation: 横須賀
• [Presentation] 歯科用可視光線照射器による光照射の血管平滑筋に対する影響 (2011)
  • Author(s): 吉野文彦, 吉田彩佳, 西村知子, 石井信之, 李昌一
  • Organizer: 第135回日本歯科保存学会秋季大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] 歯科用可視光線照射器による光照射の血管平滑筋に対する影響 (2011)
  • Author(s): 吉野文彦, 吉田彩佳, 西村知子, 石井信之, 李昌一
  • Organizer: 第135回日本歯科保存学会2011年度秋季学術大会
  • Place of Presentation: 大阪国際交流センター(大阪)
  • Year and Date: 2011-10-21
• [Presentation] 歯周疾患に関わる酸化ストレスとサプリメンテーションによる予防効果 (2011)
  • Author(s): 吉野文彦
  • Organizer: 第11回日本抗加齢医学会総会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2011-05-29
• [Presentation] 新規コラーゲンペプチドは紫外線照射によって引き起こされる細胞障害を抑制する (2011)
  • Author(s): 小林杏, 前畑洋次郎, 宮本千央, 徳富文彬, 杉山秀太, 吉田彩佳, 吉野文彦, 高橋聡子, 前谷崇志, 川村陽介, 辻村傑, 岡田永三, 岡田康江, 西村成文, 小松知子, 高橋俊介, 李昌一
  • Organizer: 第11回日本抗加齢医学会総会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2011-05-28
• [Presentation] アンチエイジングサプリメントの抗酸化能評価～電子スピン共鳴(ESR)法による～ (2010)
  • Author(s): 吉野文彦
  • Organizer: 食品開発展2010
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 2010-10-14
• [Presentation] アンチエイジングサプリメントの抗酸化能評価と臨床評価の実際 (2010)
  • Author(s): 吉野文彦
  • Organizer: 第10回国際バイオEXPO
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 2010-07-01
• [Presentation] 二酸化チタン光触媒を応用した新規歯牙漂白剤の開発 (2010)
  • Author(s): 吉野文彦
  • Organizer: 第63回日本酸化ストレス学会学術集会
  • Place of Presentation: 横浜県民センター(神奈川)
  • Year and Date: 2010-06-24
• [Remarks]
  • URL: http://www.kdcnet.ac.jp/college/medicine/yakuri/yakuri.htm