Metabolisms of extracellular matrix in temporomandibular joint

• Project/Area Number: 22792002
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: ARIYOSHI Wataru 九州歯科大学, 歯学部, 准教授 (40405551)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 顎関節 / 細胞外マトリックス / アグリカナーゼ / ヒアルロン酸 / 変形性関節症 / アグリカン / ADAMTS4 / ADAMTS5 / 関節軟骨

Research Abstract

Hyaluronan(HA) oligosaccharides serve as competitive receptor antagonists to displace HA from the cell surface and induce cell signaling events. We determined changes in the expression and function of aggrecanases after disruption of chondrocyte CD44.HA interactions. Disruption of chondrocyte CD44.HA interactions with HA oligosaccharides induced the transcription of ADAMTS-4 and ADAMTS-5.The association of GPI. anchored MT4-MMP with ADAMTS-4 was also induced in articular chondrocytes by HA oligosaccharides. Inhibition of the NF-kappa B pathway blocked HA oligosaccharides-mediated stimulation of aggrecanases. These results suggest that disruptive changes in chondrocyte. matrix interactions by HA oligosaccharides induce matrix degradation and elevate aggrecanases via the activation of the NF-kappa B signaling pathway.

Research Products

• [Journal Article] Mechanisms involved in enhancement of the expression and function of aggrecanases by hyaluronan oligosaccharides (2012)
  • Author(s): Ariyoshi W, Tanakashi N, Hida D, Knudson CB, Knudson W
  • Journal Title: Arthritis & Rheumatism Volume: 64(1) Issue: 1 Pages: 187-97
  • DOI: 10.1002/art.33329
• [Journal Article] Mechanisms involved in enhancement of osteoblast differentioation by hyaluronic acid (2011)
  • Author(s): Kawano M, Ariyoshi W, Iwanaga K, Okinaga T, Habu M, Yoshioka I, Tominaga K, Nishihara T
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 405(4) Issue: 4 Pages: 575-80
  • DOI: 10.1016/j.bbrc.2011.01.071
  • Peer Reviewed
• [Journal Article] Mechanisms involved in regulation of osteoclastic differentiation by mechanical stress-loaded osteoblasts (2011)
  • Author(s): Kaneuji T, Ariyoshi W, Okinaga T, Toshinaga A, Takahashi T, Nishihara T
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 408(1) Pages: 103-109
  • Peer Reviewed
• [Journal Article] Role of heme oxygenase-1 in inflammatory response induced by mechanical stretch in synovial cells (2011)
  • Author(s): Takao M, Okinaga T, Ariyoshi W, Iwanaga K, Nakamichi I, Yoshioka I, Tominaga K, Nishihara T
  • Journal Title: Inflammation Research Volume: 60(9) Pages: 861-867
  • Peer Reviewed
• [Journal Article] Inhibition of adjuvant arthritis in rats by electroporation with interleukin-1 receptor antagonist (2011)
  • Author(s): Sukedai M, Ariyoshi W, Okinaga T, Iwanaga K, Habu M, Yoshioka I, Tominaga K, Nishihara T
  • Journal Title: Journal of Interferon & Cytokine Research Volume: 31(11) Issue: 11 Pages: 839-846
  • DOI: 10.1089/jir.2011.0024
  • Peer Reviewed
• [Journal Article] Novel histone deacetylate inhibitor exhibits antitumor activity via apoptosis induction in oral squamous cell carcinoma (2011)
  • Author(s): Takahashi O, Okinaga T, Ariyoshi W, Tominaga K, Nishihara T
  • Journal Title: Journal of Biophysical Chemistry Volume: 2(3) Issue: 03 Pages: 215-221
  • DOI: 10.4236/jbpc.2011.23026
  • Peer Reviewed
• [Journal Article] Dual effects of heparin on BMP-2-induced osteogenic activity in MC3T3-E1 cells (2011)
  • Author(s): Kanzaki S, Ariyoshi W, Takahashi T, Okinaga T, Kaneuji T, Mitsugi S, Nakashima K, Tsujisawa T, Nishihara T
  • Journal Title: Pharmacological Reports Volume: 63(5) Pages: 1222-1230
  • Peer Reviewed
