| Project/Area Number |
22791943
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 再生歯学 / 歯根膜細胞 / 筋細胞 / ニューロン / 再生医療 / mdxマウス / 脊髄損傷マウス / パッチクランプ / 活動電位 / 細胞移植 / 脊髄損傷 |
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| Research Abstract |
The muscle and nerve cells(neurons) were isolated from rat periodontal ligament cells. The muscle cells were confirmed by patch-clamp method, their action potential were similar to the skeletal muscle cells or the cardiac muscle. In immunostaining, there are positive cells for the skeletal muscle marker, toroponin T ; or the myocardial marker, toroponin I ; or the smooth muscle marker,α-smooth muscle action. In the evaluation of bone differentiation potential, the formation of calcification nodule were formed in the fibroblast from primary culture of periodontal ligament. But almost none of such calcification was observed in muscle cells and neurons.
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