Molecular diagnosis of sentinel lymph node metastases for intraoperative detection in oral cancer

• Project/Area Number: 22791988
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Ehime University
• Principal Investigator: GODA Hiroyuki 愛媛大学, 医学部附属病院, 助教 (00464371)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: センチネルリンパ節 / 分子診断 / OSNA法 / OSNA / 口腔癌 / cytokeratin-19

Research Abstract

Sixty-one of 312 lymph nodes were pathologically metastasis-positive. The overall concordance rate between the OSNA assay using breast cancer criteria and histopathology was 94. 2%. The optimal cut-off for the copy number of CK19 mRNA in assessing lymph node metastasis of HNSCC was 300 copies/ll, which had the highest diagnostic accuracy(95. 2%). The OSNA assay can be completed within 30 min. And we identified ANXA8L2 and DSG3 markers by microarray analysis. These markers diagnosed metastatic lymph nodes in 100% sensitivity.

Research Products

• [Journal Article] One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma (2012)
  • Author(s): Hiroyuki Goda, Koh-ichi Nakashiro, Ryota Oka, Hiroshi Tanaka, Hiroyuki Wakisaka, Naohito Hato, Masamitsu Hyodo, Hiroyuki Hamakawa
  • Journal Title: Oral Oncology Volume: (in press)
  • Peer Reviewed
• [Presentation] 口腔扁平上皮癌センチネルリンパ節生検における分子診断の有用性 (2011)
  • Author(s): 岡亮太、中城公一、合田啓之、浜川裕之
  • Organizer: 第49回日本癌治療学会
  • Year and Date: 2011-10-27
• [Presentation] 口腔扁平上皮癌センチネルシンパ節生検における分子診断の有用性 (2011)
  • Author(s): 岡亮太、中城公一、合田啓之、浜川裕之
  • Organizer: 第49回日本癌治療学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-27
• [Presentation] Identification of molecular markers for sentinel lymph node biopsy of oral squamous cell carcinoma (2011)
  • Author(s): Ryota Oka, Koh-ichi Nakashiro, Hiroyuki Goda, Yukiko Nishikawa, Hiroshi Tanaka, Hiroyuki Hamakawa
  • Organizer: 3^<rd> World Congress of the International Academy of Oral Oncology
  • Place of Presentation: Singapore
  • Year and Date: 2011-07-14
• [Patent(Industrial Property Rights)] 頸部リンパ節転移分析方法及び頭頸部癌の腫瘍マーカー (2010)
  • Inventor(s): 浜川裕之, 中城公一, 合田啓之
  • Industrial Property Rights Holder: 愛媛大学
  • Filing Date: 2010-12-22
  • Overseas