• [Journal Article] Mechanisms involved in regulation of osteoclastic differentiation by mechanical stress-loaded osteoblasts (2011)
  • Author(s): Kaneuji T, Ariyoshi W, 他4名
  • Journal Title: Biochemical and Biophysical Research Communications Volume: VOL.48 Pages: 103-109
  • Peer Reviewed
• [Journal Article] Role of heme oxygenase-1 in inflammatory response induced by mechanical stretch in synovial cells (2011)
  • Author(s): Takao M, Ariyoshi W, 他6名
  • Journal Title: Inflammation Research Volume: VOL.60 Pages: 861-867
  • Peer Reviewed
• [Journal Article] Inhibition of adjuvant arthritis in rats by electroporation with interleukin-1 receptor antagonist (2011)
  • Author(s): Sukedai M, Ariyoshi W, 他6名
  • Journal Title: Journal of Interferon & Cytokine Research Volume: VOL.31 Pages: 839-846
  • Peer Reviewed
• [Journal Article] Dual effects of heparin on BMP-2-induced osteogenic activity in MC3T3-E1 cells (2011)
  • Author(s): Kanzaki S, Ariyoshi W, 他7名
  • Journal Title: Pharmacological Reports Volume: VOL.63 Pages: 1222-1230
  • Peer Reviewed
• [Journal Article] Mechanisms involved in enhancement of the expression and function of aggrecanases by hyaluronan oligosaccharides (2011)
  • Author(s): Ariyoshi W, 他4名
  • Journal Title: Arthritis & Rheumatism Volume: VOL.64 Pages: 187-97
  • Peer Reviewed
• [Journal Article] Mechanisms involved in enhancement of osteoblast differentiation by hyaluronic acid (2011)
  • Author(s): Kawano, et al
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 405(4) Pages: 575-580
  • Peer Reviewed
• [Journal Article] Hyaluronan oligosaccharide treatment of chondrocytes stimulates expression of both HAS-2 and MMP-3, but by different signaling pathways (2010)
  • Author(s): Schmitz I, Ariyoshi W, Takahashi N, Knudson CB, Knudson W
  • Journal Title: Osteoarthritis cartilage Volume: 18(3) Issue: 3 Pages: 447-54
  • DOI: 10.1016/j.joca.2009.10.007
  • Peer Reviewed
• [Journal Article] Induction of CD44 cleavage in articular chondrocytes (2010)
  • Author(s): Takahashi N, Knudson CB, Thankamony S, Ariyoshi W, Mellor L, Im HJ, Knudson W
  • Journal Title: Arthritis and Rheumatism Volume: 62(5) Issue: 5 Pages: 1338-48
  • DOI: 10.1002/art.27410
  • Peer Reviewed
• [Journal Article] Internalization of aggrecan G1 domain neoepitope ITEGE in chondrocytes requires CD44 (2010)
  • Author(s): Ariyoshi W, Knudson CB, Luo Na, Fosang AJ, Knudson W
  • Journal Title: The Journal of Biological Chemistry Volume: 285(46) Issue: 46 Pages: 36216-36224
  • DOI: 10.1074/jbc.m110.129270
  • Peer Reviewed
• [Journal Article] Hyaluronan oligosaccharide treatment of chondrocytes stimulates expression of both HAS-2 and MMP-3, but by different signaling pathways (2010)
  • Author(s): Schmitz I, et al
  • Journal Title: Journal of Cellular Physiology Volume: 216(3) Pages: 447-454
  • Peer Reviewed
• [Journal Article] Induction of CD44 cleavage in articular chondrocytes (2010)
  • Author(s): Takahashi N, et al
  • Journal Title: Arthritis and Rheumatism Volume: 62(5) Pages: 1338-1348
  • Peer Reviewed
• [Presentation] 牛関節軟骨細胞において表層部細胞は、昼間部/深部細胞と比較して強いHAオリゴ糖への反応性を示す (2012)
  • Author(s): 高橋伸典、有吉渉, 他2名
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: ウィルあいち(愛知県)
  • Year and Date: 2012-03-09
• [Presentation] 活性化マクロファージのプロテアーゼ産生に及ぼすHAの作用について (2011)
  • Author(s): 片岡良浩、有吉渉, 他4名
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-01
• [Presentation] 前軟骨細胞に対するアクチビンとヒアルロン酸の作用について (2011)
  • Author(s): 三次翔、有吉渉, 他4名
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-01
• [Presentation] Enamel matrix derivative中に含まれる増殖抑制因子の同定とその生物学的活性について (2011)
  • Author(s): 西藤法子、有吉渉, 他5名
