Enhancement of biocompatibility of beta-tricalcium phosphate bone substitute by anti-oxidant amino acid derivative

• Project/Area Number: 22791903
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Prosthetic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: YAMADA Masahiro 東京歯科大学, 歯学部, 助教 (90549982)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 顎顔面補綴学 / 骨再生 / リン酸三カルシウム / 活性酸素 / 抗酸化 / 生体親和性 / 骨芽細胞 / 多机能化 / 多機能化

Research Abstract

Osteoblastic viability and functional expression on beta-tricalcium phosphate(β-TCP) were improved by application of anti-oxidant amino acid derivative, N-acetyl cysteine(NAC), on the material. It was indicated that NAC enhanced bone regeneration onβ-TCP material.

Research Products

• [Journal Article] N-acetyl Cysteine Improves Affinity of Beta-Tricalcium Phosphate Granules for Cultured Osteoblast-like Cells (2012)
  • Author(s): Yamada M, Minamikawa H, Ueno T, Sakurai K, Ogawa T
  • Journal Title: J Biomater Appl. Volume: (In press) Issue: 1 Pages: 27-36
  • DOI: 10.1177/0885328210383598
  • Peer Reviewed
• [Journal Article] The inhibition of infection by wound pathogens on scaffold in tissue-forming process using N-acetyl cysteine (2011)
  • Author(s): Yamada, M., Ishihara, K., Ogawa, T., Sakurai, K
  • Journal Title: Biomaterials Volume: 32 Issue: 33 Pages: 8474-8485
  • DOI: 10.1016/j.biomaterials.2011.07.074
  • Peer Reviewed
• [Journal Article] Improvement in the osteoblastic cellular response to a commercial collagen membrane and demineralized freeze-dried bone by an amino acid derivative : an in vitro study (2011)
  • Author(s): Yamada M, Kojima N, Att W, Minamikawa H, Sakurai K, Ogawa T
  • Journal Title: Clin Oral Implants Res. Volume: 22巻 Issue: 2 Pages: 165-72
  • DOI: 10.1111/j.1600-0501.2010.01975.x
  • Peer Reviewed
• [Journal Article] Effect of N-acetylcysteine on rat dental pulp cells cultured on mineral trioxide aggregate (2011)
  • Author(s): Minamikawa H, Yamada M, Deyama Y, Suzuki K, Kaga M, Yawaka Y, Ogawa T
  • Journal Title: J Endod. Volume: 37巻 Pages: 637-41
  • Peer Reviewed
• [Journal Article] Synergistic effects of UV photofunctionalization and micro-nano hybridtopography on the biological properties of titanium (2011)
  • Author(s): Tsukimura N., Yamada M., Iwasa F., Minamikawa H., Att W., Ueno T., Saruwatari L., Aita H., Chiou WA., Ogawa T.
  • Journal Title: Biomaterials Volume: 32 Issue: 19 Pages: 4358-68
  • DOI: 10.1016/j.biomaterials.2011.03.001
  • Peer Reviewed
• [Journal Article] Effect of N-acetylcysteine on rat dental pulp cells cultured on mineral trioxide aggregate (2011)
  • Author(s): Minamikawa H, Yamada M, Deyama Y, Suzuki K, Kaga M, Yawaka Y, Ogawa T
  • Journal Title: J Endod. Volume: 37 Issue: 5 Pages: 637-641
  • DOI: 10.1016/j.joen.2011.02.012
  • Peer Reviewed
• [Journal Article] Enhanced bone-integration capability of alkali- and heat-treated nanopolymorphic titanium in micro-to-nanoscale hierarchy (2011)
  • Author(s): Ueno T, Tsukimura N, Yamada M, Ogawa T
  • Journal Title: Biomaterials Volume: 32 Issue: 30 Pages: 7297-308
  • DOI: 10.1016/j.biomaterials.2011.06.033
  • Peer Reviewed
• [Journal Article] Bone integration capability of alkali- and heat-treated nanobimorphic Ti-15Mo-5Zr-3Al (2011)
