Development of Cementum/PDL complex using human neural crest derived stem cells from PDL.

• Project/Area Number: 22791875
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Prosthetic dentistry
• Research Institution: Niigata University
• Principal Investigator: KAKU Masaru 新潟大学, 医歯学総合病院, 講師 (30547542)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 神経堤幹細胞 / 間葉系幹細胞 / 歯根膜 / 再生 / ニューロスフィア法

Research Abstract

The purpose of this study was to characterize the neural crest(NC) derived cells in adult periodontal ligament by using NC-traceable transgenic mice. Our study reveled that the distribution and prevalence of NC derived cells in adult PDL. Furthermore, the distribution of NC stem cell marker and mesenchymal stem cell marker positive cells were distinct.

Research Products

• [Journal Article] Identification and Characterization of Neural Crest-derived Cells in Adult Periodontal Ligament of Mice (2012)
  • Author(s): Kaku M, Komatsu Y, Mochida Y, Yamauchi M, Mishina Y, Ko CC
  • Journal Title: Arch Oral Biol Volume: (in press)
• [Journal Article] Analysis of patients visiting Niigata Medical and Dental Hospital with chief complaints of dental metal allergy and/or dental focal infection in the previous 8 years (2011)
  • Author(s): Akiba Y, Tomizuka K, Kaku M, Kawasaki M, Nagasawa M, Takano R, Uoshima K
  • Journal Title: The Indonesian Journal of Dental Research Volume: 1(2)(in press)
• [Journal Article] Analysis of Patients Visiting Niigata University Medical and Dental Hospital with Chief Complaints of Dental Metal Allergy and/or Dental Focal Infection in the Previous 8 Years (2011)
  • Author(s): Y.Akiba, K.Tomizuka, M.Kaku, et.al.
  • Journal Title: The Indonesian Journal of Dental Research Volume: Vol.1-2
  • Peer Reviewed
• [Presentation] Physiological importance of HSP27 expression in osteoblastic cells (2011)
  • Author(s): Nozawa M, Kaku M, Rocavado JM Rosales, Uoshima K
  • Organizer: International Symposium on Oral Health Education and Research
  • Place of Presentation: Balikpapan, Indonesia
  • Year and Date: 2011-12-10
• [Presentation] Osteoblastic Differentiation and Mineralization Ability of Periosteum Derived Cells in vitro (2011)
  • Author(s): Rocavado JM Rosales, Kaku M, Nozawa M, Akiba Y, Uoshima K
  • Organizer: 新潟歯学会第2回例会
  • Place of Presentation: 新潟
  • Year and Date: 2011-11-12
• [Presentation] Localization of Type XII Collagen and Its Interacting Molecules in PDL at Normal and Hyper Ocdusal Condition (2011)
  • Author(s): Kaku M, Nozawa M, Rocavado JM Rosales, Uoshima K
  • Organizer: The 7^<th> Biennial Meeting of Asian Academy of Prosthodontics
  • Place of Presentation: 上海、中国
  • Year and Date: 2011-10-30
• [Presentation] Gene expression of HSP27 During the course of osteoblast differentiation and mineralization (2011)
  • Author(s): Nozawa M, Kaku M, Rocavado JM Rosales, Uoshima K
  • Organizer: The 7^<th> Biennial Meeting of Asian Academy of Prosthodontics
  • Place of Presentation: 上海、中国
  • Year and Date: 2011-10-30
• [Presentation] 骨芽細胞分化過程におけるSmall Heat Shock Proteinの発現 (2011)
  • Author(s): 野澤恩美,加来賢, Rocavado JM Rosales,魚島勝美
  • Organizer: 第120回日本補綴歯科学会
  • Place of Presentation: 広島
  • Year and Date: 2011-05-21
• [Presentation] 歯根膜におけるXII型コラーゲンの局在と石灰化能への影響 (2011)
  • Author(s): 加来賢, Rocavado JM Rosales,野澤恩美,魚島勝美
  • Organizer: 第120回日本補綴歯科学会
  • Place of Presentation: 広島
  • Year and Date: 2011-05-20
• [Presentation] 加来賢,Rocavado JM Rosales,野澤恩美,魚島勝美,歯根膜におけるXII型コラーゲンの局在と石灰化能への影響 (2011)
  • Author(s): 加来賢, Rocavado JM Rosales, 野澤恩美, 魚島勝美
  • Organizer: 第120回日本補綴歯科学会
  • Place of Presentation: 広島
  • Year and Date: 2011-05-20
• [Presentation] COL12A1 Gene Silencing Enhances Osteoblastic Differentiation of Human PDL Cells (2011)
  • Author(s): M.KAKU, M.ROSALES, et.al.
  • Organizer: International Association for Dental Research
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-17
• [Presentation] COL12A1 Gene Silencing Enhances Osteoblastic Differentiation of Human PDL Cells (2011)
  • Author(s): Kaku M., Rosales JM., Akiba Y., Nozawa M., Uoshima K.
  • Organizer: IADR/AADR 89th General Session
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-16
• [Presentation] コラーゲン修飾酵素の特異的発現が機械的刺激による歯根膜組織の安定化を制御する (2011)
  • Author(s): 加来賢, 魚島勝美
  • Organizer: 歯科医学を中心とした総合的な研究を推進する集い
  • Place of Presentation: 東京
  • Year and Date: 2011-01-08
• [Presentation] Localization of Type XII Collagen and Its Interacting Molecules in PDL at Normal and Hyper Occlusal Condition (2011)
