Development of cell-based skeletal muscle graft materials for the tongue and masticatory muscle tissue engineering

• Project/Area Number: 22791879
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Prosthetic dentistry
• Research Institution: Osaka University
• Principal Investigator: KOBAYASHI Munemasa 大阪大学, 歯学部附属病院, 医員 (70467588)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 歯科補綴学一般 / 幹細胞 / 組織工学 / 骨格筋 / 周期的伸展刺激 / 骨格筋再生 / バイオリアクター / 骨格筋移植体

Research Abstract

The skeletal myogenesis of bone marrow-derived mesenchymal stem cells(BMSCs) offers great therapeutic promise. This research project proposes a simple method for accelerating the skeletal myogenesis of BMSCs with an aligned structure by applying cyclic strain. In addition, increasing the amplitude of the stretching device after cell alignment allowed us to obtain aligned cells as a cell sheet. This simple method based on cyclic strain may facilitate the development of a cell-based implant material that displays an in vivo-like pattern of organization.