  • Author(s): Tsukimura N., Ueno T., Iwasa F., Minamikawa H., Sugita Y., Ishizaki K., Ikeda T., Nakagawa K., Yamada M., Ogawa T
  • Journal Title: Acta Biomater Volume: 7 Issue: 12 Pages: 4267-77
  • DOI: 10.1016/j.actbio.2011.08.016
  • Peer Reviewed
• [Journal Article] Nanometer-thin TiO_2 enhances skeletal muscle cell phenotype and behavior (2011)
  • Author(s): Ishizaki K., Sugita Y., Iwasa F., Minamikawa H., Ueno T., Yamada M., Suzuki T., Ogawa T.
  • Journal Title: Int J Nanomedicine Volume: 6 Pages: 2191-203
  • DOI: 10.2147/ijn.s24839
  • Peer Reviewed
• [Journal Article] Alleviation of commercial collagen sponge-and membrane-induced apoptosis and dysfunction in cultured osteoblasts by an amino acid derivative (2010)
  • Author(s): Yamada M, Kubo K, Ueno T, Iwasa F, Att W, Hori N, Ogawa T
  • Journal Title: Int J Oral Maxillofac Implants. Volume: 25巻 Pages: 939-46
  • Peer Reviewed
• [Journal Article] Amino acid derivative-mediated detoxification and functionalization of dual cure dental restorative material for dental pulp cell mineralization (2010)
  • Author(s): Minamikawa H, Yamada M, Iwasa F, Ueno T, Deyama Y, Suzuki K, Yawaka Y, Ogawa T
  • Journal Title: Biomaterials Volume: 31巻 Pages: 7213-25
  • Peer Reviewed
• [Journal Article] コラーゲン創被覆用スポンジによる培養骨芽細胞のアポトーシス誘導および機能障害とN-アセチルシステインによるその改善 (2010)
  • Author(s): 山田将博、上野剛史、岩佐文則、堀紀雄、小川隆広
  • Journal Title: 日本口腔インプラント学会誌 Volume: 23 Pages: 3-11
  • NAID: 10026271179
  • Peer Reviewed
• [Journal Article] N-acetyl cysteine alleviates cytotoxicity of bone substitute (2010)
  • Author(s): Yamada, M., Ueno, T., Minamikawa, H., Sato, N., Hori, N., Ogawa T.
  • Journal Title: Journal of Dental Research Volume: 89 Pages: 411-416
  • Peer Reviewed
• [Journal Article] Improvement in osteoblastic cellular response to commercial collagen membrane and demineralized freeze-dried bone by amino acid derivative : an in vitro study (2010)
  • Author(s): Yamada, M., Kojima, N., Att, W., Minamikawa, H., Sakurai, K., Ogawa, T.
  • Journal Title: Clinical Oral Implants Research Volume: 22 Pages: 165-172
  • Peer Reviewed
• [Journal Article] Alleviation of commercial collagen sponge- and membrane-induced apoptosis and dysfunction in cultured osteoblasts by an amino acid derivative (2010)
  • Author(s): Yamada, M., Kubo, K., Ueno, T., Iwasa, F., Att, W., Hori, N., Ogawa, T.
  • Journal Title: The International Journal of Oral & Maxillofacial Implants Volume: 25 Pages: 939-946
  • Peer Reviewed
• [Presentation] 組織再生を促す抗酸化アミノ酸誘導体による生体材料の多機能化 (2011)
  • Author(s): 山田将博
  • Organizer: 第64回日本酸化ストレス学会学術集会シンポジウム
  • Place of Presentation: 北海道
  • Year and Date: 2011-07-03
• [Presentation] 組織再建術後合併症への酸化ストレスの関与と抗酸化アミノ酸誘導体による生体材料の多機能化 (2011)
  • Author(s): 山田将博
  • Organizer: 第31回日本歯科薬物療法学会シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2011-06-25
• [Presentation] Anti-infective functionalizaton of scaffold mediate by antioxidant amino acid derivative resisting wound infection pathogens (2011)
  • Author(s): Yamada M., Ishihara K., Sakurai K.
  • Organizer: 第291回東京歯科大学学術大会
  • Place of Presentation: 千葉
  • Year and Date: 2011-06-04
• [Presentation] 歯肉線維芽細胞による線維芽細胞増殖因子の傍分泌効果を介した骨芽細胞および骨膜細胞の機能発現亢進 (2011)
  • Author(s): 山田将博, 船登彰芳, 小川隆広, 櫻井薫
  • Organizer: 第41回日本口腔インプラント学会学術大会
  • Place of Presentation: 千葉
