Analysis of the mechanism of FGF-2 enhancement induced by extracellular calcium in cementoblasts

• Project/Area Number: 22792079
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Tohoku University
• Principal Investigator: KANAYA Sousuke 東北大学, 病院, 医員 (80375097)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: セメント芽細胞 / 細胞外カルシウム / PMA / FGF-2 / プロテインキナーゼC / カルシウム

Research Abstract

We found that extracellular calcium increases fibroblast growth factor-2 expression levels via a cAMP/Protein kinase A dependent pathway in cementoblasts. We also found that Phorbol 12-myristate 13-acetate (PMA), the potent stimulator of Protein kinase C increases FGF-2 expression in cementoblasts. These indicate the presence of different pathways for FGF-2 enhancement in cementoblasts.

Research Products

• [Journal Article] Elevated extracellular calcium increases fibroblast growth factor-2 gene and protein expression levels via a cAMP/PKAdependent pathway in cementoblasts (2010)
  • Author(s): Kanaya S, Nemoto E, Ebe Y, Somerman MJ, Shimauchi H
  • Journal Title: Bone Volume: 47(3) Pages: 564-572
  • NAID: 130004571423
  • Peer Reviewed
• [Journal Article] H. Shimauchi Elevated extracellular calcium increases expression of bone morphogenetic protein-2 gene via a calcium channel and ERK pathway in human dental pulp cells (2010)
  • Author(s): Tada H. Nemoto E, Kanaya S, Hamaji N, Sato H
  • Journal Title: Biochem. Biophys. Res. Commun Volume: vol.394 Pages: 1093-1097
  • Peer Reviewed
• [Journal Article] Elevated extracellular calcium increases fibroblast growth factor-2 gene and protein expression levels via a cAMP/PKA dependent pathway in cementoblasts. (2010)
  • Author(s): Sousuke Kanaya, Eiji.Nemoto, Yukari Ebe, Martha J.Somerman, Hidetoshi Shimauchi
  • Journal Title: Bone Volume: 47 Pages: 564-572
  • NAID: 130004571423
  • Peer Reviewed
• [Journal Article] Elevated extracellular calcium increases expression of bone morphogenetis protein-2 gene via a calcium channel and ERK pathway in human dental pulp cells. (2010)
  • Author(s): Hiroyuki Tada, Eiji Nemoto, Sousuke Kanaya, Nozomu Hamaji, Hisae Sato, Hidetoshi Shimauchi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 394 Pages: 1093-1097
  • Peer Reviewed
• [Presentation] セメント芽細胞におけるプロテインキナーゼC依存性Fibroblast growth factor 2発現増強作用 (2010)
  • Author(s): 金谷聡介、根本英二、後藤和宏、島内英俊
  • Organizer: 第132回春季日本歯科保存学会学術大会
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-04
• [Presentation] セメント芽細胞において細胞外カルシウム刺激はcAMP/PKA依存性にFibroblast growth factor 2の発現を誘導する (2010)
  • Author(s): 金谷聡介、根本英二、江部由佳梨、島内英俊
  • Organizer: 第53回春季日本歯周病学会学術大会
  • Place of Presentation: 岩手
  • Year and Date: 2010-05-14