| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
In 2010, epithelial colonies were formed after I reconstructed epithelial. mesenchymal interactions in vitro by double-layer co-culture method that inoculated ameloblasts on mesenchymal cells. Therefore I introduced recombinant Shh and Wnt gene of tool kit genes these related odontogenic morphogenesis to mesenchymal cells and carried out similar experiment, analyzed influences those signals of Shh and Wnt gave adamant oblast.
I continued analyses as for 2011, when using mesenchymal cells introduced recombinant Shh and Wnt gene as feeder cells, epithelial colony count of ameloblasts increased dominantly, and diameter of a colony at the maximum was enlarged significantly. In addition, ameloblasts formed the nodules after two weeks culture when ameloblasts were inoculated on mesenchymal cells treated mitomycin C. Nodules were bigger significantly, Notch1 gene expression was inhibited that regulated cell differentiation in Shh and Wnt signaling groups. Wnt signal completely inhibited Runx2/Cbfa1 gene expression of ameloblasts. More this year, I produced the mixture of collagen scaffold, mesenchymal cell and ameloblast, and transplanted the mixtures into back of nude mice subcutaneously. I do not reached tooth regeneration, because of oncogenic change of transplanted mixture.
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