  • Year and Date: 2011-06-04
• [Presentation] Anti-infection capability of anti-oxidant amino acid derivative on bone biomaterial (2011)
  • Author(s): Yamada M., Sakurai K.
  • Organizer: the 120th Commemorative Scientific Meeting of Japan Prosthodontic Society
  • Place of Presentation: 広島
  • Year and Date: 2011-05-20
• [Presentation] Anti-infection capability of amino acid derivative for bone regeneration (2011)
  • Author(s): Yamada M., Ishihara K., Ogawa T., Sakurai K.
  • Organizer: IADR 89th General Session and Exhibition
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-16
• [Presentation] N-acetyl cysteine protects function of TMJ chondrocytes from oxidative stress (2011)
  • Author(s): Ueno T., Sugita Y., Yamada M., Minamikawa H., Iwasa F., Igarashi Y., Ogawa T.
  • Organizer: IADR 89th General Session and Exhibition
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-16
• [Presentation] Amino-acid Derivative-mediated Multifunctionalization of Biomaterial for BoneRegeneration (2010)
  • Author(s): Yamada M.
  • Organizer: BIT Life Sciences' 8th Annual Congress of International Drug Discovery Science and Technology
  • Place of Presentation: Beijing、China
  • Year and Date: 2010-10-24
• [Presentation] Amino-acid Derivative-mediated Multifunctionalization of Biomaterial for Bone Regeneration (2010)
  • Author(s): Yamada, M.
  • Organizer: BIT Life Sciences' 8th Annual Congress of International Drug Discovery Science and Technology
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-10-24
• [Presentation] アミノ酸誘導体の骨再生における細菌感染予防効果 (2010)
  • Author(s): 山田裕介、山田将博、山崎真, 内山宙, 櫻井薫
  • Organizer: 第40回日本口腔インプラント学会学術大会
  • Place of Presentation: 札幌(北海道)
  • Year and Date: 2010-09-17
• [Presentation] Amino Acid-derivative Mediated Multifunctionalization of PMMA and Bone Substitute (2010)
  • Author(s): Yamada M.
  • Organizer: The 88th General Session and Exhibition of the IADR
  • Place of Presentation: Barcelona、Spain
  • Year and Date: 2010-07-15
• [Presentation] Amino Acid-derivative Mediated Multifunctionalization of PMMA and Bone Substitute (2010)
  • Author(s): Yamada, M.
  • Organizer: the 88th General Session and Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] Detoxification of Collagenous Bone Graft Material by N-acetyl Cysteine (2010)
  • Author(s): Kojima, N., Yamada, M., Sakurai, K., Tanaka, K., Ogawa, T.
  • Organizer: the 88th General Session and Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] N-acetyl Cysteine-Mediated Detoxification and Functionalization of Resin-Modified Glass Ionomer Cement (2010)
  • Author(s): Minamikawa, H., Yamada, M., Ueno, T., Iwasa, F., Ogawa, T.
  • Organizer: the 88th General Session and Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Book] インプラント周囲炎を治療する-エビデンスに基づく診断・治療とリスクコントロール- (2011)
  • Author(s): 山田将博, 他
  • Total Pages: 221
  • Publisher: 医学情報社
• [Book] インプラント周囲炎を治療する-エビデンスに基づく診断・治療とリスクコントロール- (2010)
  • Author(s): 山田将博
  • Total Pages: 221
  • Publisher: 医学情報社
• [Remarks] 東京歯科大学HRCプロジェクト研究HRC第8プロジェクト・免疫機能・トランスレーショナル研究グループホームページ
  • URL: http://www.tdc.ac.jp/dept/osc/hrc8/hrc8g2ac.html
• [Remarks] 東京歯科大学有床義歯補綴学講座ホームページ
  • URL: http://www.tdc.ac.jp/dept/rpg/
• [Remarks]
  • URL: http://jglobal.jst.go.jp/public/20090422/200901020123698281