  • Author(s): Kaku M, Nozawa M, Rocavado JM Rosales, Uoshima K
  • Organizer: The 7^<th> Biennial Meeting of Asian Academy of Prosthodontics
  • Place of Presentation: Shanghai, China
• [Presentation] Gene expression of HSP27 During the course of osteoblast differentiation and mineralization (2011)
  • Author(s): Nozawa M, Kaku M, Rocavado JM Rosales, Uoshima K
  • Organizer: The 7^<th> Biennial Meeting of Asian Academy of Prothodontics
  • Place of Presentation: Shanghai, China
• [Presentation] Type XII Collagen Modulates Matrix Formation and Mineralization on Human PDL Cells (2010)
  • Author(s): Kaku M., Rosales JM., Akiba Y., Nozawa M., Uoshima K.
  • Organizer: International Joint Symposium on Oral Science
  • Place of Presentation: Bali, INDONESIA
  • Year and Date: 2010-12-17
• [Presentation] Mechanical Loading Induces the MMP-13 induction at Epithelial Rests of Malassez (2010)
  • Author(s): Kawasaki M, Kaku M, Rocavado JM Rosales, Nozawa M, Uoshima K.
  • Organizer: International Joint Symposium on Oral Science
  • Place of Presentation: Bali, INDONESIA
  • Year and Date: 2010-12-17
• [Presentation] Analysis Of Patients Visiting Niigata University Medical And Dental Hospital With Chief Complaints Of Dental Metal Allergy And/Or Dental Focal Infection In The Previous 8 Years (2010)
  • Author(s): Y.Akiba, K.Tomizuka, M.Kaku, M., et.al.
  • Organizer: International Joint Symposium on Oral Science
  • Place of Presentation: Bali, INDONESIA
  • Year and Date: 2010-12-17
• [Presentation] Hyper-Occlusal Loading Affects to the Expression of Collagen Modifying Enzymes (2010)
  • Author(s): Kaku M., Kawasaki M., Rosales JM., Uoshima K.
  • Organizer: JADR 58th General Session
  • Place of Presentation: 北九州
  • Year and Date: 2010-11-20
• [Presentation] Hyper-Occlusal Loading Affects to the Expression of Collagen Modifying Enzymes (2010)
  • Author(s): M.KAKU, M.KAWASAK, et.al.
  • Organizer: Japanese Association for Dental Research
  • Place of Presentation: 北九州
  • Year and Date: 2010-11-20
• [Presentation] Effect of Mechanical Loading on Post-translational Modifications of Collagen (2010)
  • Author(s): M.KAKU, M.KAWASAKI, et.al
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2010-07-17
• [Presentation] Mineralization Ability of Periosteum Derived Cells (2010)
  • Author(s): J.M.ROSALES, M.KAKU, et.al.
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2010-07-17
• [Presentation] Dental Follicle Cells Contribute to Tooth Eruption via MMP-13 Synthesis (2010)
  • Author(s): M.KAWASAKI, M.KAKU, et.al.
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2010-07-17
• [Presentation] Effect of HDACIs on mesenchymal stem cells in osteogenic differentiation (2010)
  • Author(s): Y.AKIBA, M.KAKU, M, et.al.
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2010-07-17
• [Presentation] Effect of Mechanical Loading on Post-translational Modifications of Collagen (2010)
  • Author(s): Kaku M., Kawasaki M., Rocavado JM Rosales., Uoshima K.
  • Organizer: IADR/AADR 88th General Session
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2010-07-15
• [Presentation] Effect of HDACIs on mesenchymal stem cell in osteogenic differentiation (2010)
  • Author(s): Akiba Y., Kaku M., Nagasawa M., Bhuiyan A., Uoshima K.
  • Organizer: IADR/AADR 88th General Session
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2010-07-15
• [Presentation] Detection of MMP-13 among Dental Follicle Cells during tooth eruption. (2010)
  • Author(s): Kawasaki M., Kaku M., Rashid Md. M., Uoshima K.
  • Organizer: IADR/AADR 88th General Session
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2010-07-15
• [Presentation] コラーゲン修飾酵素の特異的発現が機械的刺激による歯根膜組織の安定化を制御する (2010)
  • Author(s): 加来賢, 川崎真依子, 秋葉陽介, 他
  • Organizer: 第119回日本補綴歯科学会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-12
• [Presentation] 咀嚼能率検査とQOLアンケートによる固定性インプラント義歯と部分床義歯の比較 (2010)
  • Author(s): 川崎真依子, 吉田恵子, 加来賢, 他
  • Organizer: 第119回日本補綴歯科学会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-12
• [Presentation] 間葉系幹細胞の骨形成系誘導におけるヒストン脱アセチル化阻害剤の影響について (2010)
  • Author(s): 秋葉陽介, アルアミン ブイヤン, 川崎真依子, 加来賢, 他
  • Organizer: 第119回日本補綴歯科学会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-12
• [Presentation] 機械的刺激による骨組織の安定化機構-コラーゲン修飾酵素によるクロスリンクの生成- (2010)
  • Author(s): 加来賢, 魚島勝美
  • Organizer: 第3回 Niigata Bone Research Club
  • Place of Presentation: 新潟
  • Year and Date: 2010-05-21
• [Remarks]
  • URL: http://researchers.adm.niigata-u.ac.jp/rs/staff/?userId=100000208&lang=