Development of next-generation therapy for temporomandibular joint arthrosis by gene transfer technique using nanofullerene and ultrasonic wave

Research Project

Project/Area Number	22792004
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Surgical dentistry
Research Institution	Kyushu Dental College
Principal Investigator	BASUGI Akihiko 九州歯科大学, 歯学部, 助教 (80351922)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords	ソノポレーション / フラーレン / ナノバブル / 遺伝子導入 / 顎関節炎 / マイクロバブル
Research Abstract	We develop the next-generation therapy for temporomandibular joint arthrosis by gene transfer techmique using the nanofullerene and ultrasonic wave. HIG-82 cells were seeded into silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The amounts of IL-1β, IL-1ra, PGE2 released into the culture medium were determined using ELISA. Nanofullerene pretreatment suppressed PGE2 synthesis induced by mechanical stretch. We used sonoporation to transfect DNA-nanofullerene into HIG-82 cells and examined the transfection efficiency. Transfection efficiency was significantly increased when the cells were ultrasonicated with nanofullerene.

Report

(3 results)