Bone regeneration by layered osteoblast cell sheets - rhBMP

• Project/Area Number: 22791945
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: HAYASHI Tatsuhide 愛知学院大学, 歯学部, 講師 (70367621)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 再生歯学 / 骨再生 / 骨芽細胞シート / 積層化 / rhBMP-2 / rhBMP

Research Abstract

The aim of this study is to promote bone defect healing by layered osteoblast cell sheets implantation. First of all, temperature-responsive culture dishes were used to obtain cell sheets of rat osteoblast cells (v-1 cell). However, only partial cell sheets were obtained. Furthermore, it was quite difficult to make layered osteoblast cell sheets using these partial cell sheets. These results suggest that it depends on the type of cell cultured whether cell the sheet was successfully obtained or not.

Research Products

• [Presentation] Bone-like tissue induced by rhBMP-2, rhBMP-4 and rhBMP-7 in vitro has ossification potential after implantation. (2012)
  • Author(s): T. Hayashi, S. kobayashi, A. Mieki, H. Kataoka, T. Kawai.
  • Organizer: 3rd TERMIS World Congress2012
  • Place of Presentation: Vienna (Austria)
  • Year and Date: 2012-09-05
• [Presentation] Promotion of rat calvarial defect healing by induced bone-like tissue. (2012)
  • Author(s): T. Hayashi, S. Kobayashi, T. Asai, A. Mieki, H. Kataoka, Y. Uematsu, T. Kawai
  • Organizer: 90th IADR General Session & Exhibition
  • Place of Presentation: Iguacu Falls (Brazil)
  • Year and Date: 2012-06-20
• [Presentation] Promotion of rat calvarial defect healing by induced bone-like tissue (2012)
  • Author(s): Tatsuhide Hayashi
  • Organizer: 90th IADR General Session & Exhibition
  • Place of Presentation: Iguacu Falls, Brazil
• [Presentation] Bone-like tissue induced by rhBMP-2, rhBMP-4 and rhBMP-7 in vitro has ossification potential after implantation (2012)
  • Author(s): Tatsuhide Hayashi
  • Organizer: 3rd TERMIS World Congress 2012
  • Place of Presentation: Vienna, Austria
• [Presentation] 未成熟筋組織-rhBMP-ePTFEによる骨誘導の可能性 (2011)
  • Author(s): 林達秀, 小林周一郎, 朝倉正紀, 桐山喬至, 佐々木敬一, 後藤陽一, 河村純, 河合達志
  • Organizer: 第33回日本バイオマテリアル学会
  • Place of Presentation: 京都
  • Year and Date: 2011-11-21
• [Presentation] Bone-like tissue induced by rhBMPs in vitro has an ossification potential in vivo (2011)
  • Author(s): Hayashi T, Asai T, Asakura M, Sasaki K, Uematsu Y, Mieki A, Kataoka H, Kawai T
  • Organizer: International Dental Materials Congresss 2011
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-05-29
• [Presentation] rhBMPs induce immature muscular tissue to differentiate into bone-like tissue. (2010)
  • Author(s): T.Hayashi, T.Asai, A.Mieki, H.Kataoka, S.Hamajima, Y.Sato, M.Asakura, T.Kawai
  • Organizer: The 88^<th> Interanatinal Association for Dental Research General Session and Exivision.
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-16