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-01
• [Presentation] 高分子量ヒアルロン酸による破骨細胞支持能制御機構 (2011)
  • Author(s): 有吉渉、沖永敏則、西原達次
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2011-07-30
• [Presentation] ヒアルロン酸はRho kinaseを介して破骨細胞支持能を制御する (2011)
  • Author(s): 有吉渉、沖永敏則、西原達次
  • Organizer: 第71回九州歯科学会総会
  • Place of Presentation: 九州歯科大学講堂(福岡県)
  • Year and Date: 2011-05-28
• [Presentation] 前軟骨細胞の分化に及ぼすアクチビンの影響について (2011)
  • Author(s): 三次翔、有吉渉, 他5名
  • Organizer: 第71回九州歯科学会総会
  • Place of Presentation: 九州歯科大学講堂(福岡県)
  • Year and Date: 2011-05-28
• [Presentation] Effects of HDAC inhibitor in oral squamous cell carcinoma (2011)
  • Author(s): OSAMU TAKAHASHI, TOSHINORI OKINAGA, KENJIRO IWANAGA, WATARU ARIYOSHI, MANABU HABU, KAZUHIRO TOMINAGA, TATSUJI NISHIHARA
  • Organizer: 89^<th> General Session & Exhibition of the IADR
  • Place of Presentation: San Diego, United States
• [Presentation] Mechanical stress inhibits osteoclast differentiation through noncanonical Wnt pathway (2011)
  • Author(s): TAKESHI KANEUJI, WATARU ARIYOSHI, TOSHINORI OKINAGA, TETSU TAKAHASHI, TATSUJI NISHIHARA
  • Organizer: 89^<th> General Session & Exhibition of the IADR
  • Place of Presentation: San Diego, United States
• [Presentation] The role of SOCS in periodontal bacterium-infected macrophages (2011)
  • Author(s): TOSHINORI OKINAGA, WATARU ARIYOSHI, TATSUJI NISHIHARA
  • Organizer: 89^<th> General Session & Exhibition of the IADR
  • Place of Presentation: San Diego, United States
• [Presentation] Induction of HO-1 by mechanical stretch in synovial cells (2011)
  • Author(s): MASANOBU TAKAO, TOSHINORI OKINAGA, WATARU ARIYOSHI, KENJIRO IWANAGA, MANABU HABU, KAZUHIRO TOMINAGA, TATSUJI NISHIHARA
  • Organizer: 89^<th> General Session & Exhibition of the IADR
  • Place of Presentation: San Diego, United States
• [Presentation] Hyaluronan oligosaccharide up-regulate aggrecanase expression and function (2011)
  • Author(s): WATARU ARIOSHI, TOSHINORI OKINAGA, TATSUJI NISHIHARA
  • Organizer: 89^<th> General Session & Exhibition of the IADR
  • Place of Presentation: San Diego, United States
• [Presentation] ヒアルロン酸オリゴ糖によるアグリカナーゼ発現および、アグリカン分解活性の増強作用 (2011)
  • Author(s): 有吉渉, 他
  • Organizer: 第24回日本軟骨代謝学会
  • Place of Presentation: 九州大学百年講堂(福岡県)
• [Presentation] Turnover of aggrecan G1 domains is dependent on CD44-mediated endocytosis (2010)
  • Author(s): WATARU ARIYOSHI, NA LUO, DAISUKE HIDA, CHERYL B KNUDSON, WARREN KNUDSON
  • Organizer: Hyaluronan 2010
  • Place of Presentation: Kyoto, Japan
• [Presentation] Chondrocytes exhibit proteolytic fragmentation of CD44 (2010)
  • Author(s): WARREN KNUDON, NOBUNORI TAKAHASHI, WATARU ARIYOSHI, LILIANA MELLOR, CHERYL B KNUDSON
  • Organizer: Hyaluronan 2010
  • Place of Presentation: Kyoto, Japan
• [Presentation] The turnover of chondrocyte CD44 relies on multiple pathways (2010)
  • Author(s): DAISUKE HIDA, NOBUNORI TAKAHASHI, WATARU ARIYOSHI, CHERYL B KNUDSON, WARREN KNUDSON
  • Organizer: Hyaluronan 2010
  • Place of Presentation: Kyoto, Japan
• [Presentation] Hyaluronan oligosaccharide induce endogenous BMP-7 synthesis via transcriptional activation of NF-κB and p38 MAP kinase pathways in articular chondrocytes (2010)
  • Author(s): NOBUNORI TAKAHASHI, WATARU ARIYOSHI, CHERYL B KNUDSON, WARREN KNUDSON
  • Organizer: Hyaluronan 2010
  • Place of Presentation: Kyoto, Japan
• [Presentation] Effect of Hyaluronic acid and BMP-2 on osteoblasts differentiation (2010)
  • Author(s): MICHINAO KAWANO, TOSHINORI OKINAGA, KENJIRO IWANAGA, WATARU ARIYOSHI, MANABU HABU, KAZUHIRO TOMINAGA, TATSUJI NISHIHARA
  • Organizer: 88 the General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
