Development of basic technology using iPS cells for immunotherapy of oral cancer

• Project/Area Number: 22792016
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Showa University
• Principal Investigator: SATO Hana 昭和大学, 歯学部, 助教 (50551222)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: iPS細胞 / 口腔癌 / 免疫療法 / 樹状細胞 / 破骨細胞 / 遺伝子 / 細胞分化 / 再生 / がん / 免疫 / 骨吸収

Research Abstract

iPS(induced pluripotent stem) cells are multipotent cells induced from somatic cells by transfection of 4 genes. In this study, we tried to establish a basic technology using iPS cells useful for development of new immunotherapy of oral cancer. Mouse iPS cells obtained from Kyoto University were subjected to induce myeloid dendritic cell and osteoclast differentiations, which are known to relate to bone metastasis of oral cancer. iPS cells were cocultured with some osteoblastic cell lines in the presence of various kinds of differentiation factors. The coculture conditions such as, cell numbers in culture wells, concentrations of differentiation factors, and incubating periods were examined. We found that osteoblastic UAMS-32 cell line supports the differentiation of dendritic cell-like cells with phagocytic potential. Furthermore, elongation of the coculture periods induced differentiation of osteoclast-like multinucleated giant cells with bone resorbing capacity. Differentiation efficiency of dendritic cells and osteoclasts depended on cell types of osteoblastic cell lines. These results suggest a possibility to use iPS cells-derived dendritic cells in immunotherapy of oral cancer. In addition, development of new drugs that inhibit osteoclast differentiation from iPS cells would be useful to improve immunotherapy and suppression of bone metastasis of oral cancer.

Research Products

• [Journal Article] Enhancement of bone morphogenetic protein-2-induced ectopic bone formation by transforming growth factor (2010)
  • Author(s): Tachi K, Takami M, Sato H, Mochizuki A, Zhao B, Miyamoto Y, Tsukasaki H, Inoue T, Shintani S, Koike T, Honda Y, Suzuki O, Baba K, Kamijo R
  • Journal Title: Tissue Engineering
• [Remarks]
  • URL: http://www.omfs-showa.jp/