| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
By assuming the expression of HBp17 in an oral cancer cell line, we treated them with 1α, 25(OH)_2D_3(VD_3). We investigated the expression of HBp17, FGF-2 and NF-κB molecules. Reporter assays were done for proof of down-regulation of these molecules.
The HBp17 expression was down-regulated with VD3. There are no changes in NF-κB expression(p65 or p50). However the expression of IκB-α was increased and its phosphorylation was decreased. Investigation on the HBp17 promoter activity by luciferase reporter assays showed the down-regulation of this molecule by VD3. We found that HBp17 expression in nuclear decreased by VD3 treatment with immunofluorescence cytochemistry. We concluded that VD3 down-regulated HBp17 expression by inhibiting DNA binding of NF-κB.
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