• [Presentation] CD44 mediated internalization of aggrecan G1 domein by chondrocytes (2010)
  • Author(s): WATARU ARIYOSHI, TOSHINORI OKINAGA, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] SOCS regulates cell cycle arrest in periodontopathic bacterium-infected macrophages (2010)
  • Author(s): TOSHINORI OKINAGA, WATARU ARIYOSHI, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] Hyaluronic acid induces osteogenesis in periosteal cell (2010)
  • Author(s): SHO MITSUGI, WATARU ARIYOSHI, TOSHINORI OKINAGA, TETSU TAKAHASHI, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] The investigation of HO-1 in mechanical stretch induced inflammation (2010)
  • Author(s): MASANOBU TAKAO, TOSHINORI OKINAGA, WATARU ARIYOSHI, KENJIRO IWANAGA, MANABU HABU, KENJIRO OKINAGA, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] Hyaluronic acid enhances BMP-2 induced osteogenesis in MG63 cells (2010)
  • Author(s): MICHINAO KAWANO, WATARU ARIYOSHI, KENJIRO IWANAGA, TOSHINORI OKINAGA, MANABU HABU, KAZUHIRO TOMINAGA, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] Regulatory effect on osteoclast differentiation of soluble factors (2010)
  • Author(s): TAKESHI KANEUJI, WATARU ARIYOSHI, TOSHINORI OKINAGA, TETSU TAKAHASHI, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] HDAC inhibitor induces cell cycle arrest in myeloma cells (2010)
  • Author(s): SEIKO FUJII, TOSHINORI OKINAGA, WATARU ARIYOSHI, OSAMU TAKAHAHSI, KENJIRO IWANAGA, MANABU HABU, KAZUHIRO TOMINAGA, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] Effects of lipoproteins on the plaque formation of moraine macrophages (2010)
  • Author(s): MASATERU MORISHITA, KEISUKE NAKASHIMA, TOSHINORI OKINAGA, WATARU ARIYOSHI, MAKOTO YOKOTA, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] Purified fraction of EMD inhibits the proliferation of epithelial cells (2010)
  • Author(s): SACHIKO FUJIMOTO, NORIKO SAITO, WATARU ARIYOSHI, TOSHINORI OKINAGA, KAZUNORI YAMAGUCHI, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] Role of pili in S. sanguinis to cell adhesion (2010)
  • Author(s): RYOSHUKE SAEKI, MASATERU MORISHITA, TOSHINARI MAEDA, HIROAKI OGAWA, TOSHINORI OKINAGA, WATARU ARIYOSHI, TATSUJI NISHIHARA
  • Organizer: 58^<th> annual Meeting of JADR
  • Place of Presentation: Kitakyushu, Japan
• [Presentation] Turnover of aggrecan G1 domains is dependent on CD44-mediated endocytosis (2010)
  • Author(s): Ariyoshi W, et al
  • Organizer: Hyaluronan 2010
  • Place of Presentation: 京都ブライトンホテル(京都府)
• [Presentation] ヒアルロン酸およびアグリカンG1ドメインの取り込みにおけるCD44の役割 (2010)
  • Author(s): 有吉渉, 他
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル(東京都)
• [Presentation] CD44 Mediated Internalization of Aggrecan G1 Domain by Chondrocytes (2010)
  • Author(s): Ariyoshi W, et al
  • Organizer: 58th Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: 九州歯科大学記念講堂(福岡県)
• [Presentation] Hyaluronan oligosaccharides up-regulate aggrecanase expression and function (2010)
  • Author(s): Ariyoshi W, et al
  • Organizer: The 89th General Session & Exhibition of IADR
  • Place of Presentation: San Diego Convention Center (San Diego, USA)
• [Book] 微生物学滅菌と消毒 (2012)
  • Author(s): 井上博雅、有吉渉
  • Publisher: クインテッセンス出版株式
• [Book] 「微生物学」滅菌と消毒 (2012)
  • Author(s): 井上博雅、有吉渉
  • Total Pages: 7
  • Publisher: クインテッセンス出版株式会社
• [Book] 「口腔の病気と全身の健康」口腔内細菌による病気 (2011)
  • Author(s): 西原達次、沖永敏則、有吉渉
  • Total Pages: 7
  • Publisher: 学校法人福岡歯科学園福岡歯科大学
• [Book] 月刊糖尿病歯周病が関連する疾患4感染症 (2010)
  • Author(s): 有吉渉、沖永敏則、西原達次
  • Publisher: 医学出版
• [Book] 月刊糖尿病歯周病が関連する疾患4感染症 (2010)
  • Author(s): 西原達次、沖永敏則、有吉渉
  • Publisher: 医学出版