Direct imaging of flow-induced oscillatory calcium rise in living bone
| Project/Area Number |
22792051
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 歯科矯正学 / 骨細胞 / 骨芽細胞 / 細胞内カルシウム / ギャップ結合 / オシレーション |
|---|
| Research Abstract |
Bone cells form a complex three-dimensional network consisting of osteoblasts and osteocytes embedded in a mineralized extracellular matrix. Ca^<2+> acts as a ubiquitous secondary messenger in various physiological cellular processes and transduces numerous signals to the cell interior and between cells. However, the intracellular Ca^<2+> dynamics of bone cells have not been evaluated in living bone. In the present study, we developed a novel ex-vivo live Ca^<2+> imaging system that allows the dynamic intracellular Ca^<2+> concentration([Ca^<2+>] i) responses of intact chick calvaria explants to be observed without damaging the bone network. Our live imaging analysis revealed for the first time that both osteoblasts and osteocytes display repetitive and autonomic[Ca^<2+>] i oscillations ex vivo.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] Roles of Heparan Sulfate Sulfation in Dentinogenesis2012
Author(s)
Satoru Hayano, Hiroshi Kurosaka, Takeshi Yanagita, Ina Kalus, Fabian Milz, Yoshihito Ishihara, Md. Nurul Islam, Noriaki Kawanabe, Masahiro Saito, Hiroshi Kamioka, Taiji Adachi, Thomas Dierks, Takashi Yamashiro
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Journal Title
Journal of Biological Chemistry
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] Stage-specific embryonic antigen-4 identifies human dental pulp stem cells2012
Author(s)
Noriaki Kawanabe, Satoko Murata, Hiroaki Fukushima, Yoshihito Ishihara, Takeshi Yanagita, Emmy Yanagita, Mitsuaki Ono, Hiroshi Kurosaka, Hiroshi Kamioka, Tomoo Itoh, Takuo Kuboki, Takashi Yamashiro
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Journal Title
Experimental Cell Research
Volume: 318(5)
Pages: 453-63
Related Report
Peer Reviewed
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