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Investigation on the formation of immune privilege in tissue-engineered cartilage and its application on cartilage regenerative medicine

Research Project

Project/Area Number 22791956
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionThe University of Tokyo

Principal Investigator

FUJIHARA Fujihara  東京大学, 医学(系)研究科(研究院), 特任助教 (50466744)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords再生医療 / 再生軟骨 / 免疫特権
Research Abstract

Involvement of immune privilege in the transplantation of tissue-engineered cartilage was examined in mice. FasL and TGF-βwere expressed on chondrocytes of tissue-engineered cartilage constructs two weeks after transplantation. The constructs of FasL-hypomorphic mice(gld) showed more infiltration of F4/80-positive cells with less accumulation of proteoglycan than those of wild-type mice. When gld chondrocytes were cultured with macrophage-like cell, RAW264, they showed modest cytotoxicity and less induction of apoptosis on RAW264 than those of wild-type mice, suggesting that expression of FasL on chondrocytes could modulate the viability and localization of macrophages, promoting the maturation of tissue-engineered cartilage.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (9 results)

  • [Journal Article] BMP-2-embedded atelocollagen scaffold for tissue-engineered cartilage cultured in the medium containing insulin and Triiodothyronine-A new protocol for 3D in vitro culture of human chondrocytes2012

    • Author(s)
      Ko EC, et al.
    • Journal Title

      Tissue Eng Part C Methods

      Volume: (掲載確定)(in press) Issue: 5 Pages: 374-86

    • DOI

      10.1089/ten.tec.2011.0217

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the implant type tissue-engineered cartilage by scanning acoustic microscopy2012

    • Author(s)
      Tanaka Y, et al.
    • Journal Title

      J Biosci Bioeng

      Volume: (掲載確定)(in press) Issue: 2 Pages: 252-7

    • DOI

      10.1016/j.jbiosc.2011.10.011

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tissue responses against tissue-engineered cartilage consisting of chondrocytes encapsulated within nonabsorbable hydrogel2011

    • Author(s)
      Kanazawa S, et al.
    • Journal Title

      J Tissue Eng Regen Med

      Volume: 97(2) Issue: 1 Pages: 186-92

    • DOI

      10.1002/term.458

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Administration of the insulin into the scaffold atelocollagen for tissue-engineered cartilage2011

    • Author(s)
      Ko EC, Fujihara Y, Ogasawara T, Asawa Y, Nishizawa S, Nagata S, Takato T, Hoshi K
    • Journal Title

      J Biomed Mater Res A

      Volume: 97(2) Issue: 2 Pages: 186-92

    • DOI

      10.1002/jbm.a.33046

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Administration of the insulin into the scaffold atelocollagen for tissue engineered cartilage2011

    • Author(s)
      Ko E, et al.
    • Journal Title

      J Biomed Mater Res A

      Volume: (in press(掲載確定))

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Utility of NucleoCounter for the chondrocyte count in the collagenase digest of human native cartilage2010

    • Author(s)
      Yonenaga K, Nishizawa S, Akizawa M, Asawa Y, Fujihara Y, Takato T, Hoshi K
    • Journal Title

      Cytotechnology

      Volume: 62(6) Issue: 6 Pages: 539-45

    • DOI

      10.1007/s10616-010-9304-y

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The optimal conditions of chondrocyte isolation and its seeding in the preparation for cartilage tissue engineering2010

    • Author(s)
      Yonenaga K, Nishizawa S, Fujihara Y, Asawa Y, Sanshiro K, Nagata S, Takato T, Hoshi K
    • Journal Title

      Tissue Eng Part C Methods

      Volume: 16(6) Issue: 6 Pages: 1461-9

    • DOI

      10.1089/ten.tec.2009.0597

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The optimization of porous polymeric scaffolds for chondrocyte/atelocollagen based tissue-engineered cartilage2010

    • Author(s)
      Tanaka Y, Yamaoka H, Nishizawa S, Nagata S, Ogasawara T, Asawa Y, Fujihara Y, Takato T, Hoshi K
    • Journal Title

      Biomaterials

      Volume: 31(16) Issue: 16 Pages: 4506-16

    • DOI

      10.1016/j.biomaterials.2010.02.028

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effects of rapid- or intermediate-acting insulin on the proliferation and differentiation of cultured chondrocytes2010

    • Author(s)
      Iwata K, Asawa Y, Fujihara Y, Tanaka Y, Nishizawa S, Nakagawa T, Nagata S, Takato T, Hoshi K
    • Journal Title

      Curr Aging Sci

      Volume: 3 Issue: 1 Pages: 26

    • DOI

      10.2174/1874609811003010026

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effects of rapid- or intermediate-acting insulin on the proliferation and differentiation of cultured chondrocytes2010

    • Author(s)
      Iwata K, et al.
    • Journal Title

      Curr Aging Sci

      Volume: 3 Pages: 26-33

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The optimization of porous polymeric scaffolds for chondrocyte/atelocollagen based tissue-engineered cartilage2010

    • Author(s)
      Tanaka Y, et al.
    • Journal Title

      Biomaterials

      Volume: 31 Pages: 4506-4516

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Utility of NucleoCounter for the chondrocyte count in the collagenase digest of human native cartilage2010

    • Author(s)
      Yonenaga K, et al.
    • Journal Title

      Cytotechnology

      Volume: 62 Pages: 539-545

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The optimal conditions of chondrocyte isolation and its seeding in the preparation for cartilage tissue engineering2010

    • Author(s)
      Yonenaga K, et al.
    • Journal Title

      Tissue Eng Part C Methods

      Volume: 16 Pages: 1461-1469

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 耳介軟骨細胞におけるFas ligandの発現メカニズムの検討と再生軟骨移植への応用2011

    • Author(s)
      藤原夕子, ほか
    • Organizer
      第10回再生医療学会総会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-02
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 再生軟骨組織における免疫特権因子の発現と機能2011

    • Author(s)
      藤原夕子, ほか
    • Organizer
      第七回医工連携研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-02-02
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Chondrocytes in tissue-engineered cartilage using scaffolds could suppress tissue reactions through formation of immune privilege2011

    • Author(s)
      Fujihara Y et al
    • Organizer
      10th World Congress on Inflammation
    • Place of Presentation
      Paris France
    • Related Report
      2011 Final Research Report
  • [Presentation] 軟骨細胞に発現するMIFは、再生軟骨組織におけるマクロファージの局在制御を介して、軟骨成熟にに貢献する2011

    • Author(s)
      藤原夕子, ほか
    • Organizer
      第40回日本免疫学会学術集会
    • Place of Presentation
      千葉
    • Related Report
      2011 Annual Research Report
  • [Presentation] Chondrocytes in tissue-engineered cartilage using scaffolds could suppress tissue reactions through formation of immune privilege2011

    • Author(s)
      Fujihara Y, et al.
    • Organizer
      10th World Congress on Inflammation
    • Place of Presentation
      Paris, France
    • Related Report
      2011 Annual Research Report
  • [Presentation] Chondrocytes could suppress the viability of macrophages, modulating tissue reactions in tissue-engineered cartilage2010

    • Author(s)
      Fujihara Y et al
    • Organizer
      Regulatory Myeloid Cells
    • Place of Presentation
      Virginia USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Fas ligand on chondrocytes could worsen viability of macrophages, modulating tissue reactions in tissue-engineered cartilage2010

    • Author(s)
      Fujihara Y et al
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Japan(Kobe)
    • Related Report
      2011 Final Research Report
  • [Presentation] Chondrocytes could suppress the viability of macrophages, modulating tissue reactions in tissue-engineered cartilage2010

    • Author(s)
      Fujihara Y, et al.
    • Organizer
      Regulatory Myeloid Cells
    • Place of Presentation
      USA.ARLINGTON. VA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Fas ligand on chondrocytes could worsen viability of macrophages, modulating tissue reactions in tissue-engineered cartilage2010

    • Author(s)
      Fujihara Y, et al.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Japan (Kobe)
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2018-11-21  

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