| Project/Area Number |
22792000
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
AMEMIYA Takeshi 京都府立医科大学, 医学研究科, 助教 (90398389)
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| Research Collaborator |
NAKAMURA Takahiro 同志社大学, 生命医科学部, 准教授 (30411078)
KUMAMOTO Sonoko 京都府立医科大学, 医学部附属病院, 専攻医 (90613563)
YAMAMOTO Toshiro 京都府立医科大学, 医学研究科, 講師 (40347472)
KINOSHITA Shigeru 京都府立医科大学, 医学研究科, 教授 (30116024)
KANAMURA Narisato 京都府立医科大学, 医学研究科, 准教授 (70204542)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 口腔外科学一般 / 移植・再生医療 / 再生医学 / 細胞・組織 / 歯学 / 臨床 |
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| Research Abstract |
We developed the use of amniotic membrane tissue as a substrate for culturing periodontal ligament cells (PDL cells). Then, we cultured PDL cells on the tissue to develop a novel cultured PDL cell sheet. PDL cells, isolated and cultured from periodontal ligament tissue, were found to proliferate on the amniotic membrane tissue and form a sheet with tight cell-cell adhesion and a basement membrane. The cultured PDL cell sheet was placed on dentin slices for each amniotic membrane to be transplanted an experimental animal. The PDL cells adhered to the dentin slice, and the spindle-shaped periodontal ligament-like cells proliferated in several layers. Thus, the features of the periodontal ligament were retained even after transplantation.
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