| Project/Area Number |
22792040
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
HARUYAMA Naoto 東京医科歯科大学, 歯と骨のGCOE拠点, GCOE拠点形成特任教員 (70359529)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞外マトリクス / 象牙質シアロリン酸化タンパク / 骨代謝 / 間葉系幹細胞 / 歯学 / 細胞外マトリックス / 破骨細胞 / 骨芽細胞 / 歯科矯正学 / 象牙シアロリン酸化タンパク |
|---|
| Research Abstract |
In this study, we examined the effects of dentin sialophosphoprotein (DSPP), one of the major extracellular matrix proteins in dentin, on bone turnover in vivo and in vitro. According to micro CT analysis, DSPP knock out (KO) mouse femora showed significantly decreased bone mineral density as compared to those in DSPP wild type (WT) mice. However, calvarial organ culture analysis revealed that the decreased osteoclastogenesis in DSPP KO as compared to WT calvaria. Using bone marrow stromal cells (BMSCs) isolated from both WT and KO mice, we found that under the osteogenic media,ALP activity and in vitro mineralization were higher in BMSCs derived from WT mice as compared to those from KO mice. On the other hand, the KO derived BMSCs showed increased differentiation into adipocytes as compared to those from WT mice. Thus, the DSPP may be a positive regulator for the bone mineral density, promoting the bone formation but not suppressing the bone